FastQCFastQC Report
Fri 10 Feb 2017
ERR1632770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453713
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25950.571947464586644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.42714226835025665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10390.22899938948189716No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8670.19108996215669377No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8330.1835962381505489No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG7860.17323726673028986No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7830.1725760557885712No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7710.16993121202169653No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7400.16309869895727033No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7020.15472336036216727No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6940.15296013118425084No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6820.15031528741737618No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6660.1467888290615433No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6640.1463480217670642No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5980.1318013810492536No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5930.13069936281305583No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5890.1298177482240976No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5750.12673209716274383No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT5630.12408725339586921No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5530.12188321692347365No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5460.12034039139279676No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5450.12011998774555722No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5430.11967918045107812No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5430.11967918045107812No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.11923837315659898No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5350.11791595127316166No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5190.11438949291732879No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA5180.11416908927008924No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG5120.1128466673866519No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5110.11262626373941237No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4930.10865899808910039No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4870.10733657620566305No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT4770.10513253973326751No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4750.1046917324387884No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG4660.10270809961363242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG200.001840762337.06
GGTATCA4350.033.1724131
GCTCTAG401.5972419E-632.3751
ACGCCAG303.596075E-430.83333433
CGAACAA250.005492880529.65
TTCAGTC250.005492880529.62
GACCGGG250.005492880529.67
TAACGAA250.005492880529.62
GTATAAA250.005492880529.61
ACGACCG250.005492880529.65
GTGCCTG250.005492880529.629
CGCCAGT250.005492880529.634
TACCGCC358.861089E-426.428578
GTATCAA16200.024.3240741
ATATAGC1300.024.192316
GCCGGCA1150.024.13043615
CCGGCAG1103.6379788E-1223.54545416
GTCGCCG952.8012437E-1023.36842224
CCAAGCA952.8012437E-1023.36842236
CAGTCTG400.001929395823.12537
Produced by FastQC (version 0.11.5)