##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632745.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5590
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97942754919499	34.0	33.0	34.0	31.0	34.0
2	33.10375670840787	34.0	33.0	34.0	31.0	34.0
3	33.16851520572451	34.0	33.0	34.0	31.0	34.0
4	36.48157423971377	37.0	37.0	37.0	35.0	37.0
5	36.44293381037567	37.0	37.0	37.0	35.0	37.0
6	36.494812164579606	37.0	37.0	37.0	35.0	37.0
7	36.46118067978533	37.0	37.0	37.0	35.0	37.0
8	36.41717352415027	37.0	37.0	37.0	35.0	37.0
9	38.27781753130591	39.0	39.0	39.0	37.0	39.0
10	38.25957066189624	39.0	39.0	39.0	37.0	39.0
11	38.326833631484796	39.0	39.0	39.0	37.0	39.0
12	38.265295169946334	39.0	39.0	39.0	37.0	39.0
13	38.25992844364937	39.0	39.0	39.0	37.0	39.0
14	39.82486583184257	41.0	40.0	41.0	38.0	41.0
15	39.79105545617173	41.0	40.0	41.0	38.0	41.0
16	39.80966010733452	41.0	40.0	41.0	38.0	41.0
17	39.80518783542039	41.0	40.0	41.0	38.0	41.0
18	39.803220035778175	41.0	40.0	41.0	38.0	41.0
19	39.74007155635063	41.0	40.0	41.0	37.0	41.0
20	39.69731663685152	41.0	40.0	41.0	37.0	41.0
21	39.716100178890876	41.0	40.0	41.0	37.0	41.0
22	39.67030411449016	41.0	40.0	41.0	37.0	41.0
23	39.601788908765656	41.0	40.0	41.0	37.0	41.0
24	39.59910554561717	41.0	40.0	41.0	37.0	41.0
25	39.51932021466905	41.0	39.0	41.0	37.0	41.0
26	39.41610017889088	41.0	40.0	41.0	37.0	41.0
27	39.41574239713775	41.0	39.0	41.0	36.0	41.0
28	39.383542039355994	41.0	39.0	41.0	36.0	41.0
29	39.27584973166368	41.0	39.0	41.0	36.0	41.0
30	39.237745974955274	41.0	39.0	41.0	35.0	41.0
31	39.158497316636854	41.0	39.0	41.0	35.0	41.0
32	39.12737030411449	41.0	39.0	41.0	35.0	41.0
33	39.07638640429338	41.0	39.0	41.0	35.0	41.0
34	39.02683363148479	41.0	39.0	41.0	35.0	41.0
35	38.955277280858674	40.0	39.0	41.0	35.0	41.0
36	38.88837209302326	40.0	39.0	41.0	35.0	41.0
37	38.84400715563506	40.0	39.0	41.0	35.0	41.0
38	38.77728085867621	40.0	38.0	41.0	35.0	41.0
39	38.64436493738819	40.0	38.0	41.0	35.0	41.0
40	38.53953488372093	40.0	38.0	41.0	35.0	41.0
41	38.3964221824687	40.0	38.0	41.0	35.0	41.0
42	38.326833631484796	40.0	37.0	41.0	34.0	41.0
43	37.704293381037566	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	2.0
22	1.0
23	4.0
24	2.0
25	8.0
26	8.0
27	10.0
28	12.0
29	20.0
30	25.0
31	44.0
32	65.0
33	77.0
34	117.0
35	171.0
36	239.0
37	438.0
38	1050.0
39	3295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18604651162791	14.525939177101968	12.969588550983898	28.318425760286225
2	21.091234347048303	19.32021466905188	30.42933810375671	29.159212880143116
3	21.234347048300535	18.658318425760285	26.869409660107337	33.23792486583184
4	16.17173524150268	15.295169946332738	31.985688729874777	36.547406082289804
5	18.318425760286225	31.8783542039356	32.3613595706619	17.441860465116278
6	38.74776386404293	31.37745974955277	13.112701252236135	16.762075134168157
7	32.95169946332737	25.97495527728086	18.336314847942756	22.737030411449016
8	29.391771019677996	29.427549194991055	19.98211091234347	21.19856887298748
9	28.837209302325583	12.432915921288014	15.903398926654742	42.826475849731665
10	20.37567084078712	22.898032200357783	28.44364937388193	28.282647584973162
11	39.98211091234347	18.962432915921287	17.584973166368513	23.470483005366727
12	24.275491949910556	22.88014311270125	24.686940966010734	28.15742397137746
13	35.13416815742397	15.813953488372093	20.733452593917708	28.318425760286225
14	25.402504472271914	19.016100178890877	21.46690518783542	34.11449016100179
15	29.62432915921288	23.542039355992845	19.159212880143112	27.674418604651162
16	28.37209302325581	22.862254025044724	20.536672629695886	28.228980322003576
17	28.264758497316638	22.701252236135957	20.51878354203936	28.515205724508053
18	27.42397137745975	20.41144901610018	21.556350626118068	30.608228980322004
19	28.65831842576029	21.055456171735244	21.753130590339893	28.533094812164578
20	28.980322003577818	21.3953488372093	21.82468694096601	27.799642218246866
21	28.604651162790695	21.84257602862254	20.76923076923077	28.783542039355993
22	30.25044722719141	22.52236135957066	19.53488372093023	27.692307692307693
23	29.499105545617173	21.53846153846154	21.18067978533095	27.781753130590342
24	29.660107334525936	19.373881932021465	21.967799642218246	28.99821109123435
25	28.05008944543828	20.966010733452595	21.46690518783542	29.5169946332737
