##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632741.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28739
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.006054490413725	34.0	33.0	34.0	31.0	34.0
2	33.14596889244581	34.0	33.0	34.0	31.0	34.0
3	33.2122551236995	34.0	34.0	34.0	31.0	34.0
4	36.518285256967886	37.0	37.0	37.0	35.0	37.0
5	36.474268415741676	37.0	37.0	37.0	35.0	37.0
6	36.507428929329485	37.0	37.0	37.0	35.0	37.0
7	36.50461045965413	37.0	37.0	37.0	35.0	37.0
8	36.47833953860608	37.0	37.0	37.0	35.0	37.0
9	38.28490900866419	39.0	39.0	39.0	37.0	39.0
10	38.29799227530533	39.0	39.0	39.0	37.0	39.0
11	38.37840565085772	39.0	39.0	39.0	37.0	39.0
12	38.30477748007934	39.0	39.0	39.0	37.0	39.0
13	38.35780646508229	39.0	39.0	39.0	37.0	39.0
14	39.92591948223668	41.0	40.0	41.0	38.0	41.0
15	39.90678172518181	41.0	40.0	41.0	38.0	41.0
16	39.888131111033786	41.0	40.0	41.0	38.0	41.0
17	39.864539475973416	41.0	40.0	41.0	38.0	41.0
18	39.82591600264449	41.0	40.0	41.0	38.0	41.0
19	39.83805977939385	41.0	40.0	41.0	38.0	41.0
20	39.82351508403215	41.0	40.0	41.0	38.0	41.0
21	39.77239987473468	41.0	40.0	41.0	38.0	41.0
22	39.74828630084554	41.0	40.0	41.0	38.0	41.0
23	39.70590486794948	41.0	40.0	41.0	38.0	41.0
24	39.68882007028776	41.0	40.0	41.0	37.0	41.0
25	39.62489996172449	41.0	40.0	41.0	37.0	41.0
26	39.53766658547618	41.0	40.0	41.0	37.0	41.0
27	39.461985455304635	41.0	39.0	41.0	37.0	41.0
28	39.406659939455096	41.0	39.0	41.0	37.0	41.0
29	39.37165524200564	41.0	39.0	41.0	36.0	41.0
30	39.298166254914925	41.0	39.0	41.0	36.0	41.0
31	39.245206861755804	41.0	39.0	41.0	36.0	41.0
32	39.19697971397752	41.0	39.0	41.0	35.0	41.0
33	39.1304499112704	41.0	39.0	41.0	35.0	41.0
34	39.07000939489892	41.0	39.0	41.0	35.0	41.0
35	39.01381398100143	41.0	39.0	41.0	35.0	41.0
36	38.95469570966283	40.0	39.0	41.0	35.0	41.0
37	38.875465395455656	40.0	39.0	41.0	35.0	41.0
38	38.840286718396605	40.0	39.0	41.0	35.0	41.0
39	38.72556456383312	40.0	38.0	41.0	35.0	41.0
40	38.63137200320122	40.0	38.0	41.0	35.0	41.0
41	38.55722189359407	40.0	38.0	41.0	35.0	41.0
42	38.4681095375622	40.0	38.0	41.0	35.0	41.0
43	37.71481262396047	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	3.0
22	2.0
23	14.0
24	19.0
25	25.0
26	39.0
27	50.0
28	85.0
29	126.0
30	122.0
31	194.0
32	238.0
33	359.0
34	502.0
35	761.0
36	1247.0
37	2212.0
38	5832.0
39	16905.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82198406346776	14.875256619924146	11.353909321827482	29.948849994780613
2	21.375134834197432	17.850307943908973	30.898778663140682	29.875778558752913
3	22.21719614461185	18.483593722815687	27.20345175545426	32.0957583771182
4	16.750756811301713	14.1236647064964	30.703921500400156	38.42165698180173
5	17.484950763770485	32.07140123177564	32.60377883712029	17.83986916733359
6	38.230279411253	31.55642158738996	13.70263405128919	16.510664950067852
7	32.374125752461815	26.37530881380702	18.532308013500817	22.718257420230348
8	29.392115244093393	29.969727547931384	18.967257037475207	21.67090017050002
9	28.42130902258255	12.237725738543443	16.294930234176555	43.046035004697444
10	19.708410174327568	22.321583910365707	28.93280907477644	29.03719684053029
11	40.91304499112704	17.975573262813597	17.43971606527715	23.671665680782212
