##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632758.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723483
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06254604462026	34.0	33.0	34.0	31.0	34.0
2	33.20237794115411	34.0	33.0	34.0	31.0	34.0
3	33.28211858467994	34.0	34.0	34.0	31.0	34.0
4	36.56313693618233	37.0	37.0	37.0	35.0	37.0
5	36.52956738444442	37.0	37.0	37.0	35.0	37.0
6	36.56857728516081	37.0	37.0	37.0	35.0	37.0
7	36.56069596659493	37.0	37.0	37.0	35.0	37.0
8	36.53628212411349	37.0	37.0	37.0	35.0	37.0
9	38.32367173796758	39.0	39.0	39.0	37.0	39.0
10	38.35846315670168	39.0	39.0	39.0	37.0	39.0
11	38.42655321548675	39.0	39.0	39.0	37.0	39.0
12	38.396230457384625	39.0	39.0	39.0	37.0	39.0
13	38.42285997044851	39.0	39.0	39.0	37.0	39.0
14	40.00038148788568	41.0	40.0	41.0	38.0	41.0
15	39.985771607625885	41.0	40.0	41.0	38.0	41.0
16	39.96241929665244	41.0	40.0	41.0	38.0	41.0
17	39.95828512902169	41.0	40.0	41.0	38.0	41.0
18	39.94083067604906	41.0	40.0	41.0	38.0	41.0
19	39.959180796231564	41.0	40.0	41.0	38.0	41.0
20	39.92393048627265	41.0	40.0	41.0	38.0	41.0
21	39.89441769882637	41.0	40.0	41.0	38.0	41.0
22	39.87356440994467	41.0	40.0	41.0	38.0	41.0
23	39.831819130511704	41.0	40.0	41.0	38.0	41.0
24	39.8117799588933	41.0	40.0	41.0	38.0	41.0
25	39.772728592102375	41.0	40.0	41.0	38.0	41.0
26	39.696704691057015	41.0	40.0	41.0	38.0	41.0
27	39.63195818008163	41.0	40.0	41.0	37.0	41.0
28	39.585933601757056	41.0	40.0	41.0	37.0	41.0
29	39.547227785587225	41.0	40.0	41.0	37.0	41.0
30	39.50327098217927	41.0	40.0	41.0	37.0	41.0
31	39.44141327439622	41.0	39.0	41.0	37.0	41.0
32	39.38854541157152	41.0	39.0	41.0	36.0	41.0
33	39.33465057230094	41.0	39.0	41.0	36.0	41.0
34	39.297560550835335	41.0	39.0	41.0	36.0	41.0
35	39.23041453634709	41.0	39.0	41.0	35.0	41.0
36	39.179156939416686	41.0	39.0	41.0	35.0	41.0
37	39.13565211622112	41.0	39.0	41.0	35.0	41.0
38	39.06376929381893	41.0	39.0	41.0	35.0	41.0
39	38.995597685087276	41.0	39.0	41.0	35.0	41.0
40	38.921211693985896	40.0	39.0	41.0	35.0	41.0
41	38.8747378998539	40.0	39.0	41.0	35.0	41.0
42	38.814766898462025	40.0	38.0	41.0	35.0	41.0
43	38.09733884555684	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	7.0
18	7.0
19	27.0
20	43.0
21	73.0
22	137.0
23	246.0
24	448.0
25	547.0
26	830.0
27	1089.0
28	1539.0
29	2138.0
30	2907.0
31	3899.0
32	5232.0
33	7094.0
34	10592.0
35	16960.0
36	27280.0
37	51463.0
38	133727.0
39	457197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24690421198563	16.250001727753105	12.43595219238047	28.067141867880796
2	20.332751426087412	18.96464740705725	33.05703105670762	27.645570110147716
3	20.707604739848758	19.707719462654964	28.537090712566847	31.04758508492943
4	15.89961339796512	14.926266408471243	32.13054073143391	37.04357946212973
5	17.11733378669575	33.4331283527049	32.8299351885255	16.619602672073842
6	36.829199856803825	32.30483646471306	14.585553496073853	16.28041018240926
7	31.638476646997926	27.527115357237143	19.32471115423583	21.509696841529102
8	27.91523781484845	31.829773470834837	19.257259672998536	20.997729041318177
9	27.657318831264867	13.616491334281525	17.050435186452205	41.67575464800141
10	18.99450298071966	24.443559834854447	29.978175022771786	26.58376216165411
11	39.24708666271356	19.549733718691385	19.066101069410063	22.137078549184984
