##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632765.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357234
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.738261195742847	33.0	33.0	33.0	27.0	33.0
2	31.242622482742405	33.0	33.0	33.0	27.0	33.0
3	31.685164346058887	33.0	33.0	33.0	27.0	33.0
4	31.820148698052257	33.0	33.0	33.0	27.0	33.0
5	31.92550541101911	33.0	33.0	33.0	33.0	33.0
6	34.83043607271424	37.0	37.0	37.0	27.0	37.0
7	34.99505366230538	37.0	37.0	37.0	33.0	37.0
8	35.153120923540314	37.0	37.0	37.0	33.0	37.0
9	35.25835446793978	37.0	37.0	37.0	33.0	37.0
10	35.280186096508174	37.0	37.0	37.0	33.0	37.0
11	35.305091340689856	37.0	37.0	37.0	33.0	37.0
12	35.25384761808787	37.0	37.0	37.0	33.0	37.0
13	35.27903279083178	37.0	37.0	37.0	33.0	37.0
14	35.26290890564728	37.0	37.0	37.0	33.0	37.0
15	35.26959080042773	37.0	37.0	37.0	33.0	37.0
16	35.28024488150624	37.0	37.0	37.0	33.0	37.0
17	35.2450942519469	37.0	37.0	37.0	33.0	37.0
18	35.252305211709974	37.0	37.0	37.0	33.0	37.0
19	35.248016706136596	37.0	37.0	37.0	33.0	37.0
20	35.26059669572325	37.0	37.0	37.0	33.0	37.0
21	35.266444403388256	37.0	37.0	37.0	33.0	37.0
22	35.27506060453372	37.0	37.0	37.0	33.0	37.0
23	35.25118549746105	37.0	37.0	37.0	33.0	37.0
24	35.23977560926451	37.0	37.0	37.0	33.0	37.0
25	35.245928439062354	37.0	37.0	37.0	33.0	37.0
26	35.21327477227811	37.0	37.0	37.0	33.0	37.0
27	35.16300240178706	37.0	37.0	37.0	33.0	37.0
28	35.14258441245794	37.0	37.0	37.0	33.0	37.0
29	35.14804301942144	37.0	37.0	37.0	33.0	37.0
30	35.13564498340023	37.0	37.0	37.0	33.0	37.0
31	35.12177732242731	37.0	37.0	37.0	33.0	37.0
32	35.151304187171434	37.0	37.0	37.0	33.0	37.0
33	35.14211133318777	37.0	37.0	37.0	33.0	37.0
34	35.07308654831287	37.0	37.0	37.0	33.0	37.0
35	35.081652362317136	37.0	37.0	37.0	33.0	37.0
36	35.060179042308405	37.0	37.0	37.0	27.0	37.0
37	35.09581114899478	37.0	37.0	37.0	33.0	37.0
38	34.955760090025024	37.0	37.0	37.0	27.0	37.0
39	35.03278803249411	37.0	37.0	37.0	27.0	37.0
40	35.047053192025395	37.0	37.0	37.0	27.0	37.0
41	35.06401966218221	37.0	37.0	37.0	27.0	37.0
42	34.99254270310217	37.0	37.0	37.0	27.0	37.0
43	34.80637901207612	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	9.0
16	14.0
17	18.0
18	15.0
19	15.0
20	32.0
21	73.0
22	183.0
23	488.0
24	910.0
25	1680.0
26	2922.0
27	4378.0
28	6051.0
29	8449.0
30	10679.0
31	13928.0
32	17553.0
33	22961.0
34	32921.0
35	60303.0
36	173650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.11842657753741	20.323653403651388	13.392622202813842	25.165297815997356
2	16.360704748148272	22.433195048623592	37.73185083166776	23.474249371560376
3	17.864201055890536	26.568299769898722	29.898050017635498	25.66944915657524
4	12.88315221955357	17.82613077142713	38.861082651707285	30.429634357312015
5	13.972354255194075	37.04182692576855	35.390528337168355	13.595290481869027
6	30.110515796368766	39.42905770447382	17.3505321441968	13.109894354960613
7	27.043058611442362	31.72486381475447	23.589020082075056	17.64305749172811
8	24.712933259432194	35.97921810353998	20.35416561693456	18.953683020093273
9	25.81137293762632	15.559549203043382	20.097471125368806	38.531606733961496
10	14.959942222744754	28.10370793373531	33.83888431672237	23.09746552679756
11	34.43541208283646	23.452694872268598	23.997435854369964	18.114457190524977
12	23.16128923898621	25.50401137629677	30.035774870253114	21.29892451446391
13	28.154095074936876	22.041295061500303	25.59554801614628	24.20906184741654
