FastQCFastQC Report
Fri 10 Feb 2017
ERR1632767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266275
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7530.28279034832410105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.19791568866773074No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3040.11416768378556003No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC2990.1122899258285607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2760.10365223922636371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTT251.2310795E-436.99999617
GAGTAAG250.005488855829.5999981
TCGATTT250.005488855829.59999813
CGCTCTC250.005488855829.59999829
GTAAGAC250.005488855829.5999983
GGTATCA1450.026.7931021
GCCGCTC358.851433E-426.4285727
AATACTA358.851433E-426.428575
AGCCGGT601.3309673E-624.66666831
TTAGCCG601.3309673E-624.66666829
AACGGCC952.7830538E-1023.36842237
TTAACGG952.7830538E-1023.36842235
GTATCAA4450.023.2808971
CCGCTCT400.00192731323.12500228
TTCGCCG400.00192731323.12500224
CGGTGCT652.6700109E-622.7692334
GAGTTAG652.6700109E-622.7692326
TAACGGC1005.293259E-1022.19999936
GTTAGCC705.077667E-621.14285728
TAGCCGG705.077667E-621.14285730
Produced by FastQC (version 0.11.5)