##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632756.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	714242
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.10148941115196	34.0	33.0	34.0	31.0	34.0
2	33.22474035410967	34.0	33.0	34.0	31.0	34.0
3	33.303573578703016	34.0	34.0	34.0	31.0	34.0
4	36.556378650373404	37.0	37.0	37.0	35.0	37.0
5	36.54310023773455	37.0	37.0	37.0	35.0	37.0
6	36.597791784857236	37.0	37.0	37.0	35.0	37.0
7	36.597020337644665	37.0	37.0	37.0	35.0	37.0
8	36.57439355288544	37.0	37.0	37.0	35.0	37.0
9	38.35579397459124	39.0	39.0	39.0	37.0	39.0
10	38.400344701095705	39.0	39.0	39.0	37.0	39.0
11	38.47007876882065	39.0	39.0	39.0	37.0	39.0
12	38.443387535317164	39.0	39.0	39.0	37.0	39.0
13	38.46330795444681	39.0	39.0	39.0	37.0	39.0
14	40.04221538358148	41.0	40.0	41.0	38.0	41.0
15	40.0334550474489	41.0	40.0	41.0	38.0	41.0
16	40.00483029561409	41.0	40.0	41.0	38.0	41.0
17	40.01621019206375	41.0	40.0	41.0	38.0	41.0
18	40.007531060900924	41.0	40.0	41.0	38.0	41.0
19	40.018413926932325	41.0	40.0	41.0	38.0	41.0
20	39.981562271611025	41.0	40.0	41.0	38.0	41.0
21	39.95891308548083	41.0	40.0	41.0	38.0	41.0
22	39.917257736173454	41.0	40.0	41.0	38.0	41.0
23	39.880931112984115	41.0	40.0	41.0	38.0	41.0
24	39.85848633936397	41.0	40.0	41.0	38.0	41.0
25	39.81147006196779	41.0	40.0	41.0	38.0	41.0
26	39.7150601616819	41.0	40.0	41.0	37.0	41.0
27	39.6256520899079	41.0	40.0	41.0	37.0	41.0
28	39.58829360356854	41.0	40.0	41.0	37.0	41.0
29	39.54406909702874	41.0	40.0	41.0	37.0	41.0
30	39.47386880077061	41.0	40.0	41.0	36.0	41.0
31	39.39706990067792	41.0	39.0	41.0	36.0	41.0
32	39.31802946340316	41.0	39.0	41.0	35.0	41.0
33	39.232935055625404	41.0	39.0	41.0	35.0	41.0
34	39.167294838444114	41.0	39.0	41.0	35.0	41.0
35	39.06371649944977	41.0	39.0	41.0	35.0	41.0
36	38.98712061178144	41.0	39.0	41.0	35.0	41.0
37	38.90786596139684	41.0	39.0	41.0	35.0	41.0
38	38.80390399892473	41.0	39.0	41.0	35.0	41.0
39	38.68665802347104	40.0	38.0	41.0	35.0	41.0
40	38.5674141257445	40.0	38.0	41.0	35.0	41.0
41	38.472640925624646	40.0	38.0	41.0	35.0	41.0
42	38.3520025425556	40.0	38.0	41.0	35.0	41.0
43	37.629062698637156	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	2.0
14	0.0
15	0.0
16	1.0
17	3.0
18	6.0
19	14.0
20	32.0
21	70.0
22	142.0
23	233.0
24	384.0
25	598.0
26	899.0
27	1301.0
28	1888.0
29	2606.0
30	3492.0
31	4588.0
32	5585.0
33	7245.0
34	10563.0
35	17479.0
36	26355.0
37	55026.0
38	129827.0
39	445899.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	45.47968335662142	16.663959834341863	11.89778814463445	25.95856866440226
2	20.63922312045497	20.5286163513207	32.48226791479639	26.349892613427944
3	21.442592286647944	21.020046426841326	29.338375508581127	28.198985777929607
4	14.861769540295866	16.093004891899383	35.14509087956183	33.90013468824292
5	16.29363717059484	34.18113188527138	33.488789513918256	16.03644143021553
6	33.488229479644154	34.6203387647324	15.777145561308354	16.114286194315092
7	30.109122678307916	29.228468782289475	20.25489959985551	20.4075089395471
8	27.873185838973345	30.936993343992654	20.094729797463604	21.095091019570397
9	28.097339557180902	13.308374472517718	18.31746102861495	40.27682494168643
10	19.555276782939117	24.131736862295973	30.59299229112822	25.71999406363669
11	37.75247045119161	20.421789813536588	20.27982112505285	21.545918610218944
12	22.67550774107375	24.126836562397617	28.180364638315865	25.017291058212766
13	33.84161110659972	18.071325965149068	22.47683558233764	25.61022734591357
14	23.466836170373625	20.108450637178997	24.962967733625298	31.461745458822083
15	28.153342984590658	24.806158136877976	21.948303236158054	25.092195642373312
16	24.584524572903863	24.240803537176475	23.98920813953814	27.185463750381523
17	25.26972650726225	24.559883064843568	23.57814298235052	26.592247445543666
18	25.564864569711666	21.89467995441321	25.338470714407723	27.2019847614674
19	26.976430957574603	22.760912967873633	25.299268315220893	24.96338775933087
20	27.599609096076684	21.89846018576337	24.902624040591284	25.599306677568666
21	26.716155028687755	22.66332699561213	24.498979337535456	26.121538638164655
22	26.95080938953464	23.481817087205737	23.659767977800243	25.907605545459383
