FastQCFastQC Report
Fri 10 Feb 2017
ERR1632742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT381.6295025728987993TruSeq Adapter, Index 5 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA150.6432246998284734No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC130.5574614065180103No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC110.4716981132075472Illumina Paired End PCR Primer 2 (95% over 23bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT90.38593481989708406No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA90.38593481989708406No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA90.38593481989708406No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC80.34305317324185247No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC80.34305317324185247No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT80.34305317324185247No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC80.34305317324185247No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA80.34305317324185247No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC80.34305317324185247No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC70.3001715265866209No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC70.3001715265866209No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC70.3001715265866209No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG60.2572898799313894No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA60.2572898799313894No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC60.2572898799313894No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA60.2572898799313894No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA60.2572898799313894No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC60.2572898799313894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.21440823327615782No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT50.21440823327615782No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC50.21440823327615782No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG50.21440823327615782No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG50.21440823327615782No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG50.21440823327615782No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG50.21440823327615782No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG50.21440823327615782No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT50.21440823327615782No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT50.21440823327615782No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC50.21440823327615782No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA50.21440823327615782No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA50.21440823327615782No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT40.17152658662092624No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA40.17152658662092624No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT40.17152658662092624No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA40.17152658662092624No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC40.17152658662092624No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG40.17152658662092624No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC40.17152658662092624No Hit
CCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTC40.17152658662092624No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA40.17152658662092624No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT40.17152658662092624No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT40.17152658662092624No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA40.17152658662092624No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT40.17152658662092624No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA40.17152658662092624No Hit
TTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCT40.17152658662092624No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA40.17152658662092624No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT40.17152658662092624No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC40.17152658662092624No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGA40.17152658662092624No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC40.17152658662092624No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGG40.17152658662092624No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT40.17152658662092624No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT40.17152658662092624No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC40.17152658662092624No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA30.1286449399656947No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG30.1286449399656947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30.1286449399656947No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA30.1286449399656947No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC30.1286449399656947No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA30.1286449399656947No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC30.1286449399656947No Hit
CCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCT30.1286449399656947No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG30.1286449399656947No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA30.1286449399656947No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA30.1286449399656947No Hit
CTCCCCAGGCGGTCGACTTAACGCGTTAGCTCCGGAAGCCACG30.1286449399656947No Hit
CTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCA30.1286449399656947No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG30.1286449399656947No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT30.1286449399656947No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT30.1286449399656947No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCT30.1286449399656947No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG30.1286449399656947No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC30.1286449399656947No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA30.1286449399656947No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG30.1286449399656947No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGC30.1286449399656947No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG30.1286449399656947No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG30.1286449399656947No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA30.1286449399656947No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC30.1286449399656947No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC30.1286449399656947No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC30.1286449399656947No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA30.1286449399656947No Hit
GTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATG30.1286449399656947No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGAT30.1286449399656947No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT30.1286449399656947No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC30.1286449399656947No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT30.1286449399656947No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT30.1286449399656947No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCTGTC30.1286449399656947No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG30.1286449399656947No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT30.1286449399656947No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACC30.1286449399656947No Hit
GTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAAT30.1286449399656947No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT30.1286449399656947No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC30.1286449399656947No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCCA30.1286449399656947No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA30.1286449399656947No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG30.1286449399656947No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA30.1286449399656947No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers