FastQCFastQC Report
Fri 10 Feb 2017
ERR1632749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17905
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA240.13404077073443174No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA220.12287070650656243No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT210.11728567439262777RNA PCR Primer, Index 29 (95% over 23bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA210.11728567439262777No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA190.10611561016475844No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA190.10611561016475844No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA190.10611561016475844No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA180.10053057805082379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT500.00677437918.537
CTGTCTC1450.00229366110.20689737
Produced by FastQC (version 0.11.5)