##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632763.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	264408
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68856842455599	33.0	33.0	33.0	27.0	33.0
2	31.220000907688117	33.0	33.0	33.0	27.0	33.0
3	31.65283576896312	33.0	33.0	33.0	27.0	33.0
4	31.79869746755015	33.0	33.0	33.0	27.0	33.0
5	31.900604369005478	33.0	33.0	33.0	33.0	33.0
6	34.760366554718466	37.0	37.0	37.0	27.0	37.0
7	34.9325360806027	37.0	37.0	37.0	27.0	37.0
8	35.08810626002239	37.0	37.0	37.0	33.0	37.0
9	35.19010771232338	37.0	37.0	37.0	33.0	37.0
10	35.207160902847114	37.0	37.0	37.0	33.0	37.0
11	35.24078696559862	37.0	37.0	37.0	33.0	37.0
12	35.17164382318236	37.0	37.0	37.0	33.0	37.0
13	35.204774439502586	37.0	37.0	37.0	33.0	37.0
14	35.20765634927839	37.0	37.0	37.0	33.0	37.0
15	35.21727784333303	37.0	37.0	37.0	33.0	37.0
16	35.22550754893952	37.0	37.0	37.0	33.0	37.0
17	35.18113294605307	37.0	37.0	37.0	33.0	37.0
18	35.19123097636985	37.0	37.0	37.0	33.0	37.0
19	35.19301987836979	37.0	37.0	37.0	33.0	37.0
20	35.211877099028776	37.0	37.0	37.0	33.0	37.0
21	35.20816692384496	37.0	37.0	37.0	33.0	37.0
22	35.20641584218329	37.0	37.0	37.0	33.0	37.0
23	35.194483525460655	37.0	37.0	37.0	33.0	37.0
24	35.19957036095731	37.0	37.0	37.0	33.0	37.0
25	35.18250582433209	37.0	37.0	37.0	33.0	37.0
26	35.135472451665606	37.0	37.0	37.0	33.0	37.0
27	35.08911984508789	37.0	37.0	37.0	33.0	37.0
28	35.05010438413361	37.0	37.0	37.0	27.0	37.0
29	35.08715696953193	37.0	37.0	37.0	33.0	37.0
30	35.050936431575444	37.0	37.0	37.0	27.0	37.0
31	35.03420471392696	37.0	37.0	37.0	27.0	37.0
32	35.067059241777855	37.0	37.0	37.0	27.0	37.0
33	35.05618211249281	37.0	37.0	37.0	27.0	37.0
34	34.98721294363257	37.0	37.0	37.0	27.0	37.0
35	34.97423678557381	37.0	37.0	37.0	27.0	37.0
36	34.93912438352849	37.0	37.0	37.0	27.0	37.0
37	34.99240189404254	37.0	37.0	37.0	27.0	37.0
38	34.842909442982055	37.0	37.0	37.0	27.0	37.0
39	34.917275574112736	37.0	37.0	37.0	27.0	37.0
40	34.95421848053009	37.0	37.0	37.0	27.0	37.0
41	34.9590708299295	37.0	37.0	37.0	27.0	37.0
42	34.87166802819885	37.0	37.0	37.0	27.0	37.0
43	34.663580527064234	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	6.0
17	21.0
18	11.0
19	15.0
20	19.0
21	57.0
22	186.0
23	362.0
24	804.0
25	1395.0
26	2266.0
27	3461.0
28	4803.0
29	6404.0
30	8467.0
31	10650.0
32	13526.0
33	17536.0
34	24512.0
35	44391.0
36	125509.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61712202353938	19.863241656833377	13.4326495416175	25.08698677800974
2	16.18029711657741	22.69447217935917	37.94438897461499	23.180841729448428
3	18.212383891561526	26.448518955553542	30.013842243805026	25.32525490907991
4	12.787812774197452	17.7551360019364	38.78891712807479	30.668134095791356
5	14.436401319173397	36.459940697709605	34.81286496626426	14.290793016852742
6	30.002117938942845	39.33050437203111	17.265362621403284	13.402015067622767
7	27.078983994432846	31.880275937187978	22.96677861486793	18.073961453511238
8	24.814680342500985	35.53069498653596	20.342047139269614	19.312577531693442
9	26.247692959365825	15.329339505612538	19.837523826813108	38.58544370820853
10	15.196590118302018	27.792653777495385	33.70699827539257	23.30375782881002
11	34.70961544280052	23.374103657983117	23.720916159874132	18.195364739342228
12	23.267072100692868	25.154306980121632	30.26307827297207	21.31554264621343
13	28.624323015945052	21.999712565429185	25.658830292578138	23.717134126047622
14	22.333666152310066	20.507322017488125	27.53509727391002	29.62391455629179
