FastQCFastQC Report
Fri 10 Feb 2017
ERR1632762.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632762.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347117
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8150.2347911511104325No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7240.20857520663061735No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7070.2036777224970255No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6680.19244231771996187No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6440.18552822247253808No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5880.16939533356188258No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5490.15815992878481894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.15412670655715507No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5250.15124583353739515No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5100.14692452400775532No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5030.14490791289392338No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG4460.12848693668129188No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4420.12733458747338794No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4350.125317976359556No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT4260.12272519064177208No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4260.12272519064177208No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4150.11955623032003618No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4150.11955623032003618No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4100.11811579381015623No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT3970.11437065888446835No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC3900.11235404777063641No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA3790.10918508744890051No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3710.10688038903309259No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG3700.1065923017311166No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT3640.10486377791926066No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG3550.10227099220147674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAC200.001840166337.03
CGGGATT303.5943498E-430.83333217
GGTATCA2000.024.9751
TTGCGGT451.320614E-424.66666835
TAGTACC1150.024.1304364
TCTAGCA851.9645086E-923.9411773
GTATCAA6000.022.5083331
AGTACCG1201.0913936E-1121.5833325
CGCAAGG1151.3460522E-1020.91304410
CCGCAAG1151.3460522E-1020.9130449
GTATTAG2050.020.7560961
TCACCCG450.003820509920.55555727
AACTAGA1650.020.1818227
ACGTGAT555.13316E-420.18181837
AGGTCGC1403.6379788E-1219.8214335
TAATAGT759.235251E-619.7333344
CACATGT1500.019.73333428
ATAGTAC1600.019.656253
CTCTAGC851.2401906E-619.5882362
ACCAGTA951.667704E-719.47368412
Produced by FastQC (version 0.11.5)