##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632757.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1570025
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.10400407636821	34.0	33.0	34.0	31.0	34.0
2	33.23163134344995	34.0	33.0	34.0	31.0	34.0
3	33.312244709479145	34.0	34.0	34.0	31.0	34.0
4	36.576693364755336	37.0	37.0	37.0	35.0	37.0
5	36.55502173532268	37.0	37.0	37.0	35.0	37.0
6	36.601029919905734	37.0	37.0	37.0	35.0	37.0
7	36.593518574544994	37.0	37.0	37.0	35.0	37.0
8	36.57141637872009	37.0	37.0	37.0	35.0	37.0
9	38.352878457349405	39.0	39.0	39.0	37.0	39.0
10	38.397779653190234	39.0	39.0	39.0	37.0	39.0
11	38.461203484020956	39.0	39.0	39.0	37.0	39.0
12	38.43988216748141	39.0	39.0	39.0	37.0	39.0
13	38.45671693125906	39.0	39.0	39.0	37.0	39.0
14	40.05162784032101	41.0	40.0	41.0	38.0	41.0
15	40.04063502173532	41.0	40.0	41.0	38.0	41.0
16	40.01892836101336	41.0	40.0	41.0	38.0	41.0
17	40.01977675514721	41.0	40.0	41.0	38.0	41.0
18	40.016021400933106	41.0	40.0	41.0	38.0	41.0
19	40.034896896546236	41.0	40.0	41.0	38.0	41.0
20	39.99190012897884	41.0	40.0	41.0	38.0	41.0
21	39.97400614639894	41.0	40.0	41.0	38.0	41.0
22	39.94188245410105	41.0	40.0	41.0	38.0	41.0
23	39.90492635467588	41.0	40.0	41.0	38.0	41.0
24	39.888076941449974	41.0	40.0	41.0	38.0	41.0
25	39.85050429133294	41.0	40.0	41.0	38.0	41.0
26	39.768064202799316	41.0	40.0	41.0	38.0	41.0
27	39.70169137434117	41.0	40.0	41.0	37.0	41.0
28	39.65749781054442	41.0	40.0	41.0	37.0	41.0
29	39.61598318498113	41.0	40.0	41.0	37.0	41.0
30	39.55927899237273	41.0	40.0	41.0	37.0	41.0
31	39.49070938360854	41.0	40.0	41.0	37.0	41.0
32	39.42600850304932	41.0	40.0	41.0	36.0	41.0
33	39.354016655785735	41.0	39.0	41.0	36.0	41.0
34	39.30011878791739	41.0	39.0	41.0	35.0	41.0
35	39.2238645881435	41.0	39.0	41.0	35.0	41.0
36	39.16049298578048	41.0	39.0	41.0	35.0	41.0
37	39.095254534163466	41.0	39.0	41.0	35.0	41.0
38	39.0092692791516	41.0	39.0	41.0	35.0	41.0
39	38.921668763236255	41.0	39.0	41.0	35.0	41.0
40	38.83547140969093	41.0	39.0	41.0	35.0	41.0
41	38.75573255202943	40.0	39.0	41.0	35.0	41.0
42	38.6660333434181	40.0	38.0	41.0	35.0	41.0
43	37.97356347828856	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	3.0
13	3.0
14	0.0
15	1.0
16	3.0
17	5.0
18	16.0
19	37.0
20	57.0
21	136.0
22	278.0
23	497.0
24	778.0
25	1247.0
26	1828.0
27	2629.0
28	3765.0
29	5315.0
30	6917.0
31	8994.0
32	11420.0
33	15202.0
34	21947.0
35	33968.0
36	54391.0
37	110520.0
38	271811.0
39	1018252.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.05490358433783	17.958822311746626	12.302351873377813	25.683922230537732
2	19.46344803426697	20.707377271062562	34.04837502587538	25.780799668795083
3	20.17904173500422	21.709909077880926	30.012706804031787	28.098342383083068
4	14.775560898711804	15.978025827614209	34.98205442588494	34.26435884778905
5	15.80044903743571	34.856769796659286	33.90589321826086	15.436887947644145
6	33.9136001019092	34.951545357558004	15.584656295281924	15.55019824525087
7	29.501377366602444	29.983407907517396	20.79107020588844	19.72414451999172
8	27.033582267798284	32.672473368258466	20.194264422541043	20.099679941402208
9	26.994793076543367	14.065826977277432	19.130204933042467	39.80917501313673
10	17.942007292877502	25.51131351411602	32.25821244884636	24.288466744160125
11	36.63483065556281	20.99743634655499	21.211700450629767	21.156032547252433
12	22.126781420678014	24.75126192258085	28.814827789366408	24.307128867374722
13	32.09751437079028	19.178484419037915	23.378353847868667	25.345647362303147
14	23.031607776946228	20.617060237894304	24.942596455470454	31.408735529689018
15	26.72581646789064	26.181175458989507	22.29346666454356	24.799541408576296
16	24.814636709606535	25.446664862024488	24.02643270011624	25.71226572825274
