FastQCFastQC Report
Fri 10 Feb 2017
ERR1632752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32341
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT2470.7637364336291395TruSeq Adapter, Index 11 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1810.5596611112828916No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1260.3895983426610185No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1160.3586778392752234No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1070.3308493862280078No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1050.3246652855508488No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1040.3215732352122692No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1030.3184811848736897No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1000.3092050338579512No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA980.30302093318079215No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT950.2937447821650536No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA880.27210042979499705No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT880.27210042979499705No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC820.25354812776352No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC790.24427197674778148No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC780.24117992640920197No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA780.24117992640920197No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA770.23808787607062246No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC760.23499582573204295No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA760.23499582573204295No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT750.23190377539346343No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT730.22571967471630436No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC710.21953557403914534No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC680.2102594230234068No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA670.2071673726848273No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG670.2071673726848273No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA650.20098327200766827No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC640.19789122166908876No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT630.19479917133050925No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC620.19170712099192974No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG580.1793389196376117No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA570.1762468692990322No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT570.1762468692990322No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA560.17315481896045268No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC550.17006276862187317No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT540.16697071828329366No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC540.16697071828329366No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC540.16697071828329366No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC530.16387866794471415No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT520.1607866176061346No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG510.1576945672675551No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC500.1546025169289756RNA PCR Primer, Index 11 (95% over 22bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG490.15151046659039608No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC480.14841841625181657No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA480.14841841625181657No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC470.14532636591323705No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC470.14532636591323705No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA470.14532636591323705No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC460.14223431557465754No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC440.13605021489749852No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC440.13605021489749852No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA440.13605021489749852No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC430.132958164558919No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC420.1298661142203395No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG420.1298661142203395No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC420.1298661142203395No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT410.12677406388176No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT410.12677406388176No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC410.12677406388176No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC410.12677406388176No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT400.1236820135431805No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG400.1236820135431805No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG400.1236820135431805No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT400.1236820135431805No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA390.12058996320460098No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA390.12058996320460098No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT390.12058996320460098No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT380.11749791286602147No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA380.11749791286602147No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG380.11749791286602147No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA380.11749791286602147No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA380.11749791286602147No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT370.11440586252744193No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTG370.11440586252744193No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT370.11440586252744193No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC370.11440586252744193No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC370.11440586252744193No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA360.11131381218886242No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG360.11131381218886242No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA360.11131381218886242No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG350.10822176185028291No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG350.10822176185028291No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG350.10822176185028291No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACCTGTCTCTTAT340.1051297115117034No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC340.1051297115117034No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG340.1051297115117034No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT340.1051297115117034No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA330.10203766117312389No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC330.10203766117312389No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG330.10203766117312389No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGG330.10203766117312389No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA330.10203766117312389No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCA352.3177574E-531.71428528
GGTATCA751.2732926E-1129.61
TTCATAT250.005418597329.5999982
AATCTGC250.005418597329.5999986
CGATAAG250.005418597329.59999812
GCGATAA250.005418597329.59999811
GATAAGC250.005418597329.59999813
CCGTCTT502.585075E-729.59999837
GTTCATA250.005418597329.5999981
TCTGCGA250.005418597329.5999988
CTGCGAT250.005418597329.5999989
TAATCTG250.005418597329.5999985
CTAATCT250.005418597329.5999984
GCCGTCT502.585075E-729.59999836
ATCTGCG250.005418597329.5999987
CGGGTAA453.8521175E-628.77777723
TCTGCGG453.8521175E-628.77777719
TTCTTCT453.8521175E-628.77777715
TCTTCTG453.8521175E-628.77777716
GTAACGT453.8521175E-628.77777726
Produced by FastQC (version 0.11.5)