FastQCFastQC Report
Fri 10 Feb 2017
ERR1632773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355595
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56661.5933857337701598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42831.2044601302043054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22340.6282428043138965No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG21730.6110884573742601No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA20010.5627188233805311No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18500.5202547842348739No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT16700.46963539982283214No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15520.43645158115271593No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC13750.38667585314754144No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG12320.3464615644201971No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA12040.33858743795610174No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC12000.33746256274694525No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11260.3166523713775503No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10920.3070909320997202No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC10860.3054036192859855No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10850.3051224004836963No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.29612339881044447No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10220.2874056159394817No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC10190.2865619595326144No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA9930.2792502706730972No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC9810.2758756450456278No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9640.2710949254067127No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9630.2708137066044236No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9440.26547054936093023No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT9080.25534667247852194No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA9080.25534667247852194No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC9000.25309692206020895No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8700.2446603579915353No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8640.24297304517780058No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8620.24241060757322236No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT8290.23313038709768136No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8250.23200551188852486No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8200.23059941787707924No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8040.22609991704045335No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7970.22413138542442945No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7680.21597604015804495No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7510.2111953205191299No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7180.2019151000435889No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7100.19966534962527596No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7090.19938413082298684No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6660.18729172232455463No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6620.18616684711539813No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA6460.1816673462787722No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6430.18082368987190484No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC6410.1802612522673266No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT6410.1802612522673266No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG6320.1777302830467245No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA6260.1760429702329898No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT6230.17519931382612242No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA6210.17463687622154417No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6120.17210590700094208No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.17182468819865296No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5990.1684500625711835No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC5970.16788762496660528No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5960.16760640616431613No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5920.16648153095515966No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC5920.16648153095515966No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA5920.16648153095515966No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5780.16254446772311196No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5700.160294717304799No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC5670.15945106089793162No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG5530.15551399766588395No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5330.14988962162010153No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5240.14735865239949944No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5210.14651499599263207No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA5190.14595255838805382No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5150.14482768317889735No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG5130.1442652455743191No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC5120.14398402677202998No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5080.1428591515628735No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5070.1425779327605844No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4770.13414136869191073No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4750.1335789310873325No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG4710.13245405587817602No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4700.1321728370758869No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT4660.1310479618667304No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4610.12964186785528478No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4560.12823577384383922No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4540.12767333623926097No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4430.12457992941408062No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC4400.12373627300721327No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA4400.12373627300721327No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4350.12233017899576765No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4310.12120530378661118No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4300.12092408498432204No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC4280.12036164737974381No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4230.1189555533682982No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4210.11839311576371996No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4150.11670580294998523No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT4140.11642458414769612No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC4130.11614336534540698No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4120.11586214654311787No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC3980.11192508331107018No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG3970.11164386450878107No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3970.11164386450878107No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT3960.11136264570649193No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3910.10995655169504634No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.1096753328927572No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA3720.10461339445155303No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3670.10320730044010742No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA3670.10320730044010742No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG3670.10320730044010742No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3630.10208242523095094No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC3610.1015199876263727No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3570.10039511241721623No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3560.10011389361492709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAATA308.295834E-637.02
GATGCTA251.2319113E-437.014
CTCTAAT251.2319113E-437.01
GAATCAA352.3837041E-531.7142871
TGGGCAG352.3837041E-531.71428734
TTAGGGT303.5945233E-430.8333342
TAGAGGT303.5945233E-430.83333419
GTGATGC303.5945233E-430.83333412
GTCACTG303.5945233E-430.83333429
GTGTCAC303.5945233E-430.83333427
ACATGTG303.5945233E-430.83333423
GGTATCA8900.029.9325871
CGTATGC250.005491302329.636
ATGGACC250.005491302329.632
TGGCCGG250.005491302329.636
AGTAATA250.005491302329.62
AGGAGCC405.9287726E-527.7526
AGAGGTG405.9287726E-527.7520
TGTCACT358.8573E-426.42857228
GGTACCG358.8573E-426.4285726
Produced by FastQC (version 0.11.5)