FastQCFastQC Report
Fri 10 Feb 2017
ERR1632744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607929
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA39500.2456576129916184No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA33320.2072230801235627No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA31440.19553102158117677No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA28930.17992087959107647No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC25510.15865128373205534No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA24480.15224552825404605No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT23870.14845182840784638No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA23860.14838963660708898No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG23660.14714580059194154No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23350.14521785476846305No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT22620.1406778533131749No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC21160.13159785040259864No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA20540.12774195875564157No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT20230.12581401293216304No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA19670.12233127208975024No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA19670.12233127208975024No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC18100.11256715937084287No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA18030.11213181676554126No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG17570.10927099393070215No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC17300.10759181531025312No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA17130.1065345546973778No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16260.10112386803148646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.029.4296671
ATACGAC358.8709407E-426.4285723
ATACGGC2300.024.13043629
TATCTAG603.727476E-521.5833341
GTATCAA27900.020.6881732
CTTATAC13400.019.74253737
GCGGTAA16150.019.35913323
GTAACGT8550.019.04093626
TCAATGA7750.018.85806332
TAATACG16200.018.61419727
CGAACTA1002.8766954E-718.524
GTATAGA500.007036071718.51
CAATGAG7400.018.533
GGTCTAA609.2389993E-418.51
GGTAATA16950.018.44542925
AACTCCG17050.018.3372445
CGGTAAT17100.018.28362724
AGCCGCG17800.017.98033719
TGCGGGT9300.017.90322521
TTCTGCG9250.017.818
Produced by FastQC (version 0.11.5)