14	22.090002631328485	20.678322891997965	27.23201039094823	29.999664085725325
15	23.862790215936894	28.787013554140984	23.798126718061553	23.552069511860573
16	23.441777658341593	28.062838363649593	24.898245967629062	23.59713801037975
17	22.70360603973866	27.46127188341535	26.13888935543649	23.696232721409498
18	23.37151558922163	27.258323675797936	26.99463097017641	22.37552976480402
19	24.892647396384444	26.11565528477133	26.675792337795396	22.31590498104884
20	24.26420777417603	26.49719791509207	26.779085977258603	22.459508333473295
21	23.494124299478774	26.92436890105645	26.716381979318875	22.865124820145898
22	23.061634670832003	26.831152689833555	26.700146122709484	23.40706651662496
23	23.004529244136897	26.54982448479148	27.473588740153517	22.97205753091811
24	23.245267807655488	27.16370782176389	25.9560960042997	23.634928366280924
25	23.54283186930695	26.61224855416898	27.076929967472303	22.76798960905177
26	23.351640661303236	26.904773901700285	26.95908004277309	22.784505394223395
27	23.10362395516664	26.936405829232378	26.4549287021952	23.50504151340578
28	23.208597166003237	26.45716813069305	27.23229031951046	23.101944383793256
29	24.247412060442176	26.566340269963106	26.352754776980913	22.833492892613805
30	23.631009366409693	26.568579698460955	26.76620926339598	23.034201671733374
31	23.648085008705777	26.41769820341849	26.96327897120655	22.970937816669185
32	22.76603010911615	26.625405196593828	26.968037756764474	23.640526937525543
33	22.85000867778543	26.611688697044517	27.479747168522593	23.058555456647465
34	23.46277230050891	26.13720978406311	27.002189041356644	23.397828874071337
35	23.20047923769854	26.89973518758013	26.95768039996193	22.9421051747594
36	24.02094985359736	26.540306913675632	26.213350352989917	23.225392879737093
37	23.27689973518758	26.186477211015745	26.873701831292657	23.662921222504014
38	23.60021722456429	26.61644748260244	26.517352771572693	23.265982521260575
39	22.968698388171337	26.572498698332186	27.01870482652827	23.440098086968206
40	23.135255882698736	26.819955547344314	27.126757251549407	22.918031318407543
41	23.10334402660441	25.957215718548625	27.133475537042944	23.805964717804017
42	22.79598246527486	26.87202225991927	26.936405829232378	23.39558944557349
43	23.436738944221435	25.85448193620988	27.188621463802438	23.520157655766248
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	6.0
2	7.0
3	39.5
4	72.0
5	72.0
6	97.5
7	123.0
8	120.0
9	117.0
10	150.5
11	184.0
12	184.0
13	290.0
14	396.0
15	702.0
16	1008.0
17	1207.5
18	1407.0
19	1407.0
20	1409.5
21	1412.0
22	1675.5
23	1939.0
24	2725.5
25	3512.0
26	3512.0
27	4785.0
28	6058.0
29	7683.0
30	9308.0
31	11478.5
32	13649.0
33	13649.0
34	15785.0
35	17921.0
36	20103.5
37	22286.0
38	24352.0
39	26418.0
40	26418.0
41	27930.0
42	29442.0
43	30070.0
44	30698.0
45	30230.5
46	29763.0
47	29763.0
48	28743.0
49	27723.0
50	27173.0
51	26623.0
52	25323.0
53	24023.0
54	24023.0
55	22759.0
56	21495.0
57	19000.5
58	16506.0
59	15195.0
60	13884.0
61	13884.0
62	12375.0
63	10866.0
64	9042.0
65	7218.0
66	6069.5
67	4921.0
68	4921.0
69	4057.5
70	3194.0
71	2631.0
72	2068.0
73	1648.0
74	1228.0
75	1228.0
76	967.5
77	707.0
78	556.5
79	406.0
80	334.0
81	262.0
82	262.0
83	206.0
84	150.0
85	133.0
86	116.0
87	98.5
88	81.0
89	81.0
90	57.0
91	33.0
92	18.0
93	3.0
