FastQCFastQC Report
Fri 10 Feb 2017
ERR1632766.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632766.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309462
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23810.7693997970671682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16920.5467553366810788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9940.32120260322753685No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG5720.1848369105092063No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4730.15284590676722828No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.15090705805559324No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG4070.13151857093924296No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4010.1295797222276079No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3450.11148380091901429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4000.027.28751
GCCGAGT358.8546926E-426.42857212
CGCTCCC358.8546926E-426.42857225
CGGTTGG509.063086E-625.90000233
TGGGGCG601.3321369E-624.66666637
GTATCAA10600.023.5613211
TTCGGTT551.8968398E-523.54545431
ATTTCGG551.8968398E-523.54545429
TAGCCGG1054.0017767E-1122.90476430
GTGTAAA502.6959236E-422.21
TAGGGCT502.6959236E-422.24
TTGCCGA603.7149708E-521.58333210
CGCTCTC957.0940587E-921.42105329
GTATTAG1400.021.1428591
AGCCGGT1151.3278623E-1020.91304431
GCCGGCA1151.3278623E-1020.91304415
CGGTGCT1151.3278623E-1020.91304434
TTAACGG806.926348E-720.812535
TTAGCCG1202.3646862E-1020.04166629
GCACGGA1202.3646862E-1020.04166621
Produced by FastQC (version 0.11.5)