##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632740.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	202701
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02283659182737	34.0	33.0	34.0	31.0	34.0
2	33.16382257610964	34.0	33.0	34.0	31.0	34.0
3	33.24422178479632	34.0	34.0	34.0	31.0	34.0
4	36.528157236520784	37.0	37.0	37.0	35.0	37.0
5	36.49428961869946	37.0	37.0	37.0	35.0	37.0
6	36.53248873957208	37.0	37.0	37.0	35.0	37.0
7	36.535927301789336	37.0	37.0	37.0	35.0	37.0
8	36.510732556820145	37.0	37.0	37.0	35.0	37.0
9	38.31732946556751	39.0	39.0	39.0	37.0	39.0
10	38.32992437136472	39.0	39.0	39.0	37.0	39.0
11	38.40198124330911	39.0	39.0	39.0	37.0	39.0
12	38.35655472839305	39.0	39.0	39.0	37.0	39.0
13	38.40254364803331	39.0	39.0	39.0	37.0	39.0
14	39.9367936023996	41.0	40.0	41.0	38.0	41.0
15	39.93526425621975	41.0	40.0	41.0	38.0	41.0
16	39.88885599972373	41.0	40.0	41.0	38.0	41.0
17	39.88809626000858	41.0	40.0	41.0	38.0	41.0
18	39.893266436771405	41.0	40.0	41.0	38.0	41.0
19	39.8913177537358	41.0	40.0	41.0	38.0	41.0
20	39.86375498887524	41.0	40.0	41.0	38.0	41.0
21	39.82437679143172	41.0	40.0	41.0	38.0	41.0
22	39.795067611901274	41.0	40.0	41.0	38.0	41.0
23	39.75208805087296	41.0	40.0	41.0	38.0	41.0
24	39.74107182500333	41.0	40.0	41.0	38.0	41.0
25	39.684476149599654	41.0	40.0	41.0	37.0	41.0
26	39.60057917819843	41.0	40.0	41.0	37.0	41.0
27	39.501245677130356	41.0	39.0	41.0	37.0	41.0
28	39.45396421329939	41.0	39.0	41.0	37.0	41.0
29	39.403071519134095	41.0	39.0	41.0	36.0	41.0
30	39.36208010813957	41.0	39.0	41.0	36.0	41.0
31	39.292514590455895	41.0	39.0	41.0	36.0	41.0
32	39.23217941697377	41.0	39.0	41.0	36.0	41.0
33	39.15017686148563	41.0	39.0	41.0	35.0	41.0
34	39.10776957193107	41.0	39.0	41.0	35.0	41.0
35	39.032693474625184	41.0	39.0	41.0	35.0	41.0
36	38.970754954341615	40.0	39.0	41.0	35.0	41.0
37	38.90711441976113	40.0	39.0	41.0	35.0	41.0
38	38.82611333935205	40.0	38.0	41.0	35.0	41.0
39	38.72170832901663	40.0	38.0	41.0	35.0	41.0
40	38.64102791796785	40.0	38.0	41.0	35.0	41.0
41	38.59136856749597	40.0	38.0	41.0	35.0	41.0
42	38.5230561270048	40.0	38.0	41.0	35.0	41.0
43	37.77113581087414	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	5.0
19	4.0
20	13.0
21	16.0
22	51.0
23	81.0
24	132.0
25	182.0
26	249.0
27	386.0
28	519.0
29	681.0
30	876.0
31	1259.0
32	1648.0
33	2195.0
34	3284.0
35	5297.0
36	8409.0
37	16092.0
38	41206.0
39	120115.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.725980631570636	15.342795546149254	11.940740302218538	28.990483520061566
2	22.25988031632799	18.563302598408495	30.12713306791777	29.049684017345744
3	21.987558028820775	18.29147364837865	27.80006018717224	31.92090813562834
4	16.095135199135672	15.148420580066205	32.93224996423304	35.82419425656509
5	18.206126264793955	31.96382849615937	32.17497693647293	17.655068302573742
6	35.617979191025206	32.51340644594748	14.94763222677737	16.920982136249947
7	30.973206841604135	26.17845989906315	18.938239081208284	23.91009417812443
8	28.145396421329938	29.54795486948757	19.7803661550757	22.52628255410679
9	28.02304872694264	11.978233950498518	16.723647145302685	43.275070177256154
10	20.111888939867097	21.964864504861843	28.34766478705088	29.57558176822019
11	41.347600653178816	18.20563292731659	17.643721540594274	22.803044878910317
12	23.36791629049684	22.500135667806276	25.866670613366487	28.2652774283304
13	36.63228104449411	16.527792166787535	19.251015041859684	27.588911746858674
14	24.665393855975058	18.607209633894257	21.765062826527743	34.96233368360294
15	30.98208691619676	22.451788595024198	18.51347551319431	28.052648975584727
16	26.718171099303902	22.69895067118564	21.27912541132012	29.303752818190336
17	28.096062673593124	22.434028445838944	20.48978544753109	28.980123433036837
18	27.544511373895542	20.066501891949226	22.052185238356003	30.336801495799232
19	29.130591363634124	21.33783257112693	21.923424156762916	27.60815190847603
20	31.024020601773056	19.65851179816577	21.233738363402253	28.08372923665892
21	28.688560983912264	20.531225795630018	20.72116072441675	30.059052496040966
22	29.790183570875328	21.395553055979004	19.70192549617417	29.1123378769715
23	30.020078835328885	20.68958712586519	20.57315948120631	28.717174557599616
24	29.185845161099355	20.19575631101968	20.931815827252947	29.686582700628016
25	29.55436825669336	20.860281893034568	21.4389667539874	28.146383096284676
26	29.268232519819833	21.452780203353708	20.904188928520334	28.374798348306125