94	2.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	357234.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.14104055570031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.21045907812214	58.34331551323019
2	6.410919248139038	8.480497399809678
3	1.8420759153919168	3.6551045347984665
4	0.9010460915605607	2.3838450433784923
5	0.5369034298503194	1.7755675764112286
6	0.3512432706066001	1.3938957243664776
7	0.25938988886202957	1.2009422011273467
8	0.19938796528475047	1.05501819985738
9	0.15330806683433973	0.9125959559405471
>10	1.0184386784730635	13.03286832524592
>50	0.08271789479494358	3.738079195505295
>100	0.033257710278308615	3.6577055575725916
>500	8.527618020079132E-4	0.37056477275639055
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	796	0.22282313553581126	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	518	0.14500299523561588	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	372	0.10413342514990175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.7992856223091865E-4	0.0	0.0
2	0.0	0.0	2.7992856223091865E-4	0.0	0.0
3	0.0	0.0	2.7992856223091865E-4	0.0	0.0
4	0.0	0.0	5.598571244618373E-4	0.0	0.0
5	0.0	0.0	5.598571244618373E-4	0.0	0.0
6	0.0	0.0	8.39785686692756E-4	0.0	0.0
7	0.0	0.0	0.002519357060078268	0.0	0.0
8	0.0	0.0	0.003359142746771024	0.0	0.0
9	0.0	0.0	0.003918999871232861	8.39785686692756E-4	0.0
10	0.0	0.0	0.003918999871232861	8.39785686692756E-4	0.0
11	0.0	0.0	0.006438356931311129	8.39785686692756E-4	0.0
12	0.0	0.0	0.006998214055772967	8.39785686692756E-4	0.0
13	0.0	0.0	0.0072781426180038855	8.39785686692756E-4	0.0
14	0.0	0.0	0.007837999742465723	8.39785686692756E-4	0.0
15	0.0	0.0	0.00839785686692756	8.39785686692756E-4	0.0
16	0.0	0.0	0.010077428240313073	8.39785686692756E-4	0.0
17	0.0	0.0	0.01063728536477491	8.39785686692756E-4	0.0
18	0.0	0.0	0.010917213927005828	8.39785686692756E-4	0.0
19	0.0	0.0	0.010917213927005828	0.0011197142489236746	0.0
20	0.0	0.0	0.010917213927005828	0.0011197142489236746	0.0
21	0.0	0.0	0.011197142489236747	0.001679571373385512	0.0
22	0.0	0.0	0.011197142489236747	0.001679571373385512	0.0
23	2.7992856223091865E-4	0.0	0.011477071051467665	0.0019594999356164306	0.0
24	2.7992856223091865E-4	0.0	0.011477071051467665	0.002239428497847349	0.0
25	2.7992856223091865E-4	0.0	0.011477071051467665	0.002519357060078268	0.0
26	2.7992856223091865E-4	0.0	0.011477071051467665	0.0027992856223091867	0.0
27	2.7992856223091865E-4	0.0	0.011477071051467665	0.003359142746771024	0.0
28	2.7992856223091865E-4	0.0	0.011477071051467665	0.007837999742465723	0.0
29	2.7992856223091865E-4	0.0	0.011477071051467665	0.017915427982778794	0.0
30	2.7992856223091865E-4	0.0	0.011477071051467665	0.03695057021448126	0.0
31	2.7992856223091865E-4	0.0	0.011477071051467665	0.09657535396966695	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTTCG	30	3.5945565E-4	30.833334	21
TTCGCCG	30	3.5945565E-4	30.833334	24
GGTATCA	210	0.0	30.833332	1
GCCGCTC	40	0.0019285965	23.125	27
TCCAGCG	40	0.0019285965	23.125	2
CCGCTCT	50	2.6970534E-4	22.199999	28
CCTAGTC	45	0.0038207255	20.555555	2
CGCCGCT	45	0.0038207255	20.555555	26
TAAGAGT	45	0.0038207255	20.555555	4
TAACTCC	55	5.133562E-4	20.181818	4
GTATCAA	545	0.0	20.027525	1
GTTTAGG	75	9.236486E-6	19.733334	1
CGTTGAA	60	9.219835E-4	18.5	16
GCCTGTT	70	1.2162147E-4	18.5	11
TACTTAA	50	0.0070256256	18.499998	7
CGCCTGT	50	0.0070256256	18.499998	10
TATAGTG	100	2.8611612E-7	18.499998	5
GGGGTTA	115	6.368282E-8	17.695652	6
TTTAGGA	95	3.5923294E-6	17.526316	2
ACAGTGG	85	2.7146185E-5	17.411764	8
>>END_MODULE