ERR1632740.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632740.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	202701
Sequences flagged as poor quality	0
Sequence length	43
%GC	56

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	671	0.3310294473140241	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	490	0.2417353639103902	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	445	0.21953517742882372	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	391	0.192894953650944	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	361	0.17809482932989973	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	359	0.17710815437516342	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	354	0.1746414669883227	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	328	0.16181469257675096	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	308	0.1519479430293881	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	308	0.1519479430293881	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	296	0.1460278933009704	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	290	0.14306786843676153	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	288	0.14208119348202525	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	287	0.14158785600465712	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	282	0.13912116861781637	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	277	0.13665448123097568	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	274	0.13517446879887124	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	268	0.1322144439346624	TruSeq Adapter, Index 7 (95% over 21bp)
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	263	0.12974775654782167	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	254	0.12530771925150838	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	245	0.1208676819551951	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	244	0.12037434447782695	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	228	0.11248094483993665	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	220	0.10853424502099152	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	214	0.10557422015678265	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	204	0.10064084538310121	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTACG	20	0.0018383624	37.0	8
GGTATCA	290	0.0	29.982758	1
GGTGTTC	25	0.0054858015	29.6	8
TAAGGAG	25	0.0054858015	29.6	4
ATACGGC	50	2.702127E-7	29.6	29
AAGTACC	35	8.844112E-4	26.42857	36
CATCTAA	45	1.3179664E-4	24.666668	31
ACATCTA	50	2.691477E-4	22.2	30
TTATACA	130	1.8189894E-12	21.346153	37
GCCGTCT	70	5.0677972E-6	21.142857	36
AACGTCA	125	1.8189894E-11	20.72	28
GTATCAA	420	0.0	20.702381	2
ATACACA	45	0.0038151082	20.555557	37
GGACGTA	55	5.1230355E-4	20.181818	27
TACGGCT	75	9.204186E-6	19.733332	30
ACGTCAA	135	5.4569682E-11	19.185184	29
GTAACGT	145	7.2759576E-12	19.13793	26
ATCTCCG	70	1.21292265E-4	18.5	2
ATCTAAG	60	9.2010864E-4	18.5	32
TGGACGT	60	9.2010864E-4	18.5	26

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content