##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632759.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	378156
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68104168649975	33.0	33.0	33.0	27.0	33.0
2	31.213443658172817	33.0	33.0	33.0	27.0	33.0
3	31.65382276097695	33.0	33.0	33.0	27.0	33.0
4	31.774259829276808	33.0	33.0	33.0	27.0	33.0
5	31.894860851077333	33.0	33.0	33.0	33.0	33.0
6	34.75202033023408	37.0	37.0	37.0	27.0	37.0
7	34.8911137202636	37.0	37.0	37.0	27.0	37.0
8	35.10160885983562	37.0	37.0	37.0	33.0	37.0
9	35.14466516464105	37.0	37.0	37.0	33.0	37.0
10	35.209622483842644	37.0	37.0	37.0	33.0	37.0
11	35.23762415511059	37.0	37.0	37.0	33.0	37.0
12	35.14902844328795	37.0	37.0	37.0	33.0	37.0
13	35.2056188451327	37.0	37.0	37.0	33.0	37.0
14	35.156805656924654	37.0	37.0	37.0	33.0	37.0
15	35.21472884206518	37.0	37.0	37.0	33.0	37.0
16	35.21513343699426	37.0	37.0	37.0	33.0	37.0
17	35.17855329546536	37.0	37.0	37.0	33.0	37.0
18	35.16297771290155	37.0	37.0	37.0	33.0	37.0
19	35.17810374554416	37.0	37.0	37.0	33.0	37.0
20	35.12458879404267	37.0	37.0	37.0	33.0	37.0
21	35.17446503559378	37.0	37.0	37.0	33.0	37.0
22	35.200340600175586	37.0	37.0	37.0	33.0	37.0
23	35.1692529009192	37.0	37.0	37.0	33.0	37.0
24	35.16571732300955	37.0	37.0	37.0	33.0	37.0
25	35.17551222246903	37.0	37.0	37.0	33.0	37.0
26	35.1248849681084	37.0	37.0	37.0	33.0	37.0
27	35.05074625286919	37.0	37.0	37.0	33.0	37.0
28	35.040292894995716	37.0	37.0	37.0	27.0	37.0
29	35.08361099651995	37.0	37.0	37.0	33.0	37.0
30	35.03975343509028	37.0	37.0	37.0	27.0	37.0
31	35.029585673637335	37.0	37.0	37.0	27.0	37.0
32	35.06682162916891	37.0	37.0	37.0	27.0	37.0
33	35.02649964564888	37.0	37.0	37.0	27.0	37.0
34	34.96221400691778	37.0	37.0	37.0	27.0	37.0
35	34.9521916880864	37.0	37.0	37.0	27.0	37.0
36	34.93971535556754	37.0	37.0	37.0	27.0	37.0
37	34.96256835803214	37.0	37.0	37.0	27.0	37.0
38	34.796676503876704	37.0	37.0	37.0	27.0	37.0
39	34.86214419445943	37.0	37.0	37.0	27.0	37.0
40	34.8940992606226	37.0	37.0	37.0	27.0	37.0
41	34.908730788351896	37.0	37.0	37.0	27.0	37.0
42	34.761196437449094	37.0	37.0	37.0	27.0	37.0
43	34.57679634859687	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	12.0
16	16.0
17	18.0
18	18.0
19	15.0
20	11.0
21	68.0
22	226.0
23	544.0
24	1112.0
25	2025.0
26	3351.0
27	4884.0
28	7076.0
29	9444.0
30	12210.0
31	15205.0
32	19611.0
33	25429.0
34	36092.0
35	64504.0
36	176284.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1109277652609	19.74581918573287	13.794571552480987	24.34868149652524
2	16.065327536783762	23.157109764224288	38.20751224362432	22.570050455367628
3	18.290335205576536	27.087498281132653	30.17088185828071	24.451284655010102
4	12.482150223717197	18.094384328160864	39.37105321613302	30.05241223198891
5	14.581019473444822	36.18030654015803	34.36624038756492	14.872433598832227
6	28.841536297189517	40.12021493775056	17.18259131152223	13.855657453537692
7	26.472672653613856	32.669321655613025	22.622145358000402	18.23586033277272
8	24.58350522006791	35.228582912871936	20.153851849501265	20.03406001755889
9	26.62710627360137	15.04537809792784	19.6971620177916	38.630353610679194
10	15.385184950126401	27.697035086049144	33.057256793492634	23.86052317033182
11	34.69890732925036	23.80816382656893	23.44032621457811	18.0526026296026
12	23.56276245782164	25.521742349718107	29.736933963760986	21.178561228699266
13	29.021620706798252	22.138218089888827	25.67273823501412	23.167422968298798
14	22.669744761421214	20.897195866256254	27.67614423677001	28.756915135552525
15	24.665746411533863	27.698886173959952	24.758036365944214	22.87733104856197
16	22.618707623308897	28.193391087276147	25.21948613799596	23.96841515141899
17	22.45396079924687	26.769640042733688	26.00752070574049	24.768878452278955
