FastQCFastQC Report
Fri 10 Feb 2017
ERR1632759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378156
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24450.6465585631326755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.4479632744158495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8950.23667481145347424No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6140.1623668538909868No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5390.14253376913231577No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5310.14041824009139087No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5220.13803826992035034No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4300.11370968594971388No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.11238748029913581No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG3910.10339648187520495No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3830.10128095283428004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT352.3841028E-531.7142856
AGGCCGT250.00549173829.66
GGTATCA3600.029.2916681
GTATCAA12400.025.5120981
CGTCCGC1300.024.19230829
CCGTCCG1350.023.29629728
GTCCGCC1350.023.29629730
CCCGTGC400.001928804423.1250028
CGCCGTC1500.022.226
GAGTCGC1350.021.92592622
TCCGGCG1450.021.68965516
CGAGTCG1400.021.14285721
TCCGCCG1500.020.96666731
CGCCTGG1500.020.96666736
GTCCGGC1700.020.67647215
GATCCGT450.003821132520.55555729
AAGTTAG450.003821132520.55555735
AGTTAGA555.1343284E-420.18181836
AGTCGCC1600.019.6562523
GCCGTCC1700.019.58823627
Produced by FastQC (version 0.11.5)