##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632755.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775643
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70620891312112	34.0	31.0	34.0	31.0	34.0
2	32.852358623748295	34.0	31.0	34.0	31.0	34.0
3	32.93450724108901	34.0	31.0	34.0	31.0	34.0
4	36.35949399401529	37.0	37.0	37.0	35.0	37.0
5	36.26682507287502	37.0	37.0	37.0	35.0	37.0
6	36.29696909531834	37.0	37.0	37.0	35.0	37.0
7	36.30481033155717	37.0	37.0	37.0	35.0	37.0
8	36.275426968334656	37.0	37.0	37.0	35.0	37.0
9	37.99490487247355	39.0	38.0	39.0	35.0	39.0
10	37.98759222993052	39.0	38.0	39.0	35.0	39.0
11	38.06826336342879	39.0	38.0	39.0	37.0	39.0
12	38.0201703618804	39.0	38.0	39.0	35.0	39.0
13	38.065430874770996	39.0	38.0	39.0	37.0	39.0
14	39.468909021289434	40.0	39.0	41.0	37.0	41.0
15	39.45825979219821	40.0	39.0	41.0	37.0	41.0
16	39.40561830636001	40.0	39.0	41.0	37.0	41.0
17	39.40963948620693	40.0	39.0	41.0	37.0	41.0
18	39.43064915173604	40.0	39.0	41.0	37.0	41.0
19	39.42808224917907	40.0	39.0	41.0	37.0	41.0
20	39.41199108352683	40.0	39.0	41.0	37.0	41.0
21	39.36217435082892	40.0	39.0	41.0	37.0	41.0
22	39.32498198269049	40.0	39.0	41.0	37.0	41.0
23	39.28527299285883	40.0	39.0	41.0	36.0	41.0
24	39.26006551983322	40.0	39.0	41.0	36.0	41.0
25	39.20827365166707	40.0	39.0	41.0	36.0	41.0
26	39.109411159515396	40.0	39.0	41.0	36.0	41.0
27	38.99348411575944	40.0	39.0	41.0	35.0	41.0
28	38.972776135412815	40.0	39.0	41.0	35.0	41.0
29	38.921654678763296	40.0	38.0	41.0	35.0	41.0
30	38.86442087403612	40.0	38.0	41.0	35.0	41.0
31	38.797182466676034	40.0	38.0	41.0	35.0	41.0
32	38.74747918823479	40.0	38.0	41.0	35.0	41.0
33	38.6737442354279	40.0	38.0	41.0	35.0	41.0
34	38.63175197868092	40.0	38.0	41.0	35.0	41.0
35	38.532765718249244	40.0	38.0	41.0	35.0	41.0
36	38.46897477318818	40.0	38.0	41.0	35.0	41.0
37	38.40927333837861	40.0	38.0	41.0	35.0	41.0
38	38.34411578522594	40.0	38.0	41.0	34.0	41.0
39	38.24902177934952	40.0	38.0	41.0	34.0	41.0
40	38.15399481462477	40.0	37.0	41.0	34.0	41.0
41	38.08516289065975	40.0	37.0	41.0	34.0	41.0
42	38.003005248548625	40.0	37.0	41.0	34.0	41.0
43	37.22392002506308	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	1.0
13	0.0
14	0.0
15	1.0
16	2.0
17	9.0
18	11.0
19	32.0
20	43.0
21	107.0
22	238.0
23	382.0
24	606.0
25	908.0
26	1280.0
27	1806.0
28	2585.0
29	3379.0
30	4826.0
31	6498.0
32	8468.0
33	11684.0
34	17254.0
35	26939.0
36	45299.0
37	93262.0
38	233435.0
39	316584.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.67524750432867	16.262120588982302	12.735884936755697	27.326746969933335
2	21.615485474632017	19.862746134497442	32.16737597064629	26.354392420224254
3	21.811322992665442	19.609923637549752	29.1746331753139	29.404120194470913
4	15.443831762808404	16.46053661284895	34.89556406748981	33.20006755685283
5	17.314795595396333	32.71453490845659	32.86834278140846	17.10232671473861
6	32.59927569771145	34.3850199125113	15.980032050827505	17.035672338949748
7	29.289247759600745	28.05478809194436	19.772498430334572	22.88346571812032
8	26.860810965869607	30.48309596038384	20.613993809007493	22.04209926473906
9	27.974209784656086	12.7215742293813	17.752883736461232	41.551332249501385
10	19.821876817040827	22.825578262164424	29.232391705978138	28.12015321481661
11	39.18710025101754	19.661751604797566	18.763013396627056	22.388134747557835
12	22.417529714056595	24.04804787769631	26.991025510447457	26.54339689779963
13	36.10269157331401	17.55395201142794	20.476559448096612	25.866796967161438
14	23.97095055328289	20.464182620097134	23.6808686470451	31.88399817957488
15	29.939546930740047	23.739529654750964	20.214067554274326	26.106855860234667
16	24.632337299505057	24.031674365655334	22.984543146782734	28.35144518805688
17	26.43767815863741	23.623625817547506	21.994396906824402	27.944299116990678
18	26.889561305910064	20.869523737080073	23.38820823497408	28.85270672203578
19	28.556178551214927	22.33282579743516	23.129584099901628	25.98141155144828
20	30.38627306634624	20.58653272188365	22.462782491429692	26.564411720340413
21	27.787912738205595	21.862893109329935	21.864955913996518	28.484238238467956
22	28.29742549085082	22.964172950700256	21.487333734720742	27.251067823728185
23	28.573841316172516	21.627862302631495	22.19784101706584	27.60045536413015
24	27.738276500916015	21.094885146903923	23.16168649752528	28.00515185465478
25	28.20769348785459	22.052800063946947	23.082655293736938	26.656851154461524
