FastQCFastQC Report
Fri 10 Feb 2017
ERR1632750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174552
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2160.1237453595490169No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1870.1071313992391952No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1750.1002566570420276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAT200.001837662837.01
TACACTG200.001837662837.05
TACGGCT251.2293409E-436.99999630
TAAACTA250.0054837429.5999985
ACTACCC250.0054837429.59999827
TGTACAC250.0054837429.5999983
GGTATCA1400.023.7857131
CATCAGA701.9035542E-723.78571316
TAAAGAC400.001924667623.12500237
CCTCGTT400.001924667623.12500223
CCTAAAC400.001924667623.1250023
ATCAGAA753.7032987E-722.217
GTGTGTA502.6894012E-422.1999999
TAATACG603.703032E-521.58333427
CTTATAC1750.021.14285737
TAGAAGA450.003813016520.55555514
GGTTTCT450.003813016520.55555515
TGCATTA450.003813016520.55555519
TCAGAAG902.1310207E-618.518
GCTCAGA609.194101E-418.51
Produced by FastQC (version 0.11.5)