FastQCFastQC Report
Fri 10 Feb 2017
ERR1632768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215579
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49062.2757318662764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38571.7891353053868884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19550.906860130161101No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11570.5366942049086414No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11490.5329832683146318No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG10300.4777830864787387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.42582997416260393No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8870.4114500948608167No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7730.3585692483961796No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7700.357177647173426No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7210.33444816053511706No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6970.3233153507530882No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6320.29316399092676004No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6070.28156731407047997No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5800.2690429030656975No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5630.26115716280342705No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.2606932957291759No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5470.2537352896154078No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5390.2500243530213982No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5360.24863275179864458No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5340.24770501765014216No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5150.23889154323936934No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5100.2365722078681133No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4880.22636713223458685No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4840.22451166393758207No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4790.222192328566326No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4610.21384272122980438No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA4530.2101317846357948No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4470.20734858219028757No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4290.19899897485376591No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4230.1962157724082587No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4150.19250483581424907No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC4020.18647456384898342No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3850.178588823586713No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3800.176269488215457No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3740.17348628576994976No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3720.17255855162144734No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3710.17209468454719618No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3640.16884761502743773No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3570.16560054550767933No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3570.16560054550767933No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3500.1623534759879209No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3490.1618896089136697No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3480.1614257418394185No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3470.1609618747651673No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG3390.15725093817115768No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3340.15493160279990165No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3340.15493160279990165No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3330.15446773572565045No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3310.15354000157714806No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.14982906498313842No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3230.14982906498313842No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3170.14704586253763122No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC3020.14008785642386318No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3010.13962398934961198No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2980.13823238812685837No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2940.13637691982985356No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2930.13591305275560234No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2920.13544918568135117No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2910.13498531860709995No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA2910.13498531860709995No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC2880.13359371738434633No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2880.13359371738434633No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2870.13312985031009514No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2840.13173824908734152No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2840.13173824908734152No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2820.13081051493883913No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2790.12941891371608552No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA2780.12895504664183433No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG2730.1266357112705783No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2730.1266357112705783No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2640.12246090760231747No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2620.12153317345381509No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2600.12060543930531267No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT2550.11828610393405666No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2520.11689450271130306No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2490.11550290148854944No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2460.11411130026579583No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG2420.11225583196879103No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2420.11225583196879103No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG2400.11132809782028863No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG2390.11086423074603742No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG2390.11086423074603742No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2380.11040036367178621No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2380.11040036367178621No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2340.10854489537478139No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2330.1080810283005302No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT2310.1071532941520278No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2280.10576169292927419No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2250.10437009170652058No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC2250.10437009170652058No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2240.10390622463226937No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG2210.10251462340951578No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2200.10205075633526457No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2190.10158688926101336No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.10158688926101336No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2190.10158688926101336No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC2160.10019528803825975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCAA308.280667E-637.01
CAATATT200.00183862137.04
CTAGTGC352.3793815E-531.7142859
TAGTGCA352.3793815E-531.71428510
ACTAGTG601.2587407E-930.8333328
CGTATGC250.00548656429.636
AAAAAGT250.00548656429.637
GTATGCC250.00548656429.637
GCAATAT250.00548656429.63
ATTGTTA453.9884253E-628.77777914
TATTGTT453.9884253E-628.77777913
CGTTATT453.9884253E-628.77777910
TGTTACG453.9884253E-628.77777916
TTGTTAC453.9884253E-628.77777915
GTTATTG453.9884253E-628.77777911
GGTATCA8850.028.6384181
GACCGTT652.9613147E-928.4615387
AGGACCG754.5656634E-1027.1333335
TTAGGAC706.528353E-926.428573
TAGGACC706.528353E-926.428574
Produced by FastQC (version 0.11.5)