26	28.155736595315112	22.673446418004158	22.46162216380474	26.709194822875986
27	26.824454033621137	21.998780366740885	24.708145371001866	26.468620228636112
28	26.371152708140215	23.30169936426939	22.017474534031763	28.309673393558633
29	27.05046006990329	23.678547991795195	22.203255879315613	27.067736058985897
30	26.895749719909805	22.393291759224283	23.81598235270608	26.894976168159836
31	28.137944905065858	22.094442933153523	22.58706647259113	27.180545689189483
32	25.20102676102279	22.241933466813986	23.349530647475707	29.20750912468752
33	25.68939576583557	21.831306412872934	24.92538448745106	27.55391333384044
34	25.87285645586952	21.642430860589215	24.549309411675218	27.93540327186605
35	25.637825649171077	23.20139548735694	24.838102064996395	26.322676798475587
36	25.985408235489782	21.913689674244466	25.40898325647237	26.69191883379338
37	26.452246716595134	21.571650875467192	24.796588121081477	27.179514286856193
38	25.22616719289673	20.8721022429133	26.063923738111477	27.83780682607849
39	24.662892593628772	19.751999308960436	27.537024120632818	28.048083976777978
40	24.3667511986829	20.783530567542027	28.446592053302872	26.403126180472203
41	22.80043783029048	20.126785131819663	29.314130340891364	27.75864669699849
42	21.169919666650767	20.848895690414277	30.48683479384201	27.49434984909295
43	20.376384496475826	21.643720113505825	29.545293388839973	28.434602001178376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	11.5
2	16.0
3	29.5
4	43.0
5	43.0
6	71.0
7	99.0
8	105.0
9	111.0
10	157.0
11	203.0
12	203.0
13	429.0
14	655.0
15	1143.0
16	1631.0
17	1571.5
18	1512.0
19	1512.0
20	1770.5
21	2029.0
22	1682.5
23	1336.0
24	1462.0
25	1588.0
26	1588.0
27	1901.5
28	2215.0
29	2839.5
30	3464.0
31	4193.0
32	4922.0
33	4922.0
34	6458.0
35	7994.0
36	8763.0
37	9532.0
38	11957.5
39	14383.0
40	14383.0
41	18274.5
42	22166.0
43	27228.5
44	32291.0
45	46119.0
46	59947.0
47	59947.0
48	67881.5
49	75816.0
50	83701.0
51	91586.0
52	92586.5
53	93587.0
54	93587.0
55	86734.0
56	79881.0
57	75851.5
58	71822.0
59	65986.0
60	60150.0
61	60150.0
62	53990.0
63	47830.0
64	38508.0
65	29186.0
66	25319.5
67	21453.0
68	21453.0
69	18248.0
70	15043.0
71	12437.0
72	9831.0
73	7714.5
74	5598.0
75	5598.0
76	4537.0
77	3476.0
78	2702.5
79	1929.0
80	1509.0
81	1089.0
82	1089.0
83	872.0
84	655.0
85	478.0
86	301.0
87	229.5
88	158.0
89	158.0
90	117.0
91	76.0
92	47.5
93	19.0
94	13.5
95	8.0
96	8.0
97	5.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	775643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.84670025487746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.8440844373668	40.683587925465915
2	6.7904383456660335	6.362192595572852
3	1.8866563099523077	2.651508679089268
4	0.9868253210061825	1.8491804006839943
5	0.6151987502392948	1.441001572481773
6	0.41344069653323584	1.162099943019615
7	0.3084943997273963	1.0116361272036378
8	0.22980070836524155	0.8612323922515985
9	0.1674388127136474	0.7059560283205925
>10	1.3928392619372807	12.933022991069382
>50	0.19455093059014913	6.424123782015749
>100	0.14563681436780768	14.114375694036884
>500	0.018791847015198274	6.219703473258775
>1k	0.005803364519399466	3.580378395529935
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2366	0.3050372400704964	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2117	0.27293484244684735	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1730	0.22304075457394704	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1604	0.20679616782463064	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1452	0.18719952349212202	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1379	0.17778797720085143	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1345	0.17340451728436923	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1285	0.16566899978469476	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1260	0.16244586749316373	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1233	0.15896488461831024	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1211	0.15612852820176293	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1194	0.15393679824352183	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1128	0.14542772899387993	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1126	0.14516987841055742	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1071	0.13807898736918917	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1043	0.13446907920267442	