23	26.993231985797532	22.630985016283	24.344549886453052	26.031233111466424
24	26.81374100094926	22.293424357570682	24.326908806818977	26.56592583466108
25	26.896486064947172	22.46661495683536	25.228283970979028	25.408615007238446
26	27.06925663850628	23.038549959257505	24.582284435807473	25.309908966428747
27	26.587347145645314	22.565601014782104	25.51488151074846	25.332170328824123
28	25.68163171586101	23.349789007087235	24.425894864765723	26.542684412286032
29	26.281428423419513	23.57436275100036	24.12263630534189	26.02157252023824
30	25.932387062088203	22.949224492538946	24.984249036041007	26.13413940933185
31	26.807160598228613	22.706449634717647	24.165478927310353	26.320910839743394
32	24.873222241201162	23.072292024271885	24.939306285544674	27.11517944898228
33	25.396014236071245	22.820836635202074	25.72783454347406	26.05531458525262
34	25.35275158839721	23.226021432511672	24.88330285813492	26.537924120956202
35	25.659650370602684	24.02211015314137	25.101716225032973	25.216523251222977
36	26.085696444622407	23.381010917868174	24.80251791409634	25.730774723413074
37	26.604708208142334	23.432814088222198	24.384872354188076	25.577605349447385
38	26.015552151791688	23.795716297837426	24.470137572419432	25.71859397795145
39	25.478199265795066	23.09161320672825	25.186421408990228	26.24376611848645
40	25.68065165588134	23.57240263104102	25.460278169023944	25.2866675440537
41	24.62050677501463	23.35986962402099	25.839281364019477	26.180342236944902
42	23.69070987144413	24.08189381190129	25.84894195524766	26.378454361406916
43	23.90296286132711	23.992148319477153	25.535322761753022	26.569566057442717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	109.5
2	92.0
3	219.5
4	347.0
5	347.0
6	422.5
7	498.0
8	441.5
9	385.0
10	550.0
11	715.0
12	715.0
13	1487.5
14	2260.0
15	3436.5
16	4613.0
17	4370.5
18	4128.0
19	4128.0
20	4801.0
21	5474.0
22	4263.0
23	3052.0
24	3358.5
25	3665.0
26	3665.0
27	4304.5
28	4944.0
29	5760.5
30	6577.0
31	7895.0
32	9213.0
33	9213.0
34	11429.0
35	13645.0
36	14799.5
37	15954.0
38	18639.0
39	21324.0
40	21324.0
41	25370.5
42	29417.0
43	33154.0
44	36891.0
45	44209.0
46	51527.0
47	51527.0
48	54648.5
49	57770.0
50	63398.0
51	69026.0
52	70574.5
53	72123.0
54	72123.0
55	67215.5
56	62308.0
57	58695.5
58	55083.0
59	50802.0
60	46521.0
61	46521.0
62	44244.0
63	41967.0
64	34184.5
65	26402.0
66	23213.0
67	20024.0
68	20024.0
69	17332.5
70	14641.0
71	12751.5
72	10862.0
73	9907.5
74	8953.0
75	8953.0
76	8219.0
77	7485.0
78	5340.0
79	3195.0
80	2321.5
81	1448.0
82	1448.0
83	1113.0
84	778.0
85	605.5
86	433.0
87	320.5
88	208.0
89	208.0
90	165.5
91	123.0
92	77.5
93	32.0
94	20.5
95	9.0
96	9.0
97	5.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	714242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.375684306682416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.29896544652432	39.55797893234624
2	7.363902661099887	6.830120501526138
3	2.2349929100365964	3.1094797687059197
4	1.116280555872514	2.070730986273267
5	0.7140730792504812	1.655781384761047
6	0.47976371379393723	1.3349622319625503
7	0.34834477358694127	1.1308309074825527
8	0.27493568666366736	1.0200264487484156
9	0.2148270073691652	0.896647452687157
>10	1.5764569088833515	14.581135822336242
>50	0.21192179083610557	6.84221265524472
>100	0.14673839850584042	13.31169113649506
>500	0.014855746956169641	4.748099429422518
>1k	0.003941320621024599	2.9103023420081935
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2961	0.4145653714007297	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2657	0.372002766569314	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2573	0.360242046813265	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2124	0.29737819954581224	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1450	0.20301242436037087	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1375	0.19251178172104133	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1214	0.16997040218861395	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1144	0.1601698023919064	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1120	0.15680959674732095	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1044	0.146168945539467	