FastQCFastQC Report
Fri 10 Feb 2017
ERR1632761.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632761.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369514
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9510.25736507953690524No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7100.19214427599495554No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6440.17428297709964982No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6370.17238859691378405No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6360.17211797117294608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.1523622920917746No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5370.1453260228299875No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4490.12151095763624653No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA4440.1201578289320567No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT4400.11907532596870485No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4400.11907532596870485No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4400.11907532596870485No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4250.11501593985613537No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA3920.1060852904084825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACGC200.001840320537.023
ACACGAT352.3839562E-531.71428515
TTAGACT250.005491576629.64
CTACACT604.3060936E-827.7500024
TCGCCAT1250.026.6413
GTCGCCA1250.026.6412
TAGGGGT451.3208395E-424.6666684
GTATCAA5450.023.7614671
TCACACG551.8981327E-523.54545413
GGTATCA2150.023.2325571
TTCAACG400.001928721823.12519
ACGATAA400.001928721823.12517
GTTCGTT400.001928721823.12510
GGTTCGT400.001928721823.1259
CGTTTGT400.001928721823.12513
TCAACGA400.001928721823.12520
TCTATCC400.001928721823.1253
CTTACCG502.697296E-422.29
ATCCCTT855.156835E-821.7647085
TTGGCCG1550.021.48387131
Produced by FastQC (version 0.11.5)