FastQCFastQC Report
Fri 10 Feb 2017
ERR1632741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28739
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT880.3062041128779707TruSeq Adapter, Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA560.1948571627405268No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA520.18093879397334633No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA460.16006124082257558No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC430.14962246424719022No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA380.1322245032882146No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC350.12178572671282925No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT340.11830613452103414No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA340.11830613452103414No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT320.11134695013744389No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA320.11134695013744389No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC310.10786735794564876No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA300.10438776575385365No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC290.10090817356205853RNA PCR Primer, Index 10 (95% over 22bp)
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG290.10090817356205853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA308.032972E-637.01
TGCCAGC352.308769E-531.71428712
GGCTAAC352.308769E-531.7142871
GCGGTAA303.5135308E-430.83333223
TAACTCC303.5135308E-430.8333324
CTAACTC303.5135308E-430.8333323
GCTAACT303.5135308E-430.8333322
TCCGTGC303.5135308E-430.8333328
CTCCGTG303.5135308E-430.8333327
CGGTAAT303.5135308E-430.83333224
TGTGAAC250.005408616729.59999837
CACGTGT250.005408616729.5999987
CCGTGCC358.659591E-426.4285729
AACTCCG358.659591E-426.4285725
CGTGCCA358.659591E-426.42857210
TAATACG358.659591E-426.42857227
AATACGG358.659591E-426.42857228
GTAATAC358.659591E-426.42857226
GTATCAA451.2798402E-424.6666682
CTTATAC400.001885934723.12537
Produced by FastQC (version 0.11.5)