12	23.48762859666364	23.267305520654944	26.391636016326576	26.853429866354844
13	33.84226028807864	17.54471079486318	21.42800867470279	27.185020242355385
14	24.389239277218678	18.8101171693046	22.539990573379058	34.26065298009767
15	28.583809156538575	24.5761130531056	19.97324055990258	26.86683723045324
16	27.040580082738643	23.557844482869676	21.492004649729157	27.90957078466253
17	27.05260524435267	23.592537765227377	21.754899562256476	27.599957428163478
18	26.601039692708746	22.157673366202108	23.310706678664182	27.930580262424964
19	28.500047685985713	22.405087610904474	22.693276828895772	26.401587874214044
20	28.75810488981773	21.593320091833533	22.88139458701863	26.767180431330107
21	27.278595350547285	22.827212249631298	21.796365636787595	28.097826763033822
22	28.074882201793272	22.55367437797433	21.270852252229837	28.100591168002566
23	27.45233820283269	22.900054320557636	22.188081820858265	27.459525655751417
24	28.291887991839477	22.03576310708061	21.958774428701158	27.713574472378756
25	28.233835487495906	22.02014421900722	22.54220209735405	27.203818196142826
26	27.99181183248259	22.3518728152562	22.308056996501648	27.34825835575957
27	27.30430431675658	21.991117966835432	22.98616553533393	27.718412181074054
28	26.607397824136847	22.66590921970523	22.42595886841847	28.30073408773945
29	27.300710590297218	23.33254547791724	22.137493209930295	27.229250721855248
30	26.762619163131685	23.155761780166223	22.66314481473649	27.4184742419656
31	27.576045325183866	22.315106229171935	22.70848105622385	27.400367389420342
32	25.697079267930278	22.563211575116483	23.093839108866415	28.645870048086824
33	26.355698751732938	21.819586638525024	23.808852454031403	28.015862155710643
34	26.48686976749972	21.666023942511433	23.888881977876466	27.958224312112378
35	26.179606155224107	22.237702890047174	24.109481494382038	27.47320946034668
36	26.029084304676132	21.65925115033802	24.825877042031394	27.485787502954455
37	26.192184197831875	20.754046743323617	24.87674209345624	28.17702696538827
38	25.25892108038475	20.446783131048	26.439736662782675	27.854559125784572
39	24.670932143533435	19.661553899676978	27.460078536745165	28.207435420044426
40	24.23457081921759	20.269031891557923	28.475582702012346	27.02081458721214
41	23.142630856564704	19.987200804994725	29.203450530281984	27.666717808158587
42	22.26534693973459	20.22839513851742	29.684318774594566	27.821939147153422
43	21.250672095958027	20.499859706447836	29.91652879196885	28.33293940562529
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	7.5
2	12.0
3	14.0
4	16.0
5	16.0
6	24.5
7	33.0
8	28.5
9	24.0
10	45.0
11	66.0
12	66.0
13	120.0
14	174.0
15	341.0
16	508.0
17	544.0
18	580.0
19	580.0
20	673.5
21	767.0
22	843.0
23	919.0
24	1143.5
25	1368.0
26	1368.0
27	1774.0
28	2180.0
29	3393.5
30	4607.0
31	5298.0
32	5989.0
33	5989.0
34	7839.0
35	9689.0
36	10892.0
37	12095.0
38	16439.5
39	20784.0
40	20784.0
41	24731.0
42	28678.0
43	31258.5
44	33839.0
45	41492.5
46	49146.0
47	49146.0
48	54967.0
49	60788.0
50	67025.0
51	73262.0
52	77356.5
53	81451.0
54	81451.0
55	74238.5
56	67026.0
57	63424.5
58	59823.0
59	56143.0
60	52463.0
61	52463.0
62	48687.0
63	44911.0
64	39275.0
65	33639.0
66	29630.0
67	25621.0
68	25621.0
69	22169.5
70	18718.0
71	15995.5
72	13273.0
73	10848.0
74	8423.0
75	8423.0
76	6826.5
77	5230.0
78	4283.0
79	3336.0
80	2640.5
81	1945.0
82	1945.0
83	1513.5
84	1082.0
85	821.5
86	561.0
87	411.5
88	262.0
89	262.0
90	191.0
91	120.0
92	85.0
93	50.0
94	32.5
95	15.0
96	15.0
97	10.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	723483.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.1061697970784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.00457607335356	50.55502670441106
2	6.953317727917134	8.080613211027767
3	2.009900874664852	3.503629244957169
4	1.0194738747089709	2.369508882700996
5	0.5913739533313914	1.7181237672921674
6	0.38220514004030726	1.3325086058698932
7	0.287820953000156	1.170692121632879
8	0.22337714330769712	1.0383672174258693
9	0.1697953544267379	0.8879541925567627
>10	1.135718467692772	12.933778938888954
>50	0.12259376618087106	4.99351125116891
>100	0.09769170724518397	10.624637984976468
>500	0.002154964130379119	0.7916478770910488
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	814	0.11251128222777868	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	761	0.10518560906061372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.3822024843707453E-4
10	0.0	0.0	0.0	0.0	1.3822024843707453E-4
11	0.0	0.0	0.0	0.0	1.3822024843707453E-4
12	0.0	0.0	0.0	0.0	1.3822024843707453E-4
13	0.0	0.0	0.0	0.0	1.3822024843707453E-4
14	0.0	0.0	0.0	2.7644049687414906E-4	1.3822024843707453E-4
15	0.0	0.0	0.0	2.7644049687414906E-4	1.3822024843707453E-4
16	0.0	0.0	0.0	5.528809937482981E-4	1.3822024843707453E-4
17	0.0	0.0	0.0	6.911012421853727E-4	1.3822024843707453E-4
18	0.0	1.3822024843707453E-4	0.0	8.293214906224472E-4	1.3822024843707453E-4
19	0.0	1.3822024843707453E-4	0.0	9.675417390595217E-4	1.3822024843707453E-4
20	0.0	1.3822024843707453E-4	0.0	0.0015204227328078199	1.3822024843707453E-4
21	0.0	1.3822024843707453E-4	0.0	0.0029026252171785653	1.3822024843707453E-4
22	0.0	1.3822024843707453E-4	0.0	0.00456126819842346	1.3822024843707453E-4
23	0.0	1.3822024843707453E-4	0.0	0.005943470682794205	2.7644049687414906E-4
24	0.0	1.3822024843707453E-4	0.0	0.008154994657787398	2.7644049687414906E-4
25	0.0	1.3822024843707453E-4	0.0	0.009260756645283995	2.7644049687414906E-4
26	0.0	1.3822024843707453E-4	0.0	0.012163381862462559	2.7644049687414906E-4
27	0.0	1.3822024843707453E-4	0.0	0.03538438359989108	2.7644049687414906E-4
28	0.0	1.3822024843707453E-4	0.0	0.14250507613862384	2.7644049687414906E-4
29	0.0	1.3822024843707453E-4	0.0	0.3083693742631133	2.7644049687414906E-4
30	1.3822024843707453E-4	1.3822024843707453E-4	0.0	0.5144557646827914	2.7644049687414906E-4
31	1.3822024843707453E-4	1.3822024843707453E-4	0.0	1.2070774296009719	2.7644049687414906E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACTAT	25	0.005495014	29.6	1
GGTATCA	440	0.0	26.488636	1
TATTAGA	80	2.7222995E-8	23.125002	2
TTACATA	40	0.001930499	23.125002	4
TATCTGT	70	5.0976123E-6	21.142857	36
CTAGACT	45	0.0038244592	20.555555	4
CTTATAC	960	0.0	20.234373	37
TTAATCC	60	9.232307E-4	18.5	3
TTATACC	50	0.007032424	18.5	4
TATTCCG	50	0.007032424	18.5	5
TATACTG	50	0.007032424	18.5	5
GGACACA	250	0.0	17.02	6
GTTTCGA	110	7.797771E-7	16.818182	15
GTATCAA	740	0.0	16.750002	1
GTATTAG	145	2.9722287E-9	16.586206	1
TAGTTCG	90	4.442295E-5	16.444445	7
ATCTAAG	170	8.54925E-11	16.32353	32
CATCTAA	170	8.54925E-11	16.32353	31
TTATACA	425	0.0	16.10588	37
ACTATTC	105	9.333451E-6	15.857142	8
>>END_MODULE