FastQCFastQC Report
Fri 10 Feb 2017
ERR1632755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775643
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA23660.3050372400704964No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21170.27293484244684735No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17300.22304075457394704No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA16040.20679616782463064No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14520.18719952349212202No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13790.17778797720085143No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13450.17340451728436923No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12850.16566899978469476No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12600.16244586749316373No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12330.15896488461831024No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12110.15612852820176293No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11940.15393679824352183No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC11280.14542772899387993No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11260.14516987841055742No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10710.13807898736918917No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10430.13446907920267442No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10390.13395337803602947No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10380.13382445274436822No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10240.13201949866111085No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10090.13008561928619225No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC10020.12918314224456354No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA9940.12815173991127363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9910.1277649640362899No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9610.12389720528645266No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA9600.12376827999479142No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC9460.12196332591153404No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9420.12144762474488907No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9410.12131869945322783No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9150.11796664187003557No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9120.11757986599505185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.11641953837010068No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC8990.1159038372034557No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT8930.11513028545348826No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC8910.11487243487016578No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8890.11461458428684329No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC8680.11190715316195725No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8560.11036004966202234No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT8560.11036004966202234No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT8380.10803939441212No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC8020.10339808391231532No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC7880.10159312982905797No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7860.10133527924573546No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7850.10120635395407422No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG7790.10043280220410679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.032.7985341
CCTTATA400.001930623923.1252
CAACCGT903.8235157E-922.6111115
GTTATGC502.7010046E-422.21
TAAGTCT1450.021.68965535
ATACGGC1400.021.14285729
CTTATAC11100.021.037
CTAAGTC1500.020.96666734
GTATCAA21350.020.8829042
CTCGTAT806.9541056E-720.812529
TCGTATG806.9541056E-720.812530
TTGGACC450.003824703920.55555537
TAAACCG1101.7516868E-920.1818185
TCTGTCG555.141021E-420.1818188
GCTTAAA1101.7516868E-920.1818182
ACACGAA656.89843E-519.92307717
TATACAC3000.019.73333437
ACCGGGA1052.2566383E-819.380958
AAACCGG1153.0449883E-919.3043486
AACGTCA5500.019.17272628
Produced by FastQC (version 0.11.5)