FastQCFastQC Report
Fri 10 Feb 2017
ERR1632765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357234
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7960.22282313553581126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.14500299523561588No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3720.10413342514990175No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTCG303.5945565E-430.83333421
TTCGCCG303.5945565E-430.83333424
GGTATCA2100.030.8333321
GCCGCTC400.001928596523.12527
TCCAGCG400.001928596523.1252
CCGCTCT502.6970534E-422.19999928
CCTAGTC450.003820725520.5555552
CGCCGCT450.003820725520.55555526
TAAGAGT450.003820725520.5555554
TAACTCC555.133562E-420.1818184
GTATCAA5450.020.0275251
GTTTAGG759.236486E-619.7333341
CGTTGAA609.219835E-418.516
GCCTGTT701.2162147E-418.511
TACTTAA500.007025625618.4999987
CGCCTGT500.007025625618.49999810
TATAGTG1002.8611612E-718.4999985
GGGGTTA1156.368282E-817.6956526
TTTAGGA953.5923294E-617.5263162
ACAGTGG852.7146185E-517.4117648
Produced by FastQC (version 0.11.5)