26	28.622540250447226	22.16457960644007	20.69767441860465	28.515205724508053
27	27.710196779964225	21.377459749552774	21.592128801431127	29.32021466905188
28	27.495527728085868	22.701252236135957	21.592128801431127	28.211091234347048
29	27.084078711985686	22.271914132379248	21.34168157423971	29.30232558139535
30	27.22719141323792	22.128801431127012	22.593917710196777	28.05008944543828
31	28.300536672629693	23.02325581395349	21.48479427549195	27.191413237924866
32	27.567084078711986	20.0	21.985688729874777	30.44722719141324
33	26.565295169946335	21.16279069767442	22.54025044722719	29.73166368515206
34	27.781753130590342	21.270125223613594	22.844364937388193	28.10375670840787
35	27.710196779964225	21.806797853309483	24.508050089445437	25.97495527728086
36	27.674418604651162	21.18067978533095	23.05903398926655	28.085867620751344
37	27.22719141323792	21.610017889087658	23.613595706618963	27.549194991055455
38	26.529516994633273	20.62611806797853	24.49016100178891	28.354203935599287
39	26.028622540250446	20.017889087656528	25.205724508050086	28.747763864042934
40	26.26118067978533	20.03577817531306	27.13774597495528	26.565295169946335
41	22.719141323792485	19.856887298747765	27.710196779964225	29.71377459749553
42	23.273703041144902	19.892665474060824	29.19499105545617	27.638640429338103
43	22.379248658318428	20.42933810375671	29.016100178890873	28.17531305903399
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	0.5
7	0.0
8	0.5
9	1.0
10	2.5
11	4.0
12	4.0
13	3.0
14	2.0
15	6.0
16	10.0
17	6.0
18	2.0
19	2.0
20	5.5
21	9.0
22	10.5
23	12.0
24	12.0
25	12.0
26	12.0
27	11.0
28	10.0
29	17.0
30	24.0
31	24.5
32	25.0
33	25.0
34	33.5
35	42.0
36	58.5
37	75.0
38	98.0
39	121.0
40	121.0
41	133.0
42	145.0
43	173.5
44	202.0
45	272.5
46	343.0
47	343.0
48	348.0
49	353.0
50	436.0
51	519.0
52	546.0
53	573.0
54	573.0
55	561.5
56	550.0
57	542.5
58	535.0
59	520.0
60	505.0
61	505.0
62	462.5
63	420.0
64	354.5
65	289.0
66	270.0
67	251.0
68	251.0
69	211.5
70	172.0
71	144.5
72	117.0
73	107.5
74	98.0
75	98.0
76	85.0
77	72.0
78	58.0
79	44.0
80	31.5
81	19.0
82	19.0
83	18.0
84	17.0
85	13.5
86	10.0
87	6.5
88	3.0
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5590.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.69767441860465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.70808678500985	84.99105545617174
2	4.497041420118343	8.15742397137746
3	1.0650887573964496	2.8980322003577816
4	0.21696252465483234	0.7871198568872988
5	0.19723865877712032	0.8944543828264758
6	0.15779092702169625	0.8586762075134168
7	0.03944773175542406	0.25044722719141327
8	0.03944773175542406	0.28622540250447226
9	0.03944773175542406	0.3220035778175313
>10	0.03944773175542406	0.554561717352415
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	20	0.35778175313059035	TruSeq Adapter, Index 3 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	11	0.1967799642218247	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	9	0.16100178890876565	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	9	0.16100178890876565	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	8	0.14311270125223613	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	8	0.14311270125223613	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	7	0.12522361359570663	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	7	0.12522361359570663	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	6	0.1073345259391771	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	6	0.1073345259391771	No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT	6	0.1073345259391771	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	6	0.1073345259391771	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	6	0.1073345259391771	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	6	0.1073345259391771	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	6	0.1073345259391771	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	6	0.1073345259391771	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.03577817531305903	0.0
27	0.0	0.0	0.0	0.05366726296958855	0.0
28	0.0	0.0	0.0	0.1967799642218247	0.0
29	0.0	0.0	0.0	0.3220035778175313	0.0
30	0.0	0.0	0.0	0.5008944543828264	0.0
31	0.0	0.0	0.0	0.8765652951699463	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE