FastQCFastQC Report
Fri 10 Feb 2017
ERR1632743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479830
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9250.19277660838213534No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7510.15651376529187422No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6910.14400933664006002No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6550.13650667944897152No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6400.13338057228601796No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6000.12504428651814184No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5850.1219181793551883No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5600.11670800075026572No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5230.1089969364149803No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5230.1089969364149803No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5150.10732967926140509No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5100.10628764354042057No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4910.10232790780067942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4800.029.6770821
ATACGGC601.3346944E-624.66666629
CTCTATG701.9180698E-723.7857131
CTAAGTA1600.023.12534
CATCTAA2100.022.9047631
TCTAAGT2150.022.37209333
CTTATAC5550.022.037
ATCTAAG2250.021.37777732
ACATCTA2250.021.37777730
TAAGTAC1750.021.14285735
GAAACAT2200.021.02272827
AGTACCC1500.020.96666737
AGACTAT450.003822608620.5555556
TCTACTC450.003822608620.5555553
GTCCTAA1101.7480488E-920.1818181
TAGAACA555.137093E-420.1818184
TAAACTA555.137093E-420.1818185
TAAGTCT555.137093E-420.18181835
GTGTAGA555.137093E-420.1818181
TTGCACT555.137093E-420.1818184
Produced by FastQC (version 0.11.5)