FastQCFastQC Report
Fri 10 Feb 2017
ERR1632771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407836
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27810.6818917407977717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20130.4935807530477937No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC13770.33763571631734324No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12650.3101736972704715No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG11330.277807746250944No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11290.27682695985641287No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11100.27216822448239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10500.25745642856442297No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC10360.254023676183564No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT10250.25132651359860336No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10250.25132651359860336No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10110.24789376121774437No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT10040.24617738502731493No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9920.2432350258437215No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9690.23759550407516744No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9470.23220117890524625No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9180.22509047754489547No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8460.20743632244333504No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8220.20155160407614825No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC8140.199590031287086No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA8090.19836404829392207No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC8010.19640247550485979No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT7950.1949312959130631No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG7910.19395050951853196No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG7900.19370531291989918No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT7580.18585902176365007No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC7510.1841426455732206No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC7470.1831618591786895No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG7310.17923871360056493No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7300.17899351700193214No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG7070.1733539952333781No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6970.17090202924705028No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA6510.15962298570994224No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6430.15766141292087996No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT6380.15643542992771603No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC6320.15496425033591935No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6210.1522670877509587No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG6000.14711795917967024No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5970.1463823693837719No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT5850.1434400102001785No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5830.14294961700291295No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA5810.14245922380564738No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5640.13829088162889003No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC5490.13461293264939828No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT5480.13436773605076552No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5450.13363214625486716No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC5430.1331417530576016No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG5380.13191577006443767No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA5310.13019939387400817No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5310.13019939387400817No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC5250.12872821428221148No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT5100.1250502653027197No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA5020.12308869251365746No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG4910.12039152992869684No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4780.11720397414647064No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG4730.11597799115330672No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT4710.11548759795604116No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT4670.11450681156151002No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4670.11450681156151002No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT4660.11426161496287725No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT4640.11377122176561166No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4620.11328082856834609No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4560.11180964897654941No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4550.11156445237791661No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG4530.11107405918065105No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG4450.10911248639158878No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC4420.10837689659569043No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA4400.10788650339842486No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG4380.1073961102011593No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT4370.1071509136025265No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC4350.10666052040526094No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4310.10567973401072979No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA4280.10494414421483146No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG4270.10469894761619868No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4170.10224698162987084No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC4110.10077580203807412No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4090.10028540884080855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTT250.005492236529.628
CGGGCAC250.005492236529.617
GCGGCTT250.005492236529.627
CCGGTCG250.005492236529.69
AAGTGGT250.005492236529.627
GGTATCA5200.028.8173081
GCTCCGT405.9308783E-527.7519
GGCTCCG358.859544E-426.4285718
GTACGTA358.859544E-426.4285713
TACGTAG358.859544E-426.4285714
TTTAGCA451.321168E-424.66666624
ATATAAT451.321168E-424.6666661
GCTACAT851.9663275E-923.94117723
GTATCAA15700.023.6847131
CAAAGCT551.898759E-523.54545424
CGCCGGC2000.023.12500214
TAGAGCT400.001929062823.12535
CGCATCT400.001929062823.12515
TTGACGC400.001929062823.12532
GACGCCG400.001929062823.12534
Produced by FastQC (version 0.11.5)