##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632754.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17267
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.020038223200324	34.0	33.0	34.0	31.0	34.0
2	33.16615509353101	34.0	33.0	34.0	31.0	34.0
3	33.25893322522731	34.0	34.0	34.0	31.0	34.0
4	36.555568425319976	37.0	37.0	37.0	35.0	37.0
5	36.512017142526204	37.0	37.0	37.0	35.0	37.0
6	36.54427520704233	37.0	37.0	37.0	35.0	37.0
7	36.550703654369606	37.0	37.0	37.0	35.0	37.0
8	36.503851277002376	37.0	37.0	37.0	35.0	37.0
9	38.291133375803554	39.0	39.0	39.0	37.0	39.0
10	38.35518619331673	39.0	39.0	39.0	37.0	39.0
11	38.41165228470493	39.0	39.0	39.0	37.0	39.0
12	38.37001216192738	39.0	39.0	39.0	37.0	39.0
13	38.405629234956855	39.0	39.0	39.0	37.0	39.0
14	39.94642960560607	41.0	40.0	41.0	38.0	41.0
15	39.946140035906645	41.0	40.0	41.0	38.0	41.0
16	39.93502055944866	41.0	40.0	41.0	38.0	41.0
17	39.93808999826258	41.0	40.0	41.0	38.0	41.0
18	39.92888168182081	41.0	40.0	41.0	38.0	41.0
19	39.928592112121386	41.0	40.0	41.0	38.0	41.0
20	39.84953958417791	41.0	40.0	41.0	38.0	41.0
21	39.85168239995367	41.0	40.0	41.0	38.0	41.0
22	39.8531302484508	41.0	40.0	41.0	38.0	41.0
23	39.796200845543524	41.0	40.0	41.0	38.0	41.0
24	39.78102739329356	41.0	40.0	41.0	38.0	41.0
25	39.7501013493948	41.0	40.0	41.0	38.0	41.0
26	39.65402212312504	41.0	40.0	41.0	37.0	41.0
27	39.60815428273585	41.0	40.0	41.0	37.0	41.0
28	39.55394683500319	41.0	40.0	41.0	37.0	41.0
29	39.534429837261825	41.0	40.0	41.0	37.0	41.0
30	39.494642960560604	41.0	40.0	41.0	37.0	41.0
31	39.42155556842532	41.0	39.0	41.0	36.0	41.0
32	39.36618984189494	41.0	39.0	41.0	36.0	41.0
33	39.281114264203396	41.0	39.0	41.0	36.0	41.0
34	39.244280998436324	41.0	39.0	41.0	36.0	41.0
35	39.2029304453582	41.0	39.0	41.0	35.0	41.0
36	39.10980483002259	41.0	39.0	41.0	35.0	41.0
37	39.068628018764116	41.0	39.0	41.0	35.0	41.0
38	38.97654485434644	41.0	39.0	41.0	35.0	41.0
39	38.88793652632189	40.0	39.0	41.0	35.0	41.0
40	38.84021543985637	40.0	38.0	41.0	35.0	41.0
41	38.78027451207505	40.0	38.0	41.0	35.0	41.0
42	38.71008281693403	40.0	38.0	41.0	35.0	41.0
43	37.999305032721374	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	1.0
23	8.0
24	10.0
25	16.0
26	22.0
27	31.0
28	40.0
29	58.0
30	87.0
31	103.0
32	116.0
33	163.0
34	290.0
35	374.0
36	683.0
37	1211.0
38	3364.0
39	10689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.163259396536745	16.430184745468235	12.283546649678577	28.12300920831644
2	20.970637632478137	19.18688828400996	31.655759541321597	28.186714542190305
3	21.213876179996525	19.50541495337928	27.578618173394336	31.702090693229863
4	16.505472867319163	15.156078067990967	31.37777262987201	36.96067643481786
5	17.739039786876702	32.22331615219783	32.692419065269014	17.345224995656455
6	37.256037528233044	32.26385591011757	14.397405455493137	16.082701106156254
7	31.678925117275732	27.26009150402502	19.01314646435397	22.047836914345282
8	27.70023744715353	31.302484508021085	19.459083801471014	21.538194243354376
9	27.83343950888979	13.094341808073203	16.528638443273298	42.54358023976371
10	18.99577228238837	23.81421208084786	29.622980251346497	27.56703538541727
11	39.775293913244916	19.007355070365435	18.69461979498465	22.522731221404992
12	24.3643945097585	22.621184919210055	25.731163491052296	27.28325707997915
13	34.875774598945966	16.887704870562345	20.93588926854694	27.30063126194475
14	24.74662651300168	18.10389760815428	22.29107546186367	34.858400416980366
15	28.78322812300921	23.918457172641457	19.41275264956275	27.88556205478659
16	27.39329356576128	23.136618984189493	21.034342966352	28.435744483697228
17	27.56703538541727	22.48219146348526	21.265998725893322	28.684774425204147
18	26.83731974286211	21.839346730758095	22.89917183065964	28.424161695720162
19	28.580529333410553	22.360572189726067	22.198413158047142	26.86048531881624
20	29.825679040945158	20.70423350900562	22.389529159668733	27.080558290380495
21	28.059303874442577	22.030462732379682	21.53240284936584	28.377830543811893
22	29.130711762321194	22.44744309955406	20.489951931429896	27.931893206694852
23	28.870099032837203	21.630856547170904	21.781432790872763	27.717611629119126
24	28.51682399953669	21.46869751549198	21.445531939537847	28.568946545433487
25	28.771645335032144	21.972548792494354	21.978340186482885	27.27746568599062
26	27.87977066079805	22.320032431806336	21.33549545375572	28.46470145363989
27	28.2562112700527	21.387617999652516	22.3142410378178	28.04192969247698
28	26.848902530839176	22.343198007760467	21.515028667400244	29.292870794000116
29	26.80836277291944	23.345109167776684	22.169456188104476	27.6770718711994
30	26.912607864713035	23.0497480743615	22.55747958533619	27.480164475589277
31	27.740777205073265	22.343198007760467	21.596108183239707	28.319916603926565
32	26.009150402501884	21.648230729136504	22.644350495164186	29.69826837319743
33	26.18289222215787	21.769850002895698	23.993745294492385	28.053512480454046
34	26.69253489314878	21.596108183239707	23.733132565008397	27.978224358603114
35	26.061272948398678	21.955174610528754	24.393351479701163	27.5902009613714
36	26.07864713036428	21.20808478600799	24.09799038628598	28.61527769734175
37	25.864365552788556	20.339375687728037	25.84119997683442	27.955058782648983
38	25.16939827416459	20.223547807957377	26.142352464238144	28.46470145363989
39	24.375977297735567	19.320090345746223	27.90872763074072	28.395204725777496
40	23.895291596687322	19.696530955000867	29.136503156309722	27.271674292002086
41	22.412694735622864	19.430126831528348	29.72143394915156	28.435744483697228
42	21.619273759193838	19.7544448948862	30.225285226153936	28.400996119766024
43	21.20808478600799	20.316210111773906	30.775467655064574	27.70023744715353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.0
7	3.0
8	2.0
9	1.0
10	3.5
11	6.0
12	6.0
13	5.5
14	5.0
15	12.5
16	20.0
17	18.0
18	16.0
19	16.0
20	19.0
21	22.0
22	21.0
23	20.0
24	22.0
25	24.0
26	24.0
27	37.5
28	51.0
29	62.0
30	73.0
31	92.0
32	111.0
33	111.0
34	144.5
35	178.0
36	244.0
37	310.0
38	374.0
39	438.0
40	438.0
41	508.5
42	579.0
43	672.5
44	766.0
45	914.5
46	1063.0
47	1063.0
48	1211.0
49	1359.0
50	1463.5
51	1568.0
52	1732.0
53	1896.0
54	1896.0
55	1807.0
56	1718.0
57	1646.5
58	1575.0
59	1480.5
60	1386.0
61	1386.0
62	1284.0
63	1182.0
64	1029.0
65	876.0
66	780.0
67	684.0
68	684.0
69	573.5
70	463.0
71	388.0
72	313.0
73	273.0
74	233.0
75	233.0
76	193.5
77	154.0
78	111.5
79	69.0
80	63.5
81	58.0
82	58.0
83	39.5
84	21.0
85	15.5
86	10.0
87	8.5
88	7.0
89	7.0
90	6.0
91	5.0
92	4.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	17267.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.57745989459663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.28792487766168	81.69919499623559
2	4.000793545827271	7.007586726124979
3	1.2233831503769343	3.2142236636358374
4	0.6149980161354318	2.154398563734291
5	0.2512895119693162	1.1003648578212777
6	0.22483798439359876	1.18144437366074
7	0.09258034651501125	0.5675566108762379
8	0.0793545827271525	0.5559738228991719
9	0.03967729136357625	0.31273527538078416
>10	0.1851606930300225	2.206521109631088
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	35	0.20269878959865642	RNA PCR Primer, Index 17 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	29	0.16795042566745816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.00579139398853304	0.0
23	0.0	0.0	0.0	0.00579139398853304	0.0
24	0.0	0.0	0.0	0.00579139398853304	0.0
25	0.0	0.0	0.0	0.00579139398853304	0.0
26	0.0	0.0	0.0	0.00579139398853304	0.0
27	0.0	0.0	0.0	0.04633115190826432	0.0
28	0.0	0.0	0.0	0.20849018358718943	0.0
29	0.0	0.0	0.0	0.42277176116291193	0.0
30	0.0	0.0	0.0	0.7007586726124978	0.0
31	0.0	0.0	0.0	1.44784849713326	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTATA	45	1.2509403E-4	24.666668	37
CTCTTAT	65	0.0014963397	17.076923	36
TCTCTTA	85	0.008922946	13.058823	35
CCTGTCT	135	0.0012597893	10.962963	36
TGTCTCT	170	6.1068844E-5	10.882353	37
CTGTCTC	225	9.005345E-6	9.866667	37
>>END_MODULE