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1015	0.1421086970522596	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1013	0.1418286799152108	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1009	0.14126864564111324	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	997	0.13958854281882052	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	991	0.13874849140767415	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	954	0.1335681743722716	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	930	0.13020796872768614	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	897	0.12558768596638115	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	889	0.124467617418186	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	887	0.1241876002811372	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	884	0.12376757457556403	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	860	0.12040736893097859	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	860	0.12040736893097859	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	855	0.11970732608835662	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	789	0.11046676056574661	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	783	0.10962670915460027	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	777	0.1087866577434539	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	773	0.10822662346935633	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	773	0.10822662346935633	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	760	0.1064065120785392	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	759	0.10626650351001482	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	746	0.10444639211919769	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	728	0.10192623788575861	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.400085685243937E-4	0.0
4	0.0	0.0	0.0	1.400085685243937E-4	0.0
5	0.0	0.0	0.0	1.400085685243937E-4	0.0
6	0.0	0.0	0.0	1.400085685243937E-4	0.0
7	0.0	0.0	0.0	1.400085685243937E-4	0.0
8	1.400085685243937E-4	0.0	0.0	1.400085685243937E-4	0.0
9	1.400085685243937E-4	0.0	0.0	2.800171370487874E-4	0.0
10	2.800171370487874E-4	0.0	0.0	4.200257055731811E-4	0.0
11	2.800171370487874E-4	0.0	0.0	7.000428426219685E-4	0.0
12	2.800171370487874E-4	0.0	0.0	8.400514111463622E-4	0.0
13	2.800171370487874E-4	0.0	0.0	8.400514111463622E-4	0.0
14	2.800171370487874E-4	0.0	0.0	0.0012600771167195432	0.0
15	2.800171370487874E-4	0.0	0.0	0.0016801028222927243	0.0
16	2.800171370487874E-4	0.0	0.0	0.001820111390817118	0.0
17	2.800171370487874E-4	0.0	0.0	0.0025201542334390863	0.0
18	2.800171370487874E-4	0.0	0.0	0.00266016280196348	0.0
19	4.200257055731811E-4	0.0	0.0	0.0029401799390122675	0.0
20	4.200257055731811E-4	0.0	0.0	0.003640222781634236	0.0
21	5.600342740975748E-4	0.0	0.0	0.005460334172451354	0.0
22	5.600342740975748E-4	0.0	0.0	0.007000428426219685	0.0
23	5.600342740975748E-4	0.0	0.0	0.0075604627003172595	0.0
24	5.600342740975748E-4	1.400085685243937E-4	0.0	0.00798048840589044	0.0
25	5.600342740975748E-4	1.400085685243937E-4	0.0	0.008820539817036803	0.0
26	5.600342740975748E-4	1.400085685243937E-4	0.0	0.010360634070805133	0.0
27	5.600342740975748E-4	1.400085685243937E-4	0.0	0.01638100251735406	0.0
28	5.600342740975748E-4	1.400085685243937E-4	0.0	0.03640222781634236	0.0
29	7.000428426219685E-4	1.400085685243937E-4	0.0	0.06860419857695291	0.0
30	7.000428426219685E-4	1.400085685243937E-4	0.0	0.12250749745884448	0.0
31	7.000428426219685E-4	1.400085685243937E-4	0.0	0.3148792706113614	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	960	0.0	32.953125	1
CGTTAGA	60	3.7237445E-5	21.583332	1
TAACTAG	45	0.0038244121	20.555553	5
GTCTTAG	100	1.2876626E-8	20.35	1
GTATCAA	1540	0.0	20.301947	2
GCATGTA	65	6.8974536E-5	19.923077	7
GCGGTAA	375	0.0	19.733332	23
TCTTAGC	105	2.2559107E-8	19.38095	2
TTCTGCG	530	0.0	19.198114	18
GTAACGT	525	0.0	19.02857	26
AACGTCA	550	0.0	18.5	28
GTACCTA	70	1.2183769E-4	18.5	1
GCCTTAC	50	0.007032338	18.5	25
GATAACC	50	0.007032338	18.5	6
GACGTGT	50	0.007032338	18.5	34
TAGAACA	100	2.8711293E-7	18.5	4
TGCGGGT	550	0.0	18.5	21
TCTAGAT	60	9.2321495E-4	18.5	2
TTATACT	50	0.007032338	18.5	4
ACCTAAC	70	1.2183769E-4	18.5	3
>>END_MODULE