FastQCFastQC Report
Fri 10 Feb 2017
ERR1632746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64569
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2880.4460344747479441No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT2400.3716953956232867Illumina PCR Primer Index 10 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2330.36085427991760755No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2180.3376233176911521No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2110.3267822019854729No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1910.2958075856835323No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1890.2927101240533383No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1850.2865152007929502No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1830.28341773916275614No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1740.26947916182688286No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1620.2508943920457185No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1530.23695581470984528No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1440.22301723737397205No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1400.2168223141135839No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1400.2168223141135839No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1390.2152735832984869No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1380.21372485248338985No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1300.2013350059626136No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1280.19823754433241958No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1260.19514008270222552No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1260.19514008270222552No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1180.1827502361814493No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA1150.17810404373615824No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1130.17500658210596415No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1120.17345785129086713No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1090.16881165884557606No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1090.16881165884557606No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1040.16106800477009092No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT1040.16106800477009092No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1030.15951927395499388No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1010.15642181232479982No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1010.15642181232479982No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1010.15642181232479982No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1000.1548730815097028No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT990.15332435069460576No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC970.15022688906441173No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG970.15022688906441173No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT930.1440319658040236No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC900.1393857733587325No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT900.1393857733587325No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT890.1378370425436355No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC890.1378370425436355No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA880.13628831172853845No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA880.13628831172853845No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC870.13473958091344143No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG870.13473958091344143No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG860.13319085009834442No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT850.13164211928324737No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG850.13164211928324737No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG840.13009338846815036No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT840.13009338846815036No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC830.1285446576530533Illumina PCR Primer Index 10 (95% over 22bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT830.1285446576530533No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT810.12544719602285925No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT810.12544719602285925No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT810.12544719602285925No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT800.12389846520776224No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA800.12389846520776224No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT790.12234973439266522No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG790.12234973439266522No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC790.12234973439266522No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA780.12080100357756819No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA780.12080100357756819No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC770.11925227276247115No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA760.11770354194737412No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG760.11770354194737412No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC750.1161548111322771No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT740.11460608031718007No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG740.11460608031718007No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCC730.11305734950208304No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT730.11305734950208304No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA720.11150861868698603No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT720.11150861868698603No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG720.11150861868698603No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC720.11150861868698603No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA700.10841115705679195No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG700.10841115705679195No Hit
GGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAAC670.10376496461150088No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA670.10376496461150088No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG670.10376496461150088No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG670.10376496461150088No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC660.10221623379640385No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCT660.10221623379640385No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC660.10221623379640385No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG660.10221623379640385No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG660.10221623379640385No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG660.10221623379640385No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT660.10221623379640385No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT650.1006675029813068No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCT650.1006675029813068No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC650.1006675029813068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1050.037.01
AGGGTCT250.00545847229.59999836
GCCGTCT405.8550395E-527.75000236
TAATACG556.109276E-726.9090927
GTATCAA1550.025.0645162
CCGTCTT451.3044098E-424.66666837
GGTACTT451.3044098E-424.6666682
GGTAATA601.305043E-624.66666625
TATACAC400.001911616223.12500237
GCGGTAA652.618328E-622.76922823
AATACGG652.618328E-622.76922828
GTAATAC652.618328E-622.76922826
CGGTAAT652.618328E-622.76922824
GGGTACT502.6640174E-422.1999991
CGCGGTA704.979989E-621.14285922
CCGCGGT704.979989E-621.14285921
GCAGCCG806.7532164E-720.812517
TTATACA450.003787405320.55555736
GACTGTC450.003787405320.55555735
ATACCTG450.003787405320.55555735
Produced by FastQC (version 0.11.5)