##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632744.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1607929
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.058363895420754	34.0	33.0	34.0	31.0	34.0
2	33.19634511225309	34.0	33.0	34.0	31.0	34.0
3	33.27259536957167	34.0	34.0	34.0	31.0	34.0
4	36.54927985004313	37.0	37.0	37.0	35.0	37.0
5	36.52120149583719	37.0	37.0	37.0	35.0	37.0
6	36.56659404737398	37.0	37.0	37.0	35.0	37.0
7	36.56334328194839	37.0	37.0	37.0	35.0	37.0
8	36.538520668512106	37.0	37.0	37.0	35.0	37.0
9	38.330627782694386	39.0	39.0	39.0	37.0	39.0
10	38.36617039682722	39.0	39.0	39.0	37.0	39.0
11	38.433647256812954	39.0	39.0	39.0	37.0	39.0
12	38.3966089298719	39.0	39.0	39.0	37.0	39.0
13	38.426148169477635	39.0	39.0	39.0	37.0	39.0
14	39.98511190481669	41.0	40.0	41.0	38.0	41.0
15	39.98249673959484	41.0	40.0	41.0	38.0	41.0
16	39.943881228586584	41.0	40.0	41.0	38.0	41.0
17	39.942430293874914	41.0	40.0	41.0	38.0	41.0
18	39.94332336813379	41.0	40.0	41.0	38.0	41.0
19	39.95020115937955	41.0	40.0	41.0	38.0	41.0
20	39.92343443025158	41.0	40.0	41.0	38.0	41.0
21	39.89249960663686	41.0	40.0	41.0	38.0	41.0
22	39.86174389540832	41.0	40.0	41.0	38.0	41.0
23	39.82712545143473	41.0	40.0	41.0	38.0	41.0
24	39.8064622256331	41.0	40.0	41.0	38.0	41.0
25	39.76529063161371	41.0	40.0	41.0	38.0	41.0
26	39.679571050711814	41.0	40.0	41.0	38.0	41.0
27	39.59719614485466	41.0	40.0	41.0	37.0	41.0
28	39.55672607434781	41.0	40.0	41.0	37.0	41.0
29	39.51403886614396	41.0	40.0	41.0	37.0	41.0
30	39.47277398442344	41.0	40.0	41.0	37.0	41.0
31	39.40921085445937	41.0	39.0	41.0	36.0	41.0
32	39.359741630382935	41.0	39.0	41.0	36.0	41.0
33	39.29353410505066	41.0	39.0	41.0	36.0	41.0
34	39.25503738038184	41.0	39.0	41.0	36.0	41.0
35	39.177911462508604	41.0	39.0	41.0	35.0	41.0
36	39.12207690762465	41.0	39.0	41.0	35.0	41.0
37	39.065142179785305	41.0	39.0	41.0	35.0	41.0
38	38.99145671232996	41.0	39.0	41.0	35.0	41.0
39	38.89378697691254	40.0	39.0	41.0	35.0	41.0
40	38.81429217334845	40.0	38.0	41.0	35.0	41.0
41	38.7596237147287	40.0	38.0	41.0	35.0	41.0
42	38.687527869700716	40.0	38.0	41.0	35.0	41.0
43	37.95724686848735	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	1.0
16	7.0
17	9.0
18	16.0
19	50.0
20	74.0
21	154.0
22	324.0
23	563.0
24	921.0
25	1324.0
26	1933.0
27	2627.0
28	3547.0
29	4955.0
30	6822.0
31	8782.0
32	11816.0
33	16162.0
34	23944.0
35	37701.0
36	60835.0
37	117494.0
38	311689.0
39	996177.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.49041531062628	16.144556134008408	12.317397099001262	28.04763145636406
2	21.44945454681146	19.100221465002498	31.879641451830278	27.570682536355772
3	21.63254720824116	19.335679622669907	28.67265905397564	30.35911411511329
4	15.904060440479647	15.546830736929303	33.66317791395018	34.885930908640866
5	17.467997654125273	32.49807671856158	33.008795786381114	17.02512984093203
6	34.89233666411888	33.09287910100508	15.299120794512694	16.71566344036335
7	30.657883525951707	27.20891283135014	19.697946862081597	22.43525678061656
8	27.602213779339756	30.288588613054436	20.20549414806251	21.9037034595433
9	28.24776467120128	12.698446262241678	17.430806957272367	41.62298210928468
10	19.559570105396446	23.05537122596831	29.740989807385777	27.644068861249472
11	39.679363952015294	19.048166927768577	18.757482451028622	22.514986669187508
12	22.97987037984886	23.209358124643565	26.666289369742074	27.1444821257655
13	35.24981513487225	17.375331870996792	20.68667211052229	26.688180883608663
14	24.46053277228037	19.569023259111564	22.872465139940882	33.09797882866719
15	29.802808457338603	23.675485671320065	19.86231979148333	26.659386079858006
16	26.036535195272926	23.442328610280676	22.358698673884234	28.162437520562168
17	27.019725373446214	23.352648033588547	21.6552472155176	27.972379377447638
18	26.74545953210621	21.271710380246887	23.294934042485707	28.6878960451612
19	28.289930712114774	22.065899675918526	22.96693448529133	26.67723512667537
20	29.897775337095105	20.66328799343752	22.512934339762513	26.926002329704858
21	27.703835181777304	21.954203201758286	21.728260389606753	28.613701226857653