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1039	0.13395337803602947	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1038	0.13382445274436822	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1024	0.13201949866111085	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1009	0.13008561928619225	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	1002	0.12918314224456354	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	994	0.12815173991127363	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	991	0.1277649640362899	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	961	0.12389720528645266	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	960	0.12376827999479142	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	946	0.12196332591153404	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	942	0.12144762474488907	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	941	0.12131869945322783	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	915	0.11796664187003557	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	912	0.11757986599505185	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	903	0.11641953837010068	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	899	0.1159038372034557	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	893	0.11513028545348826	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	891	0.11487243487016578	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	889	0.11461458428684329	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	868	0.11190715316195725	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	856	0.11036004966202234	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	856	0.11036004966202234	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	838	0.10803939441212	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	802	0.10339808391231532	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	788	0.10159312982905797	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	786	0.10133527924573546	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	785	0.10120635395407422	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	779	0.10043280220410679	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.2892529166124106E-4	0.0	0.0	0.0	0.0
9	1.2892529166124106E-4	0.0	0.0	0.0	0.0
10	1.2892529166124106E-4	0.0	0.0	0.0	0.0
11	1.2892529166124106E-4	0.0	0.0	0.0	0.0
12	1.2892529166124106E-4	0.0	0.0	0.0	0.0
13	1.2892529166124106E-4	0.0	0.0	0.0	0.0
14	1.2892529166124106E-4	0.0	0.0	0.0	0.0
15	1.2892529166124106E-4	0.0	0.0	0.0	0.0
16	1.2892529166124106E-4	0.0	0.0	0.0011603276249511697	0.0
17	1.2892529166124106E-4	0.0	0.0	0.0023206552499023393	0.0
18	1.2892529166124106E-4	2.578505833224821E-4	0.0	0.0024495805415635803	0.0
19	2.578505833224821E-4	2.578505833224821E-4	0.0	0.0030942069998697854	0.0
20	2.578505833224821E-4	2.578505833224821E-4	0.0	0.004512385208143437	0.0
21	2.578505833224821E-4	2.578505833224821E-4	0.0	0.006575189874723294	0.0
22	2.578505833224821E-4	2.578505833224821E-4	0.0	0.009282620999609357	0.0
23	2.578505833224821E-4	2.578505833224821E-4	0.0	0.010829724499544249	0.0
24	2.578505833224821E-4	2.578505833224821E-4	0.0	0.013150379749446588	0.0
25	2.578505833224821E-4	2.578505833224821E-4	0.0	0.015342109707687686	0.0
26	2.578505833224821E-4	2.578505833224821E-4	0.0	0.019596644332508642	0.0
27	2.578505833224821E-4	3.867758749837232E-4	0.0	0.047960208497981674	0.0
28	2.578505833224821E-4	3.867758749837232E-4	0.0	0.16373512040977614	0.0
29	2.578505833224821E-4	3.867758749837232E-4	0.0	0.34577763223544855	0.0
30	2.578505833224821E-4	3.867758749837232E-4	0.0	0.6081406007660741	0.0
31	2.578505833224821E-4	3.867758749837232E-4	0.0	1.3840130059834228	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1365	0.0	32.798534	1
CCTTATA	40	0.0019306239	23.125	2
CAACCGT	90	3.8235157E-9	22.61111	15
GTTATGC	50	2.7010046E-4	22.2	1
TAAGTCT	145	0.0	21.689655	35
ATACGGC	140	0.0	21.142857	29
CTTATAC	1110	0.0	21.0	37
CTAAGTC	150	0.0	20.966667	34
GTATCAA	2135	0.0	20.882904	2
CTCGTAT	80	6.9541056E-7	20.8125	29
TCGTATG	80	6.9541056E-7	20.8125	30
TTGGACC	45	0.0038247039	20.555555	37
TAAACCG	110	1.7516868E-9	20.181818	5
TCTGTCG	55	5.141021E-4	20.181818	8
GCTTAAA	110	1.7516868E-9	20.181818	2
ACACGAA	65	6.89843E-5	19.923077	17
TATACAC	300	0.0	19.733334	37
ACCGGGA	105	2.2566383E-8	19.38095	8
AAACCGG	115	3.0449883E-9	19.304348	6
AACGTCA	550	0.0	19.172726	28
>>END_MODULE