FastQCFastQC Report
Fri 10 Feb 2017
ERR1632754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17267
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT350.20269878959865642RNA PCR Primer, Index 17 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA290.16795042566745816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA451.2509403E-424.66666837
CTCTTAT650.001496339717.07692336
TCTCTTA850.00892294613.05882335
CCTGTCT1350.001259789310.96296336
TGTCTCT1706.1068844E-510.88235337
CTGTCTC2259.005345E-69.86666737
Produced by FastQC (version 0.11.5)