15	24.180433269795166	28.218132582977823	24.272336691779373	23.32909745544764
16	23.244379897733804	27.94960818129557	25.051435660040543	23.754576260930076
17	22.69711960303773	27.10659284136637	26.168270249009108	24.02801730658679
18	23.810550361562434	26.532858309884723	27.142900366100875	22.513690962451967
19	25.264742367855735	26.058213064657647	26.697754984720586	21.97928958276603
20	24.761731868929836	25.789310459592752	26.77453027139875	22.674427400078667
21	23.70919185501195	26.43149980333424	27.01620223291277	22.843106108741036
22	23.30337962542737	26.52869807267556	26.799869898036366	23.368052403860702
23	23.30678345587123	26.350942482829566	27.210976974978063	23.13129708632114
24	23.322667997942574	26.560088953435603	26.373256482405978	23.74398656621585
25	23.612749992435933	26.53966597077244	27.072932740310428	22.774651296481196
26	23.723563583552693	26.63156939275665	26.798735287888416	22.846131735802246
27	23.700114973828327	26.485204683670695	26.666742307343196	23.147938035157786
28	23.225469728601254	26.506762276481798	27.033221385132066	23.23454660978488
29	23.934601071071977	26.41183322743639	26.568787631236574	23.08477807025506
30	23.71448670236907	26.282487670569726	26.999939487458775	23.003086139602434
31	23.65851260173671	26.11910380926447	26.77074823757224	23.451635351426585
32	22.703549060542798	26.221218722580254	27.29796375298781	23.777268463889143
33	23.058303833469488	26.265468518350428	27.52866781640495	23.147559831775137
34	23.19521345798917	26.08355269129527	27.16559256905994	23.555641281655625
35	23.161931560315878	26.819914677316874	26.953420471392697	23.064733290974555
36	23.82719131039908	26.250340383044385	26.744652204169313	23.17781610238722
37	23.453526368339837	26.237859671416903	26.839959456597377	23.46865450364588
38	23.509122265589543	26.40124353272216	26.76545339021512	23.324180811473177
39	23.166470000907687	26.22953919699858	27.052509757647275	23.55148104444646
40	23.164957187377084	26.64253729085353	27.229887144110617	22.96261837765877
41	23.383558742549393	25.798765544159025	27.15878490817222	23.658890805119363
42	22.979637529878065	26.506005869716496	27.12323379020302	23.391122810202415
43	23.45806480893165	25.700810868052404	27.19131039908021	23.649813923935735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	8.0
2	11.0
3	53.5
4	96.0
5	96.0
6	122.0
7	148.0
8	139.5
9	131.0
10	185.0
11	239.0
12	239.0
13	369.5
14	500.0
15	865.5
16	1231.0
17	1454.0
18	1677.0
19	1677.0
20	1534.5
21	1392.0
22	1564.5
23	1737.0
24	2331.5
25	2926.0
26	2926.0
27	3845.0
28	4764.0
29	5834.5
30	6905.0
31	8302.5
32	9700.0
33	9700.0
34	11239.5
35	12779.0
36	13999.0
37	15219.0
38	16590.0
39	17961.0
40	17961.0
41	18861.5
42	19762.0
43	20229.0
44	20696.0
45	20902.0
46	21108.0
47	21108.0
48	20773.0
49	20438.0
50	20318.0
51	20198.0
52	19321.0
53	18444.0
54	18444.0
55	17565.5
56	16687.0
57	15033.5
58	13380.0
59	12337.0
60	11294.0
61	11294.0
62	9847.0
63	8400.0
64	6968.5
65	5537.0
66	4796.5
67	4056.0
68	4056.0
69	3403.0
70	2750.0
71	2258.0
72	1766.0
73	1428.5
74	1091.0
75	1091.0
76	856.5
77	622.0
78	478.5
79	335.0
80	257.0
81	179.0
82	179.0
83	143.5
84	108.0
85	93.0
86	78.0
87	59.0
88	40.0
89	40.0
90	27.0
91	14.0
92	9.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	264408.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.90022614238421
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65558447354283	64.46256798575361
2	5.9805420323717735	8.600046491631293
3	1.588402660856718	3.4261953156228855