17	24.714574608684575	25.538701613031638	24.44037515326189	25.306348625021897
18	24.620372287065493	23.97942707918664	25.831499498415628	25.56870113533224
19	25.930542507284915	24.098788235856116	26.006146398942693	23.964522857916275
20	26.33391188038407	23.58064362032452	25.62812694065381	24.4573175586376
21	25.339341730227225	24.47183962038821	24.94603589114823	25.24278275823633
22	25.5965987802742	24.721389786786833	24.520310186143533	25.16170124679543
23	25.572459037276474	24.275091160968774	25.090237416601646	25.062212385153103
24	25.862518112768907	24.010318307033327	24.702345504052484	25.424818076145282
25	25.72767949554943	23.84528908775338	25.47545421251254	24.951577204184648
26	25.669591248546997	24.2351554911546	25.18214678110221	24.91310647919619
27	25.40220697122657	24.057451314469514	25.476664384325094	25.063677329978823
28	24.833808378847472	24.60776102291365	25.13877167561026	25.419658922628617
29	25.13367621534689	24.99807327908791	24.826738427732042	25.041512077833154
30	25.05743539115619	24.653238005764237	25.33004251524657	24.959284087832998
31	25.531122115889875	24.1889141892645	25.120810178181873	25.159153516663746
32	24.094966640658587	24.37387939682489	25.47691915733826	26.05423480517826
33	24.50572443113963	24.046368688396683	26.105062021305393	25.34284485915829
34	24.70642187226318	24.029298896514387	25.604496743682425	25.659782487540006
35	24.713109663858855	24.759350965748954	25.683731150777856	24.84380821961434
36	24.857247496058978	24.29279788538399	25.86799573255203	24.981958886005
37	25.228833935765355	23.86369643795481	25.578828362605694	25.328641263674147
38	24.557188579799686	24.0697441123549	26.130603015875543	25.242464291969874
39	24.258658301619402	23.770003662362065	26.49002404420312	25.48131399181542
40	24.272479737583797	24.007133644368718	26.839445231763825	24.88094138628366
41	23.623636566296717	23.87936497826468	27.239629942198373	25.257368513240237
42	23.160395535102946	24.198531870511616	27.117721055397208	25.52335153898823
43	23.10714797535071	24.185729526599893	26.891673699463386	25.81544879858601
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	308.0
1	272.0
2	236.0
3	494.5
4	753.0
5	753.0
6	846.5
7	940.0
8	827.0
9	714.0
10	1111.5
11	1509.0
12	1509.0
13	2769.5
14	4030.0
15	6278.0
16	8526.0
17	8524.5
18	8523.0
19	8523.0
20	10154.0
21	11785.0
22	11067.0
23	10349.0
24	11865.0
25	13381.0
26	13381.0
27	15779.0
28	18177.0
29	22544.0
30	26911.0
31	30300.0
32	33689.0
33	33689.0
34	39412.5
35	45136.0
36	49106.0
37	53076.0
38	60650.5
39	68225.0
40	68225.0
41	75786.5
42	83348.0
43	87196.0
44	91044.0
45	101060.0
46	111076.0
47	111076.0
48	116207.5
49	121339.0
50	128849.5
51	136360.0
52	138011.0
53	139662.0
54	139662.0
55	129795.5
56	119929.0
57	112743.0
58	105557.0
59	97507.5
60	89458.0
61	89458.0
62	83053.0
63	76648.0
64	65950.5
65	55253.0
66	48839.5
67	42426.0
68	42426.0
69	36750.5
70	31075.0
71	26610.0
72	22145.0
73	18501.0
74	14857.0
75	14857.0
76	12096.0
77	9335.0
78	7656.0
79	5977.0
80	4829.5
81	3682.0
82	3682.0
83	2923.0
84	2164.0
85	1721.5
86	1279.0
87	987.5
88	696.0
89	696.0
90	498.5
91	301.0
92	201.5
93	102.0
94	65.0
95	28.0
96	28.0
97	20.0
98	12.0
99	8.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1570025.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.86213463459413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.29318134531832	47.646591797250245
2	8.51943947848142	9.51828150316411
3	2.325274692470148	3.896844237995456
4	1.0114702384419165	2.2601154655490947
5	0.5972960320907313	1.6683115680680645
6	0.3771932861142906	1.2642493279308893
7	0.2619133294030421	1.0241726368795279
8	0.19862183947345788	0.8876351950429638
9	0.15284458777388651	0.7684402446354499
