FastQCFastQC Report
Fri 10 Feb 2017
ERR1632763.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632763.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264408
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9910.37479955220719496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.25831291035067017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3710.1403134549635412No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2830.1070315572902484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCA200.00183937437.033
ATCTATA250.00548878829.5999982
TACGCAT250.00548878829.59999834
GTCTTAT250.00548878829.5999981
CTAGGTC250.00548878829.5999984
GGGGCGA250.00548878829.59999830
TCTATTC405.9231068E-527.7500023
GGTATCA2200.026.909091
ATTAACG358.8512676E-426.42857212
GTATCAA6600.024.9469721
CGATTAA400.001927277723.12500210
GATTAAC502.694485E-422.19999911
GAGTAAT603.7123347E-521.5833341
ATATTAT603.7123347E-521.5833341
CCGATTA450.00381813720.5555579
AATGTAC450.00381813720.5555573
ATGCTCA902.1394917E-618.532
GTCAACC701.2146973E-418.515
TGAAGTT500.007020915418.4999985
GTAATCC500.007020915418.4999983
Produced by FastQC (version 0.11.5)