22	28.526757089398846	22.548134898991187	21.042906745260517	27.882201266349448
23	28.52178174533826	21.962910053864316	21.945745116855285	27.56956308394214
24	28.379486905205393	21.35759725709282	22.122307639205463	28.140608198496324
25	28.62682369681746	21.723969155354496	22.542226677919235	27.10698046990881
26	28.476070771781593	22.359196208290292	22.041831449025423	27.122901570902698
27	27.47720825981744	21.60269514387762	23.674988136914006	27.24510845939093
28	26.763246387122813	22.670466171080935	21.94599388405831	28.620293557737934
29	27.62640638983438	23.149716187717242	21.89717332046378	27.3267041019846
30	27.39430658940786	22.004516368571	23.106866036995417	27.49431100502572
31	28.241732066527813	22.02398240220806	22.29675564032989	27.437529890934236
32	26.09375165196971	21.991829241216497	22.526429960526865	29.38798914628693
33	26.39706106426341	21.61867843667227	24.05087538069156	27.933385118372765
34	26.582827973125678	21.570355407483788	23.526909459310705	28.319907160079826
35	26.271060475928977	23.037273411947915	23.976058644380444	26.715607467742668
36	26.567528790139367	21.926465658620497	24.449027289140254	27.05697826209988
37	27.21270653119634	21.54572745438387	24.062878398237732	27.178687616182057
38	26.10743384813633	21.11579553574816	25.195515473630987	27.581255142484522
39	25.58514710537592	20.210407300322338	26.160732221385395	28.04371337291634
40	25.356032511385763	21.08992374663309	26.97351686548349	26.580526876497657
41	23.84545586278996	20.44779340381323	27.779957945904325	27.92679278749248
42	22.554851613472984	20.929220133476043	28.701329474124794	27.814598778926186
43	22.058125700823854	21.429801937772126	28.232900830820267	28.279171530583753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	8.5
2	12.0
3	27.0
4	42.0
5	42.0
6	60.5
7	79.0
8	80.5
9	82.0
10	130.5
11	179.0
12	179.0
13	356.5
14	534.0
15	949.5
16	1365.0
17	1357.5
18	1350.0
19	1350.0
20	1666.5
21	1983.0
22	1859.0
23	1735.0
24	2136.0
25	2537.0
26	2537.0
27	3216.0
28	3895.0
29	5317.0
30	6739.0
31	8336.0
32	9933.0
33	9933.0
34	13420.5
35	16908.0
36	19039.0
37	21170.0
38	27950.5
39	34731.0
40	34731.0
41	41708.5
42	48686.0
43	57913.5
44	67141.0
45	90257.5
46	113374.0
47	113374.0
48	127291.5
49	141209.0
50	157964.0
51	174719.0
52	180413.5
53	186108.0
54	186108.0
55	172856.5
56	159605.0
57	153167.5
58	146730.0
59	137183.0
60	127636.0
61	127636.0
62	117373.0
63	107110.0
64	90118.5
65	73127.0
66	64054.0
67	54981.0
68	54981.0
69	46929.0
70	38877.0
71	32822.5
72	26768.0
73	21444.0
74	16120.0
75	16120.0
76	13066.0
77	10012.0
78	7994.0
79	5976.0
80	4617.5
81	3259.0
82	3259.0
83	2487.5
84	1716.0
85	1266.5
86	817.0
87	607.5
88	398.0
89	398.0
90	294.0
91	190.0
92	121.5
93	53.0
94	40.0
95	27.0
96	27.0
97	18.5
98	10.0
99	5.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1607929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.17251699592734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.23711232636953	38.05202388250768
2	8.568256198228884	7.74099397479908
3	2.428554328564865	3.291117350478878
4	1.152765283465236	2.082932374385935
5	0.6740874940916028	1.52251143917975
6	0.4429004290351242	1.2004156294855595
7	0.3235945959423708	1.0232307669497887
8	0.24832656952874563	0.8974028946062076
9	0.19582240080779348	0.7961211655806011
>10	1.3539082080398332	12.231302406430968
>50	0.19415804563770692	6.1270316147241415
>100	0.15589231140926918	13.746228731697888
>500	0.015769023664151893	4.8356971070797155
>1k	0.008852785214962466	6.452990662093855
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3950	0.2456576129916184	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	3332	0.2072230801235627	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3144	0.19553102158117677	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2893	0.17992087959107647	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2551	0.15865128373205534	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2448	0.15224552825404605	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	2387	0.14845182840784638	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2386	0.14838963660708898	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2366	0.14714580059194154	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2335	0.14521785476846305	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	2262	0.1406778533131749	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	2116	0.13159785040259864	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	2054	0.12774195875564157	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	2023	0.12581401293216304	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1967	0.12233127208975024	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1967	0.12233127208975024	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	1810	0.11256715937084287	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	1803	0.11213181676554126	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1757	0.10927099393070215	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1730	0.10759181531025312	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1713	0.1065345546973778	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1626	0.10112386803148646	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	6.219180075737175E-5	0.0	0.0	0.0
4	0.0	6.219180075737175E-5	0.0	0.0	0.0
5	6.219180075737175E-5	6.219180075737175E-5	0.0	0.0	0.0
6	6.219180075737175E-5	6.219180075737175E-5	0.0	0.0	0.0
7	1.8657540227211524E-4	6.219180075737175E-5	6.219180075737175E-5	0.0	0.0
8	3.1095900378685877E-4	6.219180075737175E-5	6.219180075737175E-5	0.0	0.0
9	3.1095900378685877E-4	6.219180075737175E-5	6.219180075737175E-5	0.0	0.0
10	3.731508045442305E-4	6.219180075737175E-5	6.219180075737175E-5	0.0	0.0
11	3.731508045442305E-4	6.219180075737175E-5	6.219180075737175E-5	0.0	0.0
12	3.731508045442305E-4	1.243836015147435E-4	6.219180075737175E-5	0.0	0.0
13	3.731508045442305E-4	1.243836015147435E-4	6.219180075737175E-5	0.0	0.0
14	3.731508045442305E-4	1.243836015147435E-4	6.219180075737175E-5	0.0	0.0
15	3.731508045442305E-4	1.243836015147435E-4	6.219180075737175E-5	6.219180075737175E-5	0.0
16	3.731508045442305E-4	1.243836015147435E-4	6.219180075737175E-5	5.597262068163457E-4	0.0
17	3.731508045442305E-4	1.243836015147435E-4	6.219180075737175E-5	0.0010572606128753197	0.0
18	3.731508045442305E-4	1.243836015147435E-4	6.219180075737175E-5	0.0013060278159048068	0.0
19	3.731508045442305E-4	1.243836015147435E-4	6.219180075737175E-5	0.0018657540227211525	0.0
20	3.731508045442305E-4	1.243836015147435E-4	6.219180075737175E-5	0.002736439233324357	0.0
21	3.731508045442305E-4	1.243836015147435E-4	6.219180075737175E-5	0.004229042451501279	0.0
22	4.3534260530160226E-4	1.243836015147435E-4	6.219180075737175E-5	0.006156988274979803	0.0
23	4.3534260530160226E-4	1.8657540227211524E-4	6.219180075737175E-5	0.007711783293914097	0.0
24	4.3534260530160226E-4	1.8657540227211524E-4	6.219180075737175E-5	0.009764112718907364	0.0
25	4.3534260530160226E-4	1.8657540227211524E-4	6.219180075737175E-5	0.011132332335569543	0.0
26	4.3534260530160226E-4	1.8657540227211524E-4	6.219180075737175E-5	0.01492603218176922	0.0
27	4.3534260530160226E-4	1.8657540227211524E-4	6.219180075737175E-5	0.03352138060822337	0.0
28	4.3534260530160226E-4	1.8657540227211524E-4	6.219180075737175E-5	0.10796496611479736	0.0
29	4.3534260530160226E-4	1.8657540227211524E-4	6.219180075737175E-5	0.22308198931669246	0.0
30	4.3534260530160226E-4	1.8657540227211524E-4	6.219180075737175E-5	0.38291491726313787	0.0
31	4.3534260530160226E-4	1.8657540227211524E-4	6.219180075737175E-5	0.8364797201866501	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1955	0.0	29.429667	1
ATACGAC	35	8.8709407E-4	26.428572	3
ATACGGC	230	0.0	24.130436	29
TATCTAG	60	3.727476E-5	21.583334	1
GTATCAA	2790	0.0	20.688173	2
CTTATAC	1340	0.0	19.742537	37
GCGGTAA	1615	0.0	19.359133	23
GTAACGT	855	0.0	19.040936	26
TCAATGA	775	0.0	18.858063	32
TAATACG	1620	0.0	18.614197	27
CGAACTA	100	2.8766954E-7	18.5	24
GTATAGA	50	0.0070360717	18.5	1
CAATGAG	740	0.0	18.5	33
GGTCTAA	60	9.2389993E-4	18.5	1
GGTAATA	1695	0.0	18.445429	25
AACTCCG	1705	0.0	18.337244	5
CGGTAAT	1710	0.0	18.283627	24
AGCCGCG	1780	0.0	17.980337	19
TGCGGGT	930	0.0	17.903225	21
TTCTGCG	925	0.0	17.8	18
>>END_MODULE