##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632762.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347117
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.726351633599045	33.0	33.0	33.0	27.0	33.0
2	31.240970623737816	33.0	33.0	33.0	27.0	33.0
3	31.672974818288935	33.0	33.0	33.0	27.0	33.0
4	31.82085003039321	33.0	33.0	33.0	27.0	33.0
5	31.920268958305126	33.0	33.0	33.0	33.0	33.0
6	34.81768107007147	37.0	37.0	37.0	27.0	37.0
7	34.96536902542947	37.0	37.0	37.0	27.0	37.0
8	35.13369843597404	37.0	37.0	37.0	33.0	37.0
9	35.23098263697831	37.0	37.0	37.0	33.0	37.0
10	35.23176623443968	37.0	37.0	37.0	33.0	37.0
11	35.2738154570361	37.0	37.0	37.0	33.0	37.0
12	35.22176096244206	37.0	37.0	37.0	33.0	37.0
13	35.254337874549506	37.0	37.0	37.0	33.0	37.0
14	35.234647107459445	37.0	37.0	37.0	33.0	37.0
15	35.25725619891852	37.0	37.0	37.0	33.0	37.0
16	35.250846256449556	37.0	37.0	37.0	33.0	37.0
17	35.211268822904096	37.0	37.0	37.0	33.0	37.0
18	35.23288401317135	37.0	37.0	37.0	33.0	37.0
19	35.2219568618074	37.0	37.0	37.0	33.0	37.0
20	35.216633008466886	37.0	37.0	37.0	33.0	37.0
21	35.230461198961734	37.0	37.0	37.0	33.0	37.0
22	35.24188962223112	37.0	37.0	37.0	33.0	37.0
23	35.2296689588813	37.0	37.0	37.0	33.0	37.0
24	35.20748911750217	37.0	37.0	37.0	33.0	37.0
25	35.20921764131403	37.0	37.0	37.0	33.0	37.0
26	35.18321776231069	37.0	37.0	37.0	33.0	37.0
27	35.11920764468464	37.0	37.0	37.0	33.0	37.0
28	35.09394526917437	37.0	37.0	37.0	33.0	37.0
29	35.13368115073592	37.0	37.0	37.0	33.0	37.0
30	35.08040228510848	37.0	37.0	37.0	33.0	37.0
31	35.085403480670784	37.0	37.0	37.0	27.0	37.0
32	35.09946214100721	37.0	37.0	37.0	33.0	37.0
33	35.09695866235304	37.0	37.0	37.0	33.0	37.0
34	35.03406632345866	37.0	37.0	37.0	27.0	37.0
35	35.03442643258613	37.0	37.0	37.0	27.0	37.0
36	35.013269301129014	37.0	37.0	37.0	27.0	37.0
37	35.055834199995964	37.0	37.0	37.0	27.0	37.0
38	34.89971104843612	37.0	37.0	37.0	27.0	37.0
39	34.97407214282216	37.0	37.0	37.0	27.0	37.0
40	34.99412878078573	37.0	37.0	37.0	27.0	37.0
41	35.034527263141825	37.0	37.0	37.0	27.0	37.0
42	34.949538628185884	37.0	37.0	37.0	27.0	37.0
43	34.744031551321314	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	6.0
16	23.0
17	18.0
18	11.0
19	14.0
20	23.0
21	59.0
22	196.0
23	481.0
24	1003.0
25	1771.0
26	2934.0
27	4382.0
28	6160.0
29	8239.0
30	10544.0
31	13681.0
32	17277.0
33	22708.0
34	31984.0
35	58748.0
36	166852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.31464895121818	21.36714710025726	13.433510891140452	24.884693057384112
2	16.078440410582022	22.595551355882886	38.26663632147086	23.059371912064233
3	16.928586038713174	27.270632092349267	30.33213585044812	25.468646018489444
4	12.148641524327532	18.24744970715926	40.15908180815114	29.444826960362068
5	13.190365208272715	37.90566293209494	36.08495118360668	12.819020676025664
6	29.224440174350434	40.75340591212761	17.401049213953794	12.621104699568155
7	26.700219234436805	32.23524056730152	24.132785199226774	16.931754999034908
8	23.77670929398445	37.216846193070346	20.298919384530286	18.707525128414915
9	25.300114946833492	15.936701458009836	20.94135406793675	37.82182952721993
10	14.99292745673649	28.14756983956418	34.281236585935005	22.57826611776433
11	33.84651284725323	23.965118389476746	24.356052858258167	17.832315905011857
12	22.908702253130787	26.332043662511488	30.035117842110875	20.724136242246853
13	26.78174794089601	22.677368149644067	26.40377740070351	24.137106508756414
14	21.33574558434188	21.147912663453532	27.946196815482967	29.570144936721626
15	23.590316809605984	28.957383245418693	24.874898089116925	22.5774018558584
16	22.84906818162176	29.127066666282552	25.15924025616723	22.86462489592846
17	22.183298426755243	28.190782934860582	26.542347392953964	23.083571245430214
18	22.214699942670627	28.43738566535203	27.9410112440474	21.40690314792995
19	24.424617636128453	26.805659186960018	27.34985610039266	21.419867076518866
20	23.481995984063012	27.022877012649914	27.916523823379435	21.578603179907642
21	22.417513403261722	27.75404258506498	27.837299815336042	21.99114419633726