>10	1.0259998195130602	11.403274183602853
>50	0.1352141746854445	5.27844189642586
>100	0.0926412868642498	9.867102634096513
>500	0.007196449106640535	2.7235153187730345
>1k	0.0017134402634858415	1.7930239905860572
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4428	0.2820337255776182	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4196	0.2672568908138405	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3287	0.2093597235712807	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2623	0.1670674033852964	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1627	0.10362892310631995	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.369325329214503E-5	0.0
5	0.0	6.369325329214503E-5	0.0	6.369325329214503E-5	0.0
6	0.0	6.369325329214503E-5	0.0	6.369325329214503E-5	0.0
7	0.0	6.369325329214503E-5	0.0	6.369325329214503E-5	0.0
8	6.369325329214503E-5	6.369325329214503E-5	0.0	6.369325329214503E-5	0.0
9	6.369325329214503E-5	6.369325329214503E-5	0.0	6.369325329214503E-5	0.0
10	1.2738650658429006E-4	6.369325329214503E-5	0.0	1.2738650658429006E-4	6.369325329214503E-5
11	1.2738650658429006E-4	6.369325329214503E-5	0.0	2.547730131685801E-4	6.369325329214503E-5
12	1.2738650658429006E-4	6.369325329214503E-5	0.0	3.1846626646072517E-4	6.369325329214503E-5
13	1.2738650658429006E-4	6.369325329214503E-5	0.0	3.1846626646072517E-4	6.369325329214503E-5
14	1.2738650658429006E-4	6.369325329214503E-5	0.0	3.1846626646072517E-4	6.369325329214503E-5
15	1.9107975987643508E-4	6.369325329214503E-5	0.0	4.458527730450152E-4	6.369325329214503E-5
16	1.9107975987643508E-4	6.369325329214503E-5	0.0	5.732392796293053E-4	1.2738650658429006E-4
17	1.9107975987643508E-4	6.369325329214503E-5	0.0	6.369325329214503E-4	1.2738650658429006E-4
18	1.9107975987643508E-4	6.369325329214503E-5	0.0	7.006257862135953E-4	1.9107975987643508E-4
19	1.9107975987643508E-4	6.369325329214503E-5	0.0	7.006257862135953E-4	1.9107975987643508E-4
20	2.547730131685801E-4	6.369325329214503E-5	0.0	9.553987993821755E-4	1.9107975987643508E-4
21	2.547730131685801E-4	6.369325329214503E-5	0.0	0.0013375583191350456	1.9107975987643508E-4
22	2.547730131685801E-4	6.369325329214503E-5	0.0	0.002292957118517221	1.9107975987643508E-4
23	2.547730131685801E-4	6.369325329214503E-5	0.0	0.0030572761580229613	1.9107975987643508E-4
24	2.547730131685801E-4	6.369325329214503E-5	0.0	0.0036942086909444115	1.9107975987643508E-4
25	2.547730131685801E-4	6.369325329214503E-5	0.0	0.004458527730450152	1.9107975987643508E-4
26	2.547730131685801E-4	6.369325329214503E-5	0.0	0.005605006289708762	1.9107975987643508E-4
27	2.547730131685801E-4	6.369325329214503E-5	0.0	0.01165586535246254	1.9107975987643508E-4
28	2.547730131685801E-4	6.369325329214503E-5	0.0	0.041336921386602124	1.9107975987643508E-4
29	2.547730131685801E-4	6.369325329214503E-5	0.0	0.08547634591805862	1.9107975987643508E-4
30	2.547730131685801E-4	6.369325329214503E-5	0.0	0.15776818840464324	1.9107975987643508E-4
31	2.547730131685801E-4	6.369325329214503E-5	0.0	0.4438145889396666	1.9107975987643508E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1385	0.0	31.389893	1
CGAATTA	85	5.183756E-8	21.764706	15
GTATATA	120	2.382876E-10	20.041666	1
CTTATAC	725	0.0	19.137932	37
CGGGTAA	590	0.0	18.186441	23
GTATCAA	2380	0.0	18.111343	2
GGTAACG	600	0.0	17.883333	25
GTAACGT	600	0.0	17.883333	26
CCGAATT	125	8.58563E-9	17.76	14
TATACTG	115	6.409391E-8	17.69565	5
TAGACTG	85	2.7241429E-5	17.411764	5
GTATTAG	410	0.0	16.695122	1
TATTAGA	255	0.0	16.686274	2
TGCGGGT	645	0.0	16.635658	21
GCGGTAA	460	0.0	16.086956	23
TCTTATA	1255	0.0	16.06773	37
TTCTGCG	695	0.0	15.971223	18
GCGGGTA	690	0.0	15.550725	22
GTACTAT	110	1.4524629E-5	15.136364	1
CGTCAAT	685	0.0	15.124087	30
>>END_MODULE