FastQCFastQC Report
Fri 10 Feb 2017
ERR1632757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570025
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44280.2820337255776182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41960.2672568908138405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32870.2093597235712807No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA26230.1670674033852964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16270.10362892310631995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13850.031.3898931
CGAATTA855.183756E-821.76470615
GTATATA1202.382876E-1020.0416661
CTTATAC7250.019.13793237
CGGGTAA5900.018.18644123
GTATCAA23800.018.1113432
GGTAACG6000.017.88333325
GTAACGT6000.017.88333326
CCGAATT1258.58563E-917.7614
TATACTG1156.409391E-817.695655
TAGACTG852.7241429E-517.4117645
GTATTAG4100.016.6951221
TATTAGA2550.016.6862742
TGCGGGT6450.016.63565821
GCGGTAA4600.016.08695623
TCTTATA12550.016.0677337
TTCTGCG6950.015.97122318
GCGGGTA6900.015.55072522
GTACTAT1101.4524629E-515.1363641
CGTCAAT6850.015.12408730
Produced by FastQC (version 0.11.5)