12	24.746859668046906	22.523400257489822	24.78513518215665	27.944604892306625
13	35.49184035631024	16.21142002157347	19.53095097254602	28.765788649570272
14	25.254880128048995	18.42096106336337	21.0410939837851	35.28306482480254
15	30.756115383277077	22.2519920665298	18.803716204460837	28.18817634573228
16	28.4595845366923	22.29026758063955	19.882389783917326	29.36775809875083
17	28.104666133129196	22.450328821462122	20.51915515501583	28.925849890392847
18	27.40526810257838	20.31385921569992	22.2554716587216	30.025401023000104
19	29.76791120080727	20.4704408643307	21.079369497894845	28.68227843696719
20	29.976686732314974	19.72928772747834	21.66046139392463	28.633564146282055
21	29.252931556421586	20.814920491318418	20.36953269076864	29.562615261491352
22	31.024043982045303	21.256828699676397	19.38132850829883	28.33779880997947
23	29.552176484915964	21.201155224607675	19.97981836528759	29.26684992518877
24	29.757472424231878	20.230349003096837	20.174675528028114	29.837503044643164
25	29.82010508368419	20.672257211454816	21.524757298444623	27.982880406416367
26	29.945370402588818	20.787083753784056	21.072410313511256	28.195135530115873
27	28.713594766693344	20.734889870907132	22.307665541598524	28.243849820801003
28	27.93068652353944	21.305542990361527	21.180277671456903	29.583492814642128
29	29.026758063954905	21.78224712063746	20.752287831866106	28.43870698354153
30	28.417829430390757	20.352134729809666	22.06061449598107	29.169421343818502
31	28.80058457148822	21.121124604196385	21.406451163923588	28.6718396603918
32	26.952921117645012	20.79752253035944	21.740492014335917	30.509064337659623
33	27.582727304359928	20.62006332857789	22.770451303107276	29.026758063954905
34	27.58968648874352	20.8984307039215	22.906155398587284	28.605727408747693
35	27.47833953860608	21.281185845018964	23.685584049549394	27.55489056682557
36	27.631441595045057	20.15727756706914	23.685584049549394	28.525696788336408
37	26.430982288875743	20.20599185775427	24.249277984620203	29.113747868749783
38	26.751104770520897	19.576185671039354	24.701624969553567	28.971084588886182
39	25.846410800654162	18.504471275966456	26.239604718327016	29.409513205052367
40	25.373186262570023	19.002052959393158	28.090747764362018	27.534013013674794
41	23.821288145029403	18.584501896377745	28.369115139705624	29.225094818887225
42	22.286787988447752	19.155155015832147	29.92797244162984	28.630084554090264
43	21.580430773513346	19.24910400501061	29.562615261491352	29.60784995998469
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	2.5
7	3.0
8	1.5
9	0.0
10	1.0
11	2.0
12	2.0
13	4.0
14	6.0
15	10.5
16	15.0
17	11.0
18	7.0
19	7.0
20	15.5
21	24.0
22	21.0
23	18.0
24	17.5
25	17.0
26	17.0
27	26.5
28	36.0
29	45.5
30	55.0
31	80.5
32	106.0
33	106.0
34	146.0
35	186.0
36	245.0
37	304.0
38	388.5
39	473.0
40	473.0
41	573.0
42	673.0
43	811.5
44	950.0
45	1240.0
46	1530.0
47	1530.0
48	1773.0
49	2016.0
50	2288.5
51	2561.0
52	2870.5
53	3180.0
54	3180.0
55	3098.0
56	3016.0
57	2859.5
58	2703.0
59	2646.0
60	2589.0
61	2589.0
62	2495.0
63	2401.0
64	2026.0
65	1651.0
66	1454.5
67	1258.0
68	1258.0
69	1053.0
70	848.0
71	780.5
72	713.0
73	636.0
74	559.0
75	559.0
76	509.0
77	459.0
78	327.0
79	195.0
80	143.5
81	92.0
82	92.0
83	64.5
84	37.0
85	31.0
86	25.0
87	17.5
88	10.0
89	10.0
90	12.0
91	14.0
92	8.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	28739.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.95253836250392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.07366684991753	67.65371098507255
2	5.644126809602346	8.57371516058318
3	1.9882719442917351	4.530429033717248
4	1.0628550485614805	3.229061553985873
5	0.5451713395638629	2.0703573541180975
6	0.3802455561663918	1.7328369115139706
7	0.2748763056624519	1.4614287205539511
8	0.23364485981308408	1.4196736142524096
9	0.17408832691955287	1.1900205295939315
>10	0.609309144218435	7.456766067016946
>50	0.013743815283122596	0.6820000695918438
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	88	0.3062041128779707	TruSeq Adapter, Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	56	0.1948571627405268	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	52	0.18093879397334633	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	46	0.16006124082257558	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	43	0.14962246424719022	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	38	0.1322245032882146	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	35	0.12178572671282925	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	34	0.11830613452103414	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	34	0.11830613452103414	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	32	0.11134695013744389	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	32	0.11134695013744389	No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	31	0.10786735794564876	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	30	0.10438776575385365	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	29	0.10090817356205853	RNA PCR Primer, Index 10 (95% over 22bp)
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	29	0.10090817356205853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0034795921917951216	0.0
20	0.0	0.0	0.0	0.0034795921917951216	0.0
21	0.0	0.0	0.0	0.0034795921917951216	0.0
22	0.0	0.0	0.0	0.0034795921917951216	0.0
23	0.0	0.0	0.0	0.0034795921917951216	0.0
24	0.0	0.0	0.0	0.010438776575385365	0.0
25	0.0	0.0	0.0	0.010438776575385365	0.0
26	0.0	0.0	0.0	0.010438776575385365	0.0
27	0.0	0.0	0.0	0.02435714534256585	0.0
28	0.0	0.0	0.0	0.14266327986359997	0.0
29	0.0	0.0	0.0	0.33404085041233167	0.0
30	0.0	0.0	0.0	0.5950102647969658	0.0
31	0.0	0.0	0.0	1.2143776749364974	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30	8.032972E-6	37.0	1
TGCCAGC	35	2.308769E-5	31.714287	12
GGCTAAC	35	2.308769E-5	31.714287	1
GCGGTAA	30	3.5135308E-4	30.833332	23
TAACTCC	30	3.5135308E-4	30.833332	4
CTAACTC	30	3.5135308E-4	30.833332	3
GCTAACT	30	3.5135308E-4	30.833332	2
TCCGTGC	30	3.5135308E-4	30.833332	8
CTCCGTG	30	3.5135308E-4	30.833332	7
CGGTAAT	30	3.5135308E-4	30.833332	24
TGTGAAC	25	0.0054086167	29.599998	37
CACGTGT	25	0.0054086167	29.599998	7
CCGTGCC	35	8.659591E-4	26.428572	9
AACTCCG	35	8.659591E-4	26.428572	5
CGTGCCA	35	8.659591E-4	26.428572	10
TAATACG	35	8.659591E-4	26.428572	27
AATACGG	35	8.659591E-4	26.428572	28
GTAATAC	35	8.659591E-4	26.428572	26
GTATCAA	45	1.2798402E-4	24.666668	2
CTTATAC	40	0.0018859347	23.125	37
>>END_MODULE