FastQCFastQC Report
Fri 10 Feb 2017
ERR1632753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111376
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA1210.10864099985634249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGT200.001834807337.04
AGAGGTC200.001834807337.08
GGTATCA1350.032.8888851
ACCCGAT250.005475314329.632
GTATCAA1750.025.371432
GGCTAAC701.8906758E-723.7857131
TAATACG551.8827317E-523.54545427
ACTGCTC400.00192031423.1258
GGTAATA652.6469006E-622.7692325
GCGGTAA652.6469006E-622.7692323
CGTGCCA652.6469006E-622.7692310
GTAATAC652.6469006E-622.7692326
CTAACTC652.6469006E-622.769233
GCTAACT652.6469006E-622.769232
TCCGTGC753.6783968E-722.28
AACTCCG753.6783968E-722.25
ATACGGA603.687534E-521.58333429
AATACGG603.687534E-521.58333428
CGCGGTA705.0339913E-621.14285722
CCGCGGT705.0339913E-621.14285721
Produced by FastQC (version 0.11.5)