FastQCFastQC Report
Fri 10 Feb 2017
ERR1632760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412416
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21460.5203483860955929No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC17620.42723851644941035No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC16130.39110994723774056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15870.3848056331471136No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG14690.3561937461204221No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG14260.3457673805090006No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC13810.3348560676598386No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA13690.3319463842333954No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC13470.32661196461824954No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG13050.31642807262569833No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT12950.31400333643699563No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG12780.30988128491620115No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA11990.29072586902545006No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT11550.2800570297951583No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA11370.2756925046554935No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10980.26623603351955305No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10390.25193009000620736No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT10370.2514451427684668No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC10270.24902040657976415No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG10070.24417093420235877No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC9460.22938004345127253No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT9430.2286526225946617No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG9100.22065099317194287No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG8960.21725636250775915No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC8590.2082848386095593No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA8350.20246547175667284No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC8210.19907084109248913No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG8210.19907084109248913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8130.19713105214152699No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT8040.1949487895716946No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG8030.1947063159528243No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC7950.1927665270018622No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA7510.18209768777157045No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT7400.17943047796399753No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA7150.17336863749224082No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT7120.17264121663563003No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT7110.17239874301675978No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC6940.16827669149596525No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT6870.16657937616387336No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA6730.16318474549968964No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGC6700.16245732464307883No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTAAGATTTG6630.16076000931098697No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGA6510.15785032588454376No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT6470.15688043140906271No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG6420.15566806331471136No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCC6340.15372827436374922No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATG6300.15275837988826815No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTT6220.150818590937306No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG6220.150818590937306No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCAT6090.14766643389199255No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT6090.14766643389199255No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC6070.14718148665425201No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC6070.14718148665425201No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6060.14693901303538176No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTAC6040.14645406579764123No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTAT6030.14621159217877094No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAG6030.14621159217877094No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTA5990.14524169770328987No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCAT5870.1423320142768467No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG5810.14087717256362509No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5790.14039222532588455No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG5690.13796748913718188No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGT5580.13530027932960895No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGG5410.13117822780881438No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGAT5350.12972338609559278No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA5320.128995965238982No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5310.12875349162011174No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAA5240.12705617628801985No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTAC5200.12608628181253878No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG5010.12147928305400373No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATT4880.11832712600869026No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT4810.11662981067659839No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAAT4800.11638733705772812No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA4740.11493249534450653No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA4700.11396260086902546No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGA4590.1112953910614525No Hit
GATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGAT4560.1105679702048417No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGC4560.1105679702048417No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT4490.10887065487274984No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC4490.10887065487274984No Hit
AAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCT4460.10814323401613904No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGA4460.10814323401613904No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4450.10790076039726877No Hit
GGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCG4390.10644591868404717No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTT4350.10547602420856611No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCT4270.10353623525760397No Hit
AATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCC4250.10305128801986344No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG4240.10280881440099317No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCT4230.1025663407821229No Hit
GCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGG4230.1025663407821229No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA4200.10183891992551211No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT4200.10183891992551211No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTAC4150.10062655183116077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACCG200.001840567737.034
CCGCTCT601.2660166E-930.83333428
CGCTCTC601.2660166E-930.83333429
CCGGGGC250.005492308629.59999834
GGGCTTC250.005492308629.59999837
CGGGCGT250.005492308629.59999816
GGTATCA4600.029.3586961
CCGGCAG702.055458E-1029.07142816
TGTGACG405.931036E-527.75000210
CTACGGG405.931036E-527.75000211
AGGCAGC405.931036E-527.75000218
CAGTGGG405.931036E-527.75000224
GGAGGCA405.931036E-527.75000216
GCCGGCA754.6020432E-1027.13333315
GCCGCTC706.5665517E-926.4285727
GTGTGAC358.859715E-426.428579
CGGCAGC809.749783E-1025.43750217
CCGGCTA601.333934E-624.66666835
GTCCAGA451.3212032E-424.6666661
CGCCGGC901.4188117E-1024.66666614
Produced by FastQC (version 0.11.5)