FastQCFastQC Report
Fri 10 Feb 2017
ERR1632772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326679
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48541.4858622684653742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37801.1570991707455944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19460.5956917953097689No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG19220.5883451339082096No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18330.5611012645440937No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16480.5044707495737406No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT13580.41569859097156536No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13260.40590304243615294No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10920.33427309377094944No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10690.3272325432611218No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9880.302437561030859No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9770.29907034122181103No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9640.29509089962929974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.29386645606237316No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9310.28498924020215566No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA9000.27549980255847484No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8850.27090813918250023No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8630.26417369956440423No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8610.26356147778094097No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8460.25896981440496636No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA8440.2583575926215031No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8270.2531537074620652No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8180.25039870943648046No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7900.2418276044679946No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7750.23723594109201998No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7710.23601149752509343No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7620.2332564994995087No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7510.2298892796904607No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT7410.2268281707731443No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7310.2237670618558279No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7060.21611428956253695No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6980.21366540242868381No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6940.21244095886175726No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6900.21121651529483068No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6750.20662485191885613No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6590.2017270776511499No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6210.19009486376534765No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6200.189788752873616No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6160.18856430930668944No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG6130.18764597663149454No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5940.18182986968859338No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5930.18152375879686175No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5930.18152375879686175No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5580.1708098775862544No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5530.16927932312759622No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.16560599242681653No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5400.1652998815350849No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5380.1646876597516216No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT5230.16009599637564703No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC5050.15458600032447753No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA5020.15366766764928264No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4940.1512187805154295No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4930.15091266962369787No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4870.14907600427330805No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4850.14846378248984476No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4850.14846378248984476No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4830.1478515607063815No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4780.1463210062477233No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4770.14601489535599166No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4720.14448434089733347No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4700.14387211911387018No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4680.1432598973304069No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4600.1408110101965538No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4560.13958656662962726No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4470.1368315686040425No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4450.13621934682057923No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA4440.13591323592884758No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG4440.13591323592884758No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4270.13070935076940973No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG4250.13009712898594644No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4200.12856657452728826No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4120.12611768739343515No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4120.12611768739343515No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4010.12275046758438712No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3940.12060769134226565No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3940.12060769134226565No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC3920.11999546955880236No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3830.11724047153321762No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3820.11693436064148599No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3820.11693436064148599No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3750.11479158439936452No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3740.11448547350763288No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3730.11417936261590124No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT3640.11142436459031649No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3620.11081214280685321No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC3570.10928158834819501No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3550.10866936656473174No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3540.1083632556730001No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT3540.1083632556730001No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3500.10713881210607354No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3420.10468992497222043No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3390.10377159229702551No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC3390.10377159229702551No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC3380.10346548140529388No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT3370.10315937051356223No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA3370.10315937051356223No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC3320.10162881605490406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG200.001840007837.034
TCGAAAA200.001840007837.013
AGATCTT352.3831151E-531.7142878
GATCTTG352.3831151E-531.7142879
TCTTAGG250.00549065829.59999829
GATCTAG250.00549065829.59999821
AGAGGTG250.00549065829.59999820
GGTATCA7300.028.6369861
TAGATCT405.9273167E-527.7500027
GTGACAT405.9273167E-527.75000216
GTATCAA23250.026.4967731
GGGCTTC1100.025.22727237
GCAGCTT2000.024.97499819
ATTATAG451.320381E-424.6666683
GGCAGCT2050.024.36585418
GCCGCTC2050.024.36585427
GAATCAA551.8972589E-523.5454541
GCCGGCA2200.023.54545415
TTATAGT551.8972589E-523.54545412
TATAGTT551.8972589E-523.54545413
Produced by FastQC (version 0.11.5)