FastQCFastQC Report
Fri 10 Feb 2017
ERR1632769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418536
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12790.30558900548578855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.21766347458761015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4910.11731368388860218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTATA551.8497303E-830.2727282
ATTAGCC358.8599336E-426.4285723
GGTATCA2700.025.3518521
GTATCAA7800.023.9551261
TAGAACC400.001929146623.1250024
AACCGTG806.9383714E-720.81258
CTAACCG806.9383714E-720.81256
TAACCGT806.9383714E-720.81257
AGACTAT450.003821804220.5555576
CTATACA450.003821804220.5555574
CTTTGCG450.003821804220.5555579
TCTACAC555.1355874E-420.1818183
TCAACGA555.1355874E-420.18181820
CTGGTCG500.007027590618.59
TAGTTCG500.007027590618.57
ACGATTA801.6126707E-518.523
CTAGGCC500.007027590618.53
GTCGCCC852.7164218E-517.41176437
CGCTGAA852.7164218E-517.41176427
AGGTCGC852.7164218E-517.41176435
Produced by FastQC (version 0.11.5)