FastQCFastQC Report
Fri 10 Feb 2017
ERR1632758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723483
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA8140.11251128222777868No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7610.10518560906061372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTAT250.00549501429.61
GGTATCA4400.026.4886361
TATTAGA802.7222995E-823.1250022
TTACATA400.00193049923.1250024
TATCTGT705.0976123E-621.14285736
CTAGACT450.003824459220.5555554
CTTATAC9600.020.23437337
TTAATCC609.232307E-418.53
TTATACC500.00703242418.54
TATTCCG500.00703242418.55
TATACTG500.00703242418.55
GGACACA2500.017.026
GTTTCGA1107.797771E-716.81818215
GTATCAA7400.016.7500021
GTATTAG1452.9722287E-916.5862061
TAGTTCG904.442295E-516.4444457
ATCTAAG1708.54925E-1116.3235332
CATCTAA1708.54925E-1116.3235331
TTATACA4250.016.1058837
ACTATTC1059.333451E-615.8571428
Produced by FastQC (version 0.11.5)