FastQCFastQC Report
Fri 10 Feb 2017
ERR1632764.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632764.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324348
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9610.29628670440391186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.2182840652632358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4020.12394095231048133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1950.031.307691
GCTTACA250.005490598729.61
ATCTGCG400.001928215123.1250027
TAATACG502.69631E-422.22
GTATCAA5600.022.133931
TATACTG603.715678E-521.5833345
TCTATAC656.882623E-519.9230773
TTAGACT500.00702426218.54
TATATAC500.00702426218.53
TCGGCAA701.2157757E-418.533
ACCTATA500.00702426218.51
TCTAATA1103.8262442E-818.52
GTGTATA609.217338E-418.51
ACCCGCA500.00702426218.526
ACGAACC500.00702426218.526
AGGTCGC701.2157757E-418.535
CGGCAAA752.0615068E-417.26666634
TAGATGT752.0615068E-417.2666664
GTATAAA752.0615068E-417.2666661
TAATAGT650.0015766317.0769234
Produced by FastQC (version 0.11.5)