18	24.25057383725235	25.581770486254353	27.42492516316018	22.74273051333312
19	25.943261511122394	25.449814362326663	27.020065792953176	21.586858333597775
20	25.35620220226573	25.027766318662138	27.19988576143179	22.41614571764034
21	23.65082135415014	26.28518389236188	27.32761082727763	22.736383926210348
22	23.30757676726007	26.011487322692222	27.47596230127249	23.204973608775216
23	23.200213668433133	26.090290779466674	27.831371180147872	22.878124371952318
24	23.438210685537186	26.152963327304075	26.95342662816404	23.4553993589947
25	23.71032060842615	26.17253197093263	27.99082918160759	22.126318239033626
26	23.697362993050486	26.138419065147716	27.18375485249474	22.980463089307058
27	23.70873396164546	26.254773162398585	27.248014047112832	22.78847882884312
28	23.081215159881108	26.09346407302806	27.4727890077111	23.352531759379726
29	23.74126022064968	26.115148245697544	26.758269074138713	23.385322459514065
30	24.037434286379167	25.931890542527427	27.490771004558965	22.53990416653445
31	23.65135023641037	25.84753382202054	27.202530172732946	23.29858576883614
32	22.396577074011784	25.79808333068892	27.853055352817357	23.952284242481937
33	22.736912808470578	25.911793016638633	28.08206137149748	23.269232803393308
34	22.911443954346883	25.887728873798117	27.729561345053366	23.471265826801638
35	23.108188155152902	26.45627730354668	27.456922539904166	22.978612001396247
36	23.614857360454415	26.022593850157076	27.301431155396184	23.06111763399232
37	23.635219327473315	25.68648917378013	27.30857106590931	23.36972043283724
38	23.33613640931256	26.26535080760321	26.873829847999236	23.524682935084993
39	22.98654523529972	25.991125355673322	27.458244745554744	23.56408466347222
40	23.03229355080972	26.39968690170194	27.813653624430128	22.75436592305821
41	23.343805202085914	25.60768571700568	27.54841917092417	23.50008990998424
42	22.84533367181798	26.552798316038885	27.480457800484455	23.12141021165868
43	23.468621415500483	25.78777012661441	27.32311532806567	23.420493129819437
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	19.5
2	32.0
3	114.5
4	197.0
5	197.0
6	257.0
7	317.0
8	315.0
9	313.0
10	419.5
11	526.0
12	526.0
13	834.5
14	1143.0
15	2040.0
16	2937.0
17	3324.5
18	3712.0
19	3712.0
20	3173.5
21	2635.0
22	2718.5
23	2802.0
24	3564.0
25	4326.0
26	4326.0
27	5583.5
28	6841.0
29	8216.0
30	9591.0
31	11180.5
32	12770.0
33	12770.0
34	15090.5
35	17411.0
36	18662.0
37	19913.0
38	21702.5
39	23492.0
40	23492.0
41	25223.5
42	26955.0
43	27919.5
44	28884.0
45	29447.5
46	30011.0
47	30011.0
48	30110.0
49	30209.0
50	30035.0
51	29861.0
52	28869.5
53	27878.0
54	27878.0
55	26115.5
56	24353.0
57	21701.5
58	19050.0
59	17889.5
60	16729.0
61	16729.0
62	14411.0
63	12093.0
64	9856.0
65	7619.0
66	6680.5
67	5742.0
68	5742.0
69	4836.5
70	3931.0
71	3176.0
72	2421.0
73	2078.5
74	1736.0
75	1736.0
76	1231.5
77	727.0
78	553.5
79	380.0
80	306.5
81	233.0
82	233.0
83	198.0
84	163.0
85	141.0
86	119.0
87	86.5
88	54.0
89	54.0
90	46.0
91	38.0
92	20.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	378156.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.18980155947447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.10853395952955	55.675607765988886
2	6.607131962202397	8.350067151376612
3	1.83199288475627	3.4728980053835365
4	0.8755473252460425	2.213026469529043
5	0.49219380085566156	1.5550814302436373
6	0.3550664668727527	1.3461947749267773
7	0.2452559985692509	1.0848374516603203
8	0.18541221465200383	0.9372928840450245
9	0.1479362757310766	0.8413257516204985
>10	1.0033106832258434	12.583321918848181
>50	0.08941459089189192	3.915352638484457
>100	0.05525146890005579	6.095434633083309
>500	0.0021088346908418243	0.8264589566346615
>1k	8.435338763367298E-4	1.103100168175061
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2445	0.6465585631326755	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1694	0.4479632744158495	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	895	0.23667481145347424	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	614	0.1623668538909868	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	539	0.14253376913231577	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	531	0.14041824009139087	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	522	0.13803826992035034	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	430	0.11370968594971388	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	425	0.11238748029913581	No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG	391	0.10339648187520495	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	383	0.10128095283428004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.288822602312274E-4	0.0	0.0
2	0.0	0.0	5.288822602312274E-4	0.0	0.0
3	0.0	0.0	0.0013222056505780683	0.0	0.0
4	0.0	0.0	0.001586646780693682	2.644411301156137E-4	0.0
5	0.0	0.0	0.0021155290409249094	2.644411301156137E-4	0.0
6	0.0	0.0	0.003702175821618591	2.644411301156137E-4	0.0
7	0.0	0.0	0.01004876294439332	2.644411301156137E-4	0.0
8	2.644411301156137E-4	0.0	0.011635409725087002	2.644411301156137E-4	0.0
9	2.644411301156137E-4	0.0	0.017717555717746115	0.0013222056505780683	0.0
10	5.288822602312274E-4	0.0	0.022213054929711547	0.0013222056505780683	0.0
11	5.288822602312274E-4	0.0	0.034906229175261004	0.0013222056505780683	0.0
12	5.288822602312274E-4	0.0	0.03728619934630153	0.0013222056505780683	0.0
13	5.288822602312274E-4	0.0	0.039137287257110825	0.0013222056505780683	0.0
14	5.288822602312274E-4	0.0	0.039401728387226435	0.0013222056505780683	0.0
15	5.288822602312274E-4	0.0	0.04283946307872941	0.0013222056505780683	0.0
16	5.288822602312274E-4	0.0	0.04363278646907626	0.0013222056505780683	0.0
17	5.288822602312274E-4	0.0	0.04548387437988555	0.0013222056505780683	0.0
18	5.288822602312274E-4	0.0	0.047070521160579235	0.0013222056505780683	0.0
19	5.288822602312274E-4	0.0	0.0483927268111573	0.0013222056505780683	0.0
20	5.288822602312274E-4	0.0	0.050243814721966595	0.0013222056505780683	0.0
21	5.288822602312274E-4	0.0	0.05288822602312273	0.001586646780693682	0.0
22	5.288822602312274E-4	0.0	0.05368154941346957	0.0021155290409249094	0.0
23	5.288822602312274E-4	0.0	0.05394599054358519	0.0026444113011561366	0.0
24	5.288822602312274E-4	0.0	0.0542104316737008	0.0029088524312717505	0.0
25	5.288822602312274E-4	0.0	0.05447487280381642	0.003173293561387364	0.0
26	5.288822602312274E-4	0.0	0.05447487280381642	0.0034377346915029777	0.0
27	5.288822602312274E-4	0.0	0.05447487280381642	0.004231058081849819	0.0
28	5.288822602312274E-4	0.0	0.05447487280381642	0.012957615375665069	0.0
29	5.288822602312274E-4	0.0	0.05473931393393203	0.03437734691502978	0.0
30	5.288822602312274E-4	0.0	0.05473931393393203	0.0600281365362443	0.0
31	5.288822602312274E-4	0.0	0.05473931393393203	0.1219073609832979	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACCGT	35	2.3841028E-5	31.714285	6
AGGCCGT	25	0.005491738	29.6	6
GGTATCA	360	0.0	29.291668	1
GTATCAA	1240	0.0	25.512098	1
CGTCCGC	130	0.0	24.192308	29
CCGTCCG	135	0.0	23.296297	28
GTCCGCC	135	0.0	23.296297	30
CCCGTGC	40	0.0019288044	23.125002	8
CGCCGTC	150	0.0	22.2	26
GAGTCGC	135	0.0	21.925926	22
TCCGGCG	145	0.0	21.689655	16
CGAGTCG	140	0.0	21.142857	21
TCCGCCG	150	0.0	20.966667	31
CGCCTGG	150	0.0	20.966667	36
GTCCGGC	170	0.0	20.676472	15
GATCCGT	45	0.0038211325	20.555557	29
AAGTTAG	45	0.0038211325	20.555557	35
AGTTAGA	55	5.1343284E-4	20.181818	36
AGTCGCC	160	0.0	19.65625	23
GCCGTCC	170	0.0	19.588236	27
>>END_MODULE