##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632767.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	266275
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68236597502582	33.0	33.0	33.0	27.0	33.0
2	31.224489719275187	33.0	33.0	33.0	27.0	33.0
3	31.63814101962257	33.0	33.0	33.0	27.0	33.0
4	31.774394892498357	33.0	33.0	33.0	27.0	33.0
5	31.88080743592151	33.0	33.0	33.0	33.0	33.0
6	34.737162707726974	37.0	37.0	37.0	27.0	37.0
7	34.852104027790816	37.0	37.0	37.0	27.0	37.0
8	35.06609708008638	37.0	37.0	37.0	33.0	37.0
9	35.101872124683126	37.0	37.0	37.0	33.0	37.0
10	35.171108816073605	37.0	37.0	37.0	33.0	37.0
11	35.201581072199794	37.0	37.0	37.0	33.0	37.0
12	35.12677119519294	37.0	37.0	37.0	33.0	37.0
13	35.18276218195474	37.0	37.0	37.0	33.0	37.0
14	35.15405126279222	37.0	37.0	37.0	33.0	37.0
15	35.174270960473194	37.0	37.0	37.0	33.0	37.0
16	35.18556004131067	37.0	37.0	37.0	33.0	37.0
17	35.15303727349545	37.0	37.0	37.0	33.0	37.0
18	35.11522298375739	37.0	37.0	37.0	33.0	37.0
19	35.15870810252559	37.0	37.0	37.0	33.0	37.0
20	35.098709980283544	37.0	37.0	37.0	33.0	37.0
21	35.15512909585954	37.0	37.0	37.0	33.0	37.0
22	35.16267017181485	37.0	37.0	37.0	33.0	37.0
23	35.13776734578913	37.0	37.0	37.0	33.0	37.0
24	35.12446155290583	37.0	37.0	37.0	33.0	37.0
25	35.151459956811564	37.0	37.0	37.0	33.0	37.0
26	35.105503708571966	37.0	37.0	37.0	33.0	37.0
27	35.04523894470003	37.0	37.0	37.0	27.0	37.0
28	35.01597596469815	37.0	37.0	37.0	27.0	37.0
29	35.05976903577129	37.0	37.0	37.0	33.0	37.0
30	35.01395549713642	37.0	37.0	37.0	27.0	37.0
31	35.00218571026195	37.0	37.0	37.0	27.0	37.0
32	35.03379213219416	37.0	37.0	37.0	27.0	37.0
33	35.011078771946295	37.0	37.0	37.0	27.0	37.0
34	34.965986292366914	37.0	37.0	37.0	27.0	37.0
35	34.95954933809032	37.0	37.0	37.0	27.0	37.0
36	34.93480048821707	37.0	37.0	37.0	27.0	37.0
37	34.96263261665571	37.0	37.0	37.0	27.0	37.0
38	34.80039808468688	37.0	37.0	37.0	27.0	37.0
39	34.89122148155103	37.0	37.0	37.0	27.0	37.0
40	34.93179983100178	37.0	37.0	37.0	27.0	37.0
41	34.95769035771289	37.0	37.0	37.0	27.0	37.0
42	34.8271223359309	37.0	37.0	37.0	27.0	37.0
43	34.647939160642196	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	20.0
17	5.0
18	15.0
19	10.0
20	17.0
21	49.0
22	184.0
23	412.0
24	782.0
25	1479.0
26	2377.0
27	3598.0
28	5002.0
29	6733.0
30	8625.0
31	10939.0
32	13866.0
33	17625.0
34	24971.0
35	45110.0
36	124452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.1397990798986	20.070603699183177	13.605107501643039	25.184489719275188
2	16.14496291428035	22.51168904328232	37.88226457609614	23.46108346634119
3	18.020843113322695	26.471129471411135	30.050136137451883	25.457891277814287
4	12.979438550370856	17.932964040935122	38.54999530560511	30.53760210308891
5	14.205614496291426	36.81644915970331	35.06412543423153	13.91381090977373
6	30.192094639001034	39.40174631490001	17.212656088630176	13.193502957468784
7	27.1482489907051	31.7070697587081	23.296591869308045	17.848089381278754
8	24.49009482677683	36.24786405032391	20.42737771101305	18.834663411886208
9	26.090320157731668	15.522673927330768	19.938409539010422	38.44859637592714
10	15.055112196037932	28.122429818796356	33.555159139986856	23.267298845178857
11	34.73927330767064	23.174913153694487	23.989860107032204	18.095953431602666
12	23.216599380339876	25.296779645103744	30.101586705473665	21.385034269082716
13	28.249741808280916	22.025349732419492	25.695615435170403	24.02929302412919
14	22.05276499859168	20.649328701530372	27.464463430663788	29.83344286921416
15	24.271148248990706	28.209182236409724	24.159609426344943	23.360060088254624
