FastQCFastQC Report
Fri 10 Feb 2017
ERR1632756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714242
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29610.4145653714007297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26570.372002766569314No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA25730.360242046813265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21240.29737819954581224No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA14500.20301242436037087No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13750.19251178172104133No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12140.16997040218861395No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11440.1601698023919064No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11200.15680959674732095No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10440.146168945539467No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10150.1421086970522596No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.1418286799152108No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10090.14126864564111324No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9970.13958854281882052No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9910.13874849140767415No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9540.1335681743722716No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9300.13020796872768614No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8970.12558768596638115No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8890.124467617418186No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8870.1241876002811372No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8840.12376757457556403No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8600.12040736893097859No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8600.12040736893097859No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8550.11970732608835662No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA7890.11046676056574661No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT7830.10962670915460027No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA7770.1087866577434539No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC7730.10822662346935633No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7730.10822662346935633No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG7600.1064065120785392No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7590.10626650351001482No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7460.10444639211919769No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7280.10192623788575861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9600.032.9531251
CGTTAGA603.7237445E-521.5833321
TAACTAG450.003824412120.5555535
GTCTTAG1001.2876626E-820.351
GTATCAA15400.020.3019472
GCATGTA656.8974536E-519.9230777
GCGGTAA3750.019.73333223
TCTTAGC1052.2559107E-819.380952
TTCTGCG5300.019.19811418
GTAACGT5250.019.0285726
AACGTCA5500.018.528
GTACCTA701.2183769E-418.51
GCCTTAC500.00703233818.525
GATAACC500.00703233818.56
GACGTGT500.00703233818.534
TAGAACA1002.8711293E-718.54
TGCGGGT5500.018.521
TCTAGAT609.2321495E-418.52
TTATACT500.00703233818.54
ACCTAAC701.2183769E-418.53
Produced by FastQC (version 0.11.5)