22	22.19597426804219	27.585799600711	27.585511513409024	22.63271461783779
23	22.37602883177718	27.239518663735858	28.386970387506228	21.99748211698073
24	22.812481094270808	28.2913254032502	25.91114811432457	22.985045388154425
25	22.61312468130342	27.731283688208876	27.674818577021583	21.98077305346612
26	22.62925757021408	27.897798148750997	27.739926307268153	21.73301797376677
27	22.357303157148742	27.97241275996278	26.562513504092276	23.1077705787962
28	21.947066839134934	27.261701385988008	28.287580268324515	22.503651506552547
29	24.124430667469472	27.16663257633593	26.807963885375823	21.900972870818773
30	23.225886372606354	26.761869917059666	27.639671926180508	22.372571784153468
31	22.64769515754054	27.258820512968253	27.609710846775005	22.483773482716202
32	21.75520069601892	26.9834090522792	28.012456894937444	23.248933356764432
33	21.6618604101787	27.223385774825203	28.540520919459432	22.574232895536664
34	22.938375245234315	26.744296591639134	28.184156926915133	22.13317123621142
35	22.69782234808436	27.91277868845375	27.26054903678011	22.12884992668178
36	23.965118389476746	27.192560433513773	26.66824154391747	22.17407963309201
37	22.341170268238088	26.769072099609065	27.459617362445517	23.43014026970733
38	22.807583610137215	27.31845458447728	27.413523394129356	22.46043841125615
39	22.079010823439933	27.304050219378478	27.810795783554248	22.806143173627337
40	22.263098609402594	28.14036765701478	27.90039093446878	21.696142799113844
41	22.27692679989744	26.597948242235326	27.8833937836522	23.241731174215033
42	21.97731600584241	27.947637251992845	27.64111236269039	22.433934379474355
43	23.21551522973522	26.098692947910934	27.581766378483337	23.10402544387051
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	12.5
2	19.0
3	50.5
4	82.0
5	82.0
6	97.0
7	112.0
8	118.0
9	124.0
10	151.0
11	178.0
12	178.0
13	286.5
14	395.0
15	705.0
16	1015.0
17	1269.5
18	1524.0
19	1524.0
20	1510.0
21	1496.0
22	1806.5
23	2117.0
24	3040.0
25	3963.0
26	3963.0
27	5388.0
28	6813.0
29	8486.5
30	10160.0
31	12905.5
32	15651.0
33	15651.0
34	18115.5
35	20580.0
36	22966.0
37	25352.0
38	27056.0
39	28760.0
40	28760.0
41	30195.0
42	31630.0
43	31839.5
44	32049.0
45	30499.0
46	28949.0
47	28949.0
48	26891.5
49	24834.0
50	24515.5
51	24197.0
52	22219.0
53	20241.0
54	20241.0
55	19949.5
56	19658.0
57	16262.5
58	12867.0
59	12025.0
60	11183.0
61	11183.0
62	9870.0
63	8557.0
64	6895.0
65	5233.0
66	4333.5
67	3434.0
68	3434.0
69	2884.0
70	2334.0
71	1914.5
72	1495.0
73	1192.5
74	890.0
75	890.0
76	715.5
77	541.0
78	402.0
79	263.0
80	209.5
81	156.0
82	156.0
83	124.5
84	93.0
85	93.5
86	94.0
87	72.0
88	50.0
89	50.0
90	34.0
91	18.0
92	10.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	347117.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.80622153025015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.89555020361254	53.165069685307266
2	6.019519309540057	7.200094106639423
3	1.6526856465598379	2.965226516940671
4	0.8111007699659775	1.9403548932776686
5	0.4946251935901846	1.4790831951148724
6	0.34135918601151133	1.224924185999419
7	0.23775395998913368	0.9953416200563052
8	0.1836464987606287	0.8786562550506376
9	0.12633634354367967	0.6800129414365585
>10	1.0112257554621327	11.901679423254418
>50	0.12620440895004484	5.179828966455745
>100	0.09465330671253308	10.424975509409375
>500	0.005339417301732635	1.9647527010576884
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	815	0.2347911511104325	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	724	0.20857520663061735	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	707	0.2036777224970255	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	668	0.19244231771996187	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	644	0.18552822247253808	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	588	0.16939533356188258	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	549	0.15815992878481894	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	535	0.15412670655715507	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	525	0.15124583353739515	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	510	0.14692452400775532	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	503	0.14490791289392338	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	446	0.12848693668129188	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	442	0.12733458747338794	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	435	0.125317976359556	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT	426	0.12272519064177208	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	426	0.12272519064177208	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	415	0.11955623032003618	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	415	0.11955623032003618	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	410	0.11811579381015623	No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT	397	0.11437065888446835	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	390	0.11235404777063641	No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA	379	0.10918508744890051	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	371	0.10688038903309259	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	370	0.1065923017311166	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	364	0.10486377791926066	No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG	355	0.10227099220147674	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.880873019759908E-4	0.0	0.0
2	0.0	0.0	2.880873019759908E-4	0.0	0.0
3	0.0	0.0	2.880873019759908E-4	0.0	0.0
4	0.0	0.0	0.0011523492079039631	0.0	0.0
5	0.0	0.0	0.0011523492079039631	0.0	0.0
6	0.0	0.0	0.001728523811855945	0.0	0.0
7	0.0	0.0	0.002592785717783917	2.880873019759908E-4	0.0
8	0.0	0.0	0.002880873019759908	2.880873019759908E-4	0.0
9	2.880873019759908E-4	0.0	0.002880873019759908	5.761746039519816E-4	0.0
10	2.880873019759908E-4	0.0	0.002880873019759908	5.761746039519816E-4	0.0
11	2.880873019759908E-4	0.0	0.0046093968316158525	8.642619059279725E-4	0.0
12	2.880873019759908E-4	0.0	0.0046093968316158525	0.0011523492079039631	0.0
13	2.880873019759908E-4	0.0	0.0046093968316158525	0.0011523492079039631	0.0
14	2.880873019759908E-4	0.0	0.005473658737543826	0.0011523492079039631	0.0
15	2.880873019759908E-4	0.0	0.006049833341495807	0.0011523492079039631	0.0
16	2.880873019759908E-4	0.0	0.006337920643471798	0.0011523492079039631	0.0
17	2.880873019759908E-4	0.0	0.006337920643471798	0.0011523492079039631	0.0
18	2.880873019759908E-4	0.0	0.006626007945447788	0.0011523492079039631	0.0
19	2.880873019759908E-4	0.0	0.00720218254939977	0.0011523492079039631	0.0
20	2.880873019759908E-4	0.0	0.00720218254939977	0.0011523492079039631	0.0
21	2.880873019759908E-4	0.0	0.007490269851375761	0.0011523492079039631	0.0
22	2.880873019759908E-4	0.0	0.007778357153351752	0.0011523492079039631	0.0
23	2.880873019759908E-4	0.0	0.007778357153351752	0.0011523492079039631	0.0
24	2.880873019759908E-4	0.0	0.007778357153351752	0.001728523811855945	0.0
25	2.880873019759908E-4	0.0	0.007778357153351752	0.001728523811855945	0.0
26	2.880873019759908E-4	0.0	0.007778357153351752	0.001728523811855945	0.0
27	2.880873019759908E-4	0.0	0.007778357153351752	0.002592785717783917	0.0
28	2.880873019759908E-4	0.0	0.007778357153351752	0.008642619059279724	0.0
29	2.880873019759908E-4	0.0	0.007778357153351752	0.01814950002448742	0.0
30	2.880873019759908E-4	0.0	0.007778357153351752	0.034282388935142905	0.0
31	2.880873019759908E-4	0.0	0.007778357153351752	0.08296914296908535	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAC	20	0.0018401663	37.0	3
CGGGATT	30	3.5943498E-4	30.833332	17
GGTATCA	200	0.0	24.975	1
TTGCGGT	45	1.320614E-4	24.666668	35
TAGTACC	115	0.0	24.130436	4
TCTAGCA	85	1.9645086E-9	23.941177	3
GTATCAA	600	0.0	22.508333	1
AGTACCG	120	1.0913936E-11	21.583332	5
CGCAAGG	115	1.3460522E-10	20.913044	10
CCGCAAG	115	1.3460522E-10	20.913044	9
GTATTAG	205	0.0	20.756096	1
TCACCCG	45	0.0038205099	20.555557	27
AACTAGA	165	0.0	20.18182	27
ACGTGAT	55	5.13316E-4	20.181818	37
AGGTCGC	140	3.6379788E-12	19.82143	35
TAATAGT	75	9.235251E-6	19.733334	4
CACATGT	150	0.0	19.733334	28
ATAGTAC	160	0.0	19.65625	3
CTCTAGC	85	1.2401906E-6	19.588236	2
ACCAGTA	95	1.667704E-7	19.473684	12
>>END_MODULE