16	23.365693362125622	28.17801145432354	24.698901511595157	23.757393671955683
17	22.785466153412827	27.40249741808281	25.984790160548304	23.82724626795606
18	23.39723969580321	27.03483241010234	27.37658435827622	22.191343535818234
19	25.179607548586986	26.031734109473287	26.734391136982445	22.05426720495728
20	24.37329828185147	26.2807248145714	26.933433480424373	22.412543423152755
21	23.7142052389447	26.742653271993237	26.83879447939161	22.704347009670453
22	23.36306450098582	26.507182424185523	26.791850530466622	23.33790254436203
23	23.290958595437047	26.447845272744342	27.305980659093045	22.955215472725566
24	23.473476668857383	26.96235095296216	26.065533752699277	23.498638625481174
25	23.722091822364096	26.678433949863862	26.927049103370575	22.672425124401467
26	23.65937470660032	26.803117078208622	26.765186367477234	22.77232184771383
27	23.526804994836166	26.865458642380997	26.2927424654962	23.31499389728664
28	22.83015679278941	26.699464838982255	27.1426157168341	23.327762651394234
29	24.333865364754484	26.483898225518733	26.233029762463616	22.949206647263168
30	23.729602854192095	26.356210684442775	26.977373016618156	22.936813444746974
31	23.672519012299315	26.325791005539383	26.74002441085344	23.26166557130786
32	22.615716834100084	26.490282602572528	27.064125434231528	23.82987512909586
33	22.766313022251435	26.529339968078112	27.556473570556754	23.1478734391137
34	23.37620880668482	26.071167026570276	27.266923293587457	23.28570087315745
35	23.36907332644822	26.87184301943479	26.737771101305043	23.021312552811942
36	23.995493380903202	26.50342690827152	26.48765374143273	23.013425969392546
37	23.380339874190216	26.350201858980377	26.587174913153692	23.68228335367571
38	23.45620129565299	26.59769035771289	26.622852314336683	23.323256032297436
39	23.137733546145903	26.337808656464183	27.133226927049105	23.391230870340813
40	23.241761336963666	26.866960848746597	27.127969204769503	22.763308609520234
41	23.34165806027603	26.0028166369355	27.066754295371325	23.588771007417144
42	22.91202703971458	26.976621913435356	26.982630738897758	23.128720307952307
43	23.66275467092292	25.638907144869027	26.94470002816637	23.75363815604169
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	8.0
2	14.0
3	47.5
4	81.0
5	81.0
6	92.0
7	103.0
8	97.0
9	91.0
10	132.0
11	173.0
12	173.0
13	280.5
14	388.0
15	689.5
16	991.0
17	1135.5
18	1280.0
19	1280.0
20	1227.0
21	1174.0
22	1387.5
23	1601.0
24	2176.0
25	2751.0
26	2751.0
27	3690.5
28	4630.0
29	5689.5
30	6749.0
31	8297.5
32	9846.0
33	9846.0
34	11745.0
35	13644.0
36	15163.5
37	16683.0
38	17953.5
39	19224.0
40	19224.0
41	20146.5
42	21069.0
43	21538.5
44	22008.0
45	21803.5
46	21599.0
47	21599.0
48	21028.5
49	20458.0
50	19971.5
51	19485.0
52	18752.0
53	18019.0
54	18019.0
55	17266.5
56	16514.0
57	14542.0
58	12570.0
59	11643.0
60	10716.0
61	10716.0
62	9368.0
63	8020.0
64	6743.5
65	5467.0
66	4657.0
67	3847.0
68	3847.0
69	3261.5
70	2676.0
71	2182.0
72	1688.0
73	1387.5
74	1087.0
75	1087.0
76	883.5
77	680.0
78	534.0
79	388.0
80	321.5
81	255.0
82	255.0
83	189.0
84	123.0
85	115.5
86	108.0
87	76.0
88	44.0
89	44.0
90	35.0
91	26.0
92	13.5
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	266275.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.64723327308083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.45505210064024	64.80854218583251
2	5.473332358933244	7.842982406031837
3	1.4765168217449216	3.1736503547755888
4	0.6930123415963597	1.986096675979134
5	0.3903442294074014	1.3983542030576532
6	0.2571357743848072	1.105384008612154
7	0.18500845276133362	0.9278740640738116
