FastQCFastQC Report
Fri 10 Feb 2017
ERR1632747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128164
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT2990.23329484098498798TruSeq Adapter, Index 6 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2310.1802378202927499No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1830.14278580568646423No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1790.13966480446927373No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1690.13186230142629757No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1680.13108205112199994No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1660.1295215505134047No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1640.12796104990480944No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1430.11157579351455946No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1420.11079554321026186No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1400.10923504260166661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1100.033.6363641
TACGGCT303.5818145E-430.83333430
CTGGTCG250.005478364429.59999821
CAAGCGA250.005478364429.5999989
ATACGGC250.005478364429.59999829
CTAACTC851.8189894E-1228.2941173
TAACTCC851.8189894E-1228.2941174
TAATACG803.092282E-1127.75000227
GTAATAC905.456968E-1226.72222126
GCTAACT905.456968E-1226.7222212
GGCCGGT358.8262814E-426.42857228
TGACCAC358.8262814E-426.42857210
CGGAGCT358.8262814E-426.42857232
AACTCCG959.094947E-1225.315795
CGGTAAT959.094947E-1225.3157924
CTCCGTT400.001921888923.12500212
ATTGTAG400.001921888923.1250021
TTCCGAC400.001921888923.12500218
GTTTTGA400.001921888923.1250021
TTTTGAC400.001921888923.1250022
Produced by FastQC (version 0.11.5)