FastQCFastQC Report
Fri 10 Feb 2017
ERR1632745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5590
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT200.35778175313059035TruSeq Adapter, Index 3 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA110.1967799642218247No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA90.16100178890876565No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA90.16100178890876565No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA80.14311270125223613No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA80.14311270125223613No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT70.12522361359570663No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA70.12522361359570663No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT60.1073345259391771No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA60.1073345259391771No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT60.1073345259391771No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT60.1073345259391771No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC60.1073345259391771No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA60.1073345259391771No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT60.1073345259391771No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA60.1073345259391771No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)