FastQCFastQC Report
Fri 10 Feb 2017
ERR1632774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404222
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76001.8801549643512723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56941.408631890396861No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28790.7122323871535938No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA24490.6058551983810876No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG23190.573694652938237No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20850.5158056711411056No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16150.3995329299246454No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.3708358278371786No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT13440.33249056211685657No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC13220.3270480082726819No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT13120.3245741201616933No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11320.28004413416390006No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA11290.2793019677306035No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11110.27484896913082413No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11050.273364636264231No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC10770.26643774955346317No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10730.2654481943090678No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10650.26346908382027695No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10480.2592634740315965No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10360.2562948082984103No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10230.2530787537541252No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA10180.2518418096986309No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9580.23699848103269985No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT9250.22883465026643776No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8850.2189390978224837No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8840.21869170901138482No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8720.2157230432781986No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT8700.21522826565600092No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8540.21127004467841928No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8430.20854876775633194No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8420.20830137894523307No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC8160.20186926985666293No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8000.1979110488790813No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7840.19395282790149967No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7810.19321066146820312No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7650.18925244049062148No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA7540.18653116356853414No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7380.1825729425909525No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT7300.18059383210216168No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7000.17317216776919614No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6910.17094566846930648No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6750.16698744749172487No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA6730.16649266986952715No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6640.16426617056963747No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6450.1595657831587593No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA6370.15758667266996848No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6370.15758667266996848No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6270.15511278455897995No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6170.15263889644799147No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6110.15115456358139834No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6020.14892806428150868No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5940.14694895379271786No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5930.14670156498161901No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5860.14496984330392707No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5830.14422767687063048No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5780.14299073281513625No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5780.14299073281513625No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.13680601253766494No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5480.13556906848217068No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5470.13532167967107184No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5460.13507429085997297No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC5360.13260040274898446No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5240.12963173701579825No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5120.12666307128261203No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5050.12493134960492008No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4980.1231996279272281No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4890.12097312862733844No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4890.12097312862733844No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4880.12072573981623959No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4760.11775707408305339No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC4760.11775707408305339No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4690.11602535240536141No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4690.11602535240536141No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA4660.11528318597206486No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4660.11528318597206486No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4660.11528318597206486No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4630.1145410195387683No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4580.11330407548327405No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4570.11305668667217518No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4560.11280929786107634No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4530.11206713142777977No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4510.11157235380558207No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4450.11008802093898898No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT4430.10959324331679128No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.1078615216390993No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4350.10761413282800046No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4320.1068719663947039No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4300.1063771887725062No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.10563502233920964No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4270.10563502233920964No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG4250.10514024471701194No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4190.10365591185041882No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT4140.10241896779492458No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC4060.10043985730613376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGG250.005492181529.5999987
CTAAGTC250.005492181529.59999834
TAAGTCT250.005492181529.59999835
CTAGTGG405.930751E-527.759
CGCTCTC2750.027.5818229
GAATCAA754.6020432E-1027.1333331
CCGCTCT2850.026.61403728
GGTATCA12150.026.4938281
CGAGCCC358.8594074E-426.42857215
GCAGCTA358.8594074E-426.42857230
GCTCTCT2900.026.15517230
GGCAGCT3050.026.08196818
CCGGCAG3050.026.08196816
GCCGCTC3000.025.927
ATTAGAG509.072064E-625.8999983
CTCTCTT2900.024.8793131
ATCTCGT451.3211397E-424.66666637
CAGCTTC3300.024.66666620
GCCGGCA3250.024.47692315
TATGAAA5750.024.1304362
Produced by FastQC (version 0.11.5)