##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632749.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17905
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.016531695057246	34.0	33.0	34.0	31.0	34.0
2	33.16542865121475	34.0	33.0	34.0	31.0	34.0
3	33.24188774085451	34.0	34.0	34.0	31.0	34.0
4	36.5396816531695	37.0	37.0	37.0	35.0	37.0
5	36.49488969561575	37.0	37.0	37.0	35.0	37.0
6	36.539234850600394	37.0	37.0	37.0	35.0	37.0
7	36.521977101368336	37.0	37.0	37.0	35.0	37.0
8	36.502094387042725	37.0	37.0	37.0	35.0	37.0
9	38.28483663781067	39.0	39.0	39.0	37.0	39.0
10	38.31036023457135	39.0	39.0	39.0	37.0	39.0
11	38.37252164199944	39.0	39.0	39.0	37.0	39.0
12	38.32834403797822	39.0	39.0	39.0	37.0	39.0
13	38.36330633901145	39.0	39.0	39.0	37.0	39.0
14	39.92147444847808	41.0	40.0	41.0	38.0	41.0
15	39.89533649818487	41.0	40.0	41.0	38.0	41.0
16	39.89427534208322	41.0	40.0	41.0	38.0	41.0
17	39.87997765987154	41.0	40.0	41.0	38.0	41.0
18	39.87947500698129	41.0	40.0	41.0	38.0	41.0
19	39.889583915107515	41.0	40.0	41.0	38.0	41.0
20	39.83004747277297	41.0	40.0	41.0	38.0	41.0
21	39.84389835241553	41.0	40.0	41.0	38.0	41.0
22	39.779111979893884	41.0	40.0	41.0	38.0	41.0
23	39.7449315833566	41.0	40.0	41.0	38.0	41.0
24	39.71415805640883	41.0	40.0	41.0	38.0	41.0
25	39.675397933538115	41.0	40.0	41.0	37.0	41.0
26	39.58223959787769	41.0	40.0	41.0	37.0	41.0
27	39.521195196872384	41.0	40.0	41.0	37.0	41.0
28	39.47137671041609	41.0	40.0	41.0	37.0	41.0
29	39.410444010053055	41.0	40.0	41.0	37.0	41.0
30	39.35721865400726	41.0	39.0	41.0	36.0	41.0
31	39.3077352694778	41.0	39.0	41.0	36.0	41.0
32	39.234124546216144	41.0	39.0	41.0	36.0	41.0
33	39.175705110304385	41.0	39.0	41.0	35.0	41.0
34	39.127506283161125	41.0	39.0	41.0	35.0	41.0
35	39.038871823512984	41.0	39.0	41.0	35.0	41.0
36	38.97257749232058	41.0	39.0	41.0	35.0	41.0
37	38.91538676347389	40.0	39.0	41.0	35.0	41.0
38	38.863725216419994	40.0	39.0	41.0	35.0	41.0
39	38.77335939681653	40.0	38.0	41.0	35.0	41.0
40	38.69176207763195	40.0	38.0	41.0	35.0	41.0
41	38.64177604021223	40.0	38.0	41.0	35.0	41.0
42	38.563585590617144	40.0	38.0	41.0	35.0	41.0
43	37.828371963138785	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	2.0
20	0.0
21	3.0
22	3.0
23	9.0
24	15.0
25	15.0
26	25.0
27	39.0
28	56.0
29	58.0
30	97.0
31	110.0
32	139.0
33	211.0
34	279.0
35	442.0
36	696.0
37	1429.0
38	3462.0
39	10813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36218933258866	15.850321139346551	12.058084333984919	28.729405194079867
2	21.290142418318904	18.603741971516335	30.723261658754534	29.38285395141022
3	20.61435353253281	19.39123149958112	27.735269477799495	32.25914549008657
4	16.18542306618263	14.694219491762079	31.365540351857025	37.754817090198266
5	17.581681094666294	32.15302987992181	32.90700921530299	17.358279810108908
6	37.29125942474169	32.28707065065624	14.163641440938285	16.258028483663782
7	32.43228148561854	27.55654845015359	19.123149958112258	20.888020106115608
8	29.14269757051103	30.578050823792235	18.514381457693382	21.76487014800335
9	27.651493996090476	12.504886903099694	16.950572465791677	42.89304663501815
10	19.39681653169506	23.211393465512426	29.354928790840546	28.03686121195197
11	40.68695895001396	18.5981569394024	17.69896676905892	23.015917341524712
12	24.04356325048869	22.747835800055853	25.77492320580843	27.433677743647028
13	35.822395978776875	16.49818486456297	19.905054454063112	27.774364702597037
14	25.222005026528905	18.240714884110584	21.686679698408266	34.850600390952245
15	29.086847249371683	23.89835241552639	19.25719072884669	27.757609606255233
16	27.712929349343757	22.826026249650937	20.94945545936889	28.51158894163641
17	28.187657079028206	22.49650935492879	20.759564367495113	28.55626919854789
18	27.266126780228987	21.373917900027926	21.99385646467467	29.36609885506842
19	29.500139625802845	21.52471376710416	21.999441496788606	26.975705110304382
20	29.555989946942198	20.642278693102487	21.675509634180397	28.126221725774926
21	28.15973191845853	21.345992739458254	20.592013404077072	29.90226193800614
22	29.220888020106116	22.31778832728288	20.318346830494274	28.14297682211673
23	29.204132923764313	22.278693102485338	21.027645908963976	27.489528064786374
24	28.80201061156102	20.966210555710695	20.586428371963137	29.64535046076515
25	29.354928790840546	21.049986037419714	22.01061156101648	27.584473610723258
26	28.081541468863445	22.12231220329517	21.45210834962301	28.344037978218374
27	27.886065344875732	21.396258028483665	21.625244345154986	29.09243228148562
28	26.796984082658476	22.09997207483943	22.094387042725497	29.0086567997766
29	28.42222842781346	22.26752303825747	21.52471376710416	27.78553476682491
30	27.467187936330635	21.792795308573023	22.289863166713207	28.45015358838313
31	28.237922368053614	21.71460485897794	21.64199944149679	28.405473331471654
32	26.506562412733874	20.932700363027088	22.92655682770176	29.63418039653728
33	26.796984082658476	21.709019826864004	23.233733593968164	28.260262496509352
34	27.037140463557662	21.083496230103325	22.93772689192963	28.941636414409384
35	26.796984082658476	22.05529181792795	23.64702597039933	27.50069812901424
36	27.383412454621613	21.368332867913992	23.149958112259146	28.09829656520525
37	27.02038536721586	21.20078190449595	23.328679139905052	28.45015358838313
38	25.763753141580565	20.61993856464675	25.121474448478075	28.494833845294607
39	25.70790282044122	20.223401284557387	25.30019547612399	28.76850041887741
40	25.015358838313322	21.11700642278693	26.39486177045518	27.47277296844457
41	24.523876012287072	19.871544261379505	27.132086009494554	28.47249371683887
42	23.116447919575535	20.117285674392626	28.433398492041327	28.332867913990505
43	23.021502373638647	19.94973471097459	27.936330633901147	29.09243228148562
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	1.5
7	2.0
8	2.5
9	3.0
10	2.0
11	1.0
12	1.0
13	3.0
14	5.0
15	11.5
16	18.0
17	16.0
18	14.0
19	14.0
20	17.0
21	20.0
22	16.5
23	13.0
24	16.5
25	20.0
26	20.0
27	23.5
28	27.0
29	51.0
30	75.0
31	91.0
32	107.0
33	107.0
34	138.0
35	169.0
36	203.5
37	238.0
38	311.0
39	384.0
40	384.0
41	467.0
42	550.0
43	642.0
44	734.0
45	856.5
46	979.0
47	979.0
48	1146.5
49	1314.0
50	1464.0
51	1614.0
52	1700.0
53	1786.0
54	1786.0
55	1816.5
56	1847.0
57	1744.0
58	1641.0
59	1617.5
60	1594.0
61	1594.0
62	1480.0
63	1366.0
64	1200.5
65	1035.0
66	915.0
67	795.0
68	795.0
69	672.5
70	550.0
71	464.5
72	379.0
73	305.0
74	231.0
75	231.0
76	189.0
77	147.0
78	129.0
79	111.0
80	88.5
81	66.0
82	66.0
83	49.5
84	33.0
85	25.5
86	18.0
87	13.0
88	8.0
89	8.0
90	7.5
91	7.0
92	4.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	17905.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.08182072046914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.1246793227296	81.09466629433119
2	4.117496151872755	7.171181234292097
3	1.2698819907644945	3.3175090756771852
4	0.525910723447922	1.8318905333705666
5	0.2821959979476654	1.2287070650656242
6	0.2052334530528476	1.072326165875454
7	0.14751154438173422	0.8991901703434795
8	0.08978963571062083	0.6255235967606814
9	0.044894817855310416	0.35185702317788325
>10	0.19240636223704463	2.4071488411058364
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	24	0.13404077073443174	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	22	0.12287070650656243	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	21	0.11728567439262777	RNA PCR Primer, Index 29 (95% over 23bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	21	0.11728567439262777	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	19	0.10611561016475844	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	19	0.10611561016475844	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	19	0.10611561016475844	No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA	18	0.10053057805082379	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.005585032113934655	0.0
21	0.0	0.0	0.0	0.005585032113934655	0.0
22	0.0	0.0	0.0	0.005585032113934655	0.0
23	0.0	0.0	0.0	0.005585032113934655	0.0
24	0.0	0.0	0.0	0.005585032113934655	0.0
25	0.0	0.0	0.0	0.005585032113934655	0.0
26	0.0	0.0	0.0	0.005585032113934655	0.0
27	0.0	0.0	0.0	0.039095224797542585	0.0
28	0.0	0.0	0.0	0.1340407707344317	0.0
29	0.0	0.0	0.0	0.3295168947221446	0.0
30	0.0	0.0	0.0	0.4970678581401843	0.0
31	0.0	0.0	0.0	0.9606255235967607	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTTAT	50	0.006774379	18.5	37
CTGTCTC	145	0.002293661	10.206897	37
>>END_MODULE