4	0.733329909167032	2.109063452243347
5	0.4273464989904	1.5363154959282965
6	0.2998575298530269	1.2935894524157616
7	0.22328093671723287	1.1237764890276696
8	0.1544184438650584	0.8882176827562208
9	0.11808302798090183	0.7641176773863891
>10	0.7404271849085392	9.881066122150415
>50	0.053365486418732525	2.6938712803199594
>100	0.024305073022393035	2.5852198649805844
>500	0.0010567423053214364	0.6359526897836147
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	991	0.37479955220719496	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	683	0.25831291035067017	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	371	0.1403134549635412	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	283	0.1070315572902484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	7.564067653021088E-4	0.0	0.0
2	0.0	0.0	7.564067653021088E-4	0.0	0.0
3	0.0	0.0	0.0011346101479531633	0.0	0.0
4	0.0	0.0	0.0011346101479531633	0.0	0.0
5	0.0	0.0	0.0011346101479531633	0.0	0.0
6	0.0	0.0	0.002647423678557381	0.0	0.0
7	0.0	0.0	0.004916643974463708	0.0	0.0
8	0.0	0.0	0.006429457505067925	0.0	0.0
9	0.0	0.0	0.007564067653021088	3.782033826510544E-4	0.0
10	3.782033826510544E-4	0.0	0.008320474418323197	3.782033826510544E-4	0.0
11	3.782033826510544E-4	0.0	0.011724304862182688	3.782033826510544E-4	0.0
12	3.782033826510544E-4	0.0	0.012480711627484797	3.782033826510544E-4	0.0
13	3.782033826510544E-4	0.0	0.01361532177543796	3.782033826510544E-4	0.0
14	3.782033826510544E-4	0.0	0.015128135306042177	3.782033826510544E-4	0.0
15	3.782033826510544E-4	0.0	0.01701915221929745	3.782033826510544E-4	0.0
16	3.782033826510544E-4	0.0	0.01777555898459956	3.782033826510544E-4	0.0
17	3.782033826510544E-4	0.0	0.018153762367250612	7.564067653021088E-4	0.0
18	3.782033826510544E-4	0.0	0.018153762367250612	7.564067653021088E-4	0.0
19	3.782033826510544E-4	0.0	0.018153762367250612	7.564067653021088E-4	0.0
20	3.782033826510544E-4	0.0	0.018910169132552723	7.564067653021088E-4	0.0
21	3.782033826510544E-4	0.0	0.020044779280505885	7.564067653021088E-4	0.0
22	3.782033826510544E-4	0.0	0.02042298266315694	7.564067653021088E-4	0.0
23	3.782033826510544E-4	0.0	0.020801186045807992	0.0011346101479531633	0.0
24	3.782033826510544E-4	0.0	0.020801186045807992	0.0015128135306042177	0.0
25	3.782033826510544E-4	0.0	0.020801186045807992	0.0022692202959063265	0.0
26	3.782033826510544E-4	0.0	0.020801186045807992	0.0022692202959063265	0.0
27	3.782033826510544E-4	0.0	0.020801186045807992	0.00340383044385949	0.0
28	3.782033826510544E-4	0.0	0.020801186045807992	0.01021149133157847	0.0
29	3.782033826510544E-4	0.0	0.020801186045807992	0.022692202959063264	0.0
30	3.782033826510544E-4	0.0	0.020801186045807992	0.04538440591812653	0.0
31	3.782033826510544E-4	0.0	0.020801186045807992	0.11383921817796738	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCA	20	0.001839374	37.0	33
ATCTATA	25	0.005488788	29.599998	2
TACGCAT	25	0.005488788	29.599998	34
GTCTTAT	25	0.005488788	29.599998	1
CTAGGTC	25	0.005488788	29.599998	4
GGGGCGA	25	0.005488788	29.599998	30
TCTATTC	40	5.9231068E-5	27.750002	3
GGTATCA	220	0.0	26.90909	1
ATTAACG	35	8.8512676E-4	26.428572	12
GTATCAA	660	0.0	24.946972	1
CGATTAA	40	0.0019272777	23.125002	10
GATTAAC	50	2.694485E-4	22.199999	11
GAGTAAT	60	3.7123347E-5	21.583334	1
ATATTAT	60	3.7123347E-5	21.583334	1
CCGATTA	45	0.003818137	20.555557	9
AATGTAC	45	0.003818137	20.555557	3
ATGCTCA	90	2.1394917E-6	18.5	32
GTCAACC	70	1.2146973E-4	18.5	15
TGAAGTT	50	0.0070209154	18.499998	5
GTAATCC	50	0.0070209154	18.499998	3
>>END_MODULE