8	0.1483324419593507	0.8502087256821858
9	0.11641144366228962	0.7506502073755352
>10	0.7034130566123791	9.881513319674571
>50	0.06780251917307964	3.273597074155257
>100	0.032587257276984014	3.5191262691799015
>500	0.0010512018476446455	0.48202050556985276
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	753	0.28279034832410105	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	527	0.19791568866773074	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	304	0.11416768378556003	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	299	0.1122899258285607	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	276	0.10365223922636371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.7555159139986854E-4	0.0	0.0
2	0.0	0.0	3.7555159139986854E-4	0.0	0.0
3	0.0	0.0	3.7555159139986854E-4	3.7555159139986854E-4	0.0
4	0.0	0.0	3.7555159139986854E-4	3.7555159139986854E-4	0.0
5	0.0	0.0	7.511031827997371E-4	3.7555159139986854E-4	0.0
6	0.0	0.0	7.511031827997371E-4	3.7555159139986854E-4	0.0
7	0.0	0.0	0.0022533095483992112	3.7555159139986854E-4	0.0
8	0.0	0.0	0.00262886113979908	7.511031827997371E-4	0.0
9	0.0	0.0	0.0030044127311989483	0.0011266547741996056	0.0
10	3.7555159139986854E-4	0.0	0.0045066190967984225	0.0015022063655994742	0.0
11	3.7555159139986854E-4	0.0	0.006008825462397897	0.0015022063655994742	0.0
12	3.7555159139986854E-4	0.0	0.007511031827997371	0.0015022063655994742	0.0
13	3.7555159139986854E-4	0.0	0.008637686602196977	0.0015022063655994742	0.0
14	3.7555159139986854E-4	0.0	0.008637686602196977	0.0015022063655994742	0.0
15	3.7555159139986854E-4	0.0	0.009388789784996713	0.0015022063655994742	0.0
16	3.7555159139986854E-4	0.0	0.010139892967796452	0.0015022063655994742	0.0
17	3.7555159139986854E-4	0.0	0.010139892967796452	0.0015022063655994742	0.0
18	3.7555159139986854E-4	0.0	0.010139892967796452	0.0015022063655994742	0.0
19	3.7555159139986854E-4	0.0	0.010139892967796452	0.0015022063655994742	0.0
20	3.7555159139986854E-4	0.0	0.01051544455919632	0.0015022063655994742	0.0
21	3.7555159139986854E-4	0.0	0.01051544455919632	0.0015022063655994742	0.0
22	3.7555159139986854E-4	0.0	0.01051544455919632	0.0022533095483992112	0.0
23	3.7555159139986854E-4	0.0	0.01051544455919632	0.00262886113979908	0.0
24	3.7555159139986854E-4	0.0	0.010890996150596188	0.003379964322598817	0.0
25	3.7555159139986854E-4	0.0	0.010890996150596188	0.003379964322598817	0.0
26	3.7555159139986854E-4	0.0	0.010890996150596188	0.0037555159139986854	0.0
27	3.7555159139986854E-4	0.0	0.010890996150596188	0.00525772227959816	0.0
28	3.7555159139986854E-4	0.0	0.010890996150596188	0.012017650924795793	0.0
29	3.7555159139986854E-4	0.0	0.010890996150596188	0.024035301849591587	0.0
30	3.7555159139986854E-4	0.0	0.010890996150596188	0.045441742559384095	0.0
31	3.7555159139986854E-4	0.0	0.010890996150596188	0.09989672331236503	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGTT	25	1.2310795E-4	36.999996	17
GAGTAAG	25	0.0054888558	29.599998	1
TCGATTT	25	0.0054888558	29.599998	13
CGCTCTC	25	0.0054888558	29.599998	29
GTAAGAC	25	0.0054888558	29.599998	3
GGTATCA	145	0.0	26.793102	1
GCCGCTC	35	8.851433E-4	26.42857	27
AATACTA	35	8.851433E-4	26.42857	5
AGCCGGT	60	1.3309673E-6	24.666668	31
TTAGCCG	60	1.3309673E-6	24.666668	29
AACGGCC	95	2.7830538E-10	23.368422	37
TTAACGG	95	2.7830538E-10	23.368422	35
GTATCAA	445	0.0	23.280897	1
CCGCTCT	40	0.001927313	23.125002	28
TTCGCCG	40	0.001927313	23.125002	24
CGGTGCT	65	2.6700109E-6	22.76923	34
GAGTTAG	65	2.6700109E-6	22.76923	26
TAACGGC	100	5.293259E-10	22.199999	36
GTTAGCC	70	5.077667E-6	21.142857	28
TAGCCGG	70	5.077667E-6	21.142857	30
>>END_MODULE