27	28.06794243738314	20.781347896655667	23.024553406248614	28.12615625971258
28	27.593845121632356	21.77640958850721	20.82229490727722	29.807450382583212
29	28.462612419277654	22.623470037148312	20.868668630149827	28.045248913424203
30	28.14095638403363	20.657520189836262	22.59781648832517	28.603706937804947
31	29.100004440037296	21.22140492646805	21.253965199974346	28.424625433520305
32	27.06301399598423	20.87014864258193	21.810943211922982	30.255894149510855
33	27.228775388379926	20.695507175593608	23.212021647648506	28.863695788377957
34	27.311162747100408	20.710800637392023	22.93969936014129	29.03833725536628
35	26.6283836784229	22.13457259707648	23.60422494215618	27.632818782344437
36	27.124187843177882	20.508038934193713	24.283057311014748	28.084715911613657
37	27.340269658265132	19.861766838841447	24.235203575710035	28.56275992718339
38	26.34175460407201	19.370896048860146	25.67673568457975	28.6106136624881
39	25.653548823143446	18.666903468655804	26.575103230867143	29.104444477333608
40	25.14245119659005	19.56033764016951	28.041795551082625	27.255415612157808
41	23.395543189229457	19.141494121883955	28.621467086990197	28.841495601896387
42	21.697968929605675	19.875086950730385	30.103946206481467	28.32299791318247
43	20.860281893034568	20.43255830015639	29.32743301710401	29.379726789705035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.0
4	4.0
5	4.0
6	7.5
7	11.0
8	10.0
9	9.0
10	10.5
11	12.0
12	12.0
13	27.0
14	42.0
15	71.5
16	101.0
17	92.0
18	83.0
19	83.0
20	118.5
21	154.0
22	121.0
23	88.0
24	93.0
25	98.0
26	98.0
27	136.5
28	175.0
29	245.0
30	315.0
31	433.0
32	551.0
33	551.0
34	830.5
35	1110.0
36	1384.0
37	1658.0
38	2280.5
39	2903.0
40	2903.0
41	3628.5
42	4354.0
43	5492.0
44	6630.0
45	9540.0
46	12450.0
47	12450.0
48	14316.5
49	16183.0
50	18553.0
51	20923.0
52	21940.5
53	22958.0
54	22958.0
55	22041.5
56	21125.0
57	20751.0
58	20377.0
59	19422.0
60	18467.0
61	18467.0
62	17161.5
63	15856.0
64	13353.5
65	10851.0
66	9698.5
67	8546.0
68	8546.0
69	7267.0
70	5988.0
71	5095.0
72	4202.0
73	3390.5
74	2579.0
75	2579.0
76	2107.0
77	1635.0
78	1303.0
79	971.0
80	790.5
81	610.0
82	610.0
83	486.0
84	362.0
85	263.5
86	165.0
87	129.0
88	93.0
89	93.0
90	63.5
91	34.0
92	29.0
93	24.0
94	13.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	202701.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.301888133970344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.0735154355982	47.60027921917022
2	6.863108839225383	7.590857545562105
3	2.1765155196928667	3.6109625337571574
4	1.0585751892972135	2.3416482679964394
5	0.7181584075059277	1.9857757957181557
6	0.4776603450699814	1.5849311381456275
7	0.33903307454092657	1.3124418413384846
8	0.28219254677403116	1.2484624523150132
9	0.2532748238163632	1.2605918376459206
>10	1.5146625542728085	16.36838621236403
>50	0.1575488350188197	6.074660285894105
>100	0.08475728466202324	8.650986797849193
>500	9.971445254355676E-4	0.3700160722435497
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	671	0.3310294473140241	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	490	0.2417353639103902	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	445	0.21953517742882372	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	391	0.192894953650944	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	361	0.17809482932989973	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	359	0.17710815437516342	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	354	0.1746414669883227	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	328	0.16181469257675096	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	308	0.1519479430293881	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	308	0.1519479430293881	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	296	0.1460278933009704	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	290	0.14306786843676153	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	288	0.14208119348202525	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	287	0.14158785600465712	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	282	0.13912116861781637	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	277	0.13665448123097568	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	274	0.13517446879887124	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	268	0.1322144439346624	TruSeq Adapter, Index 7 (95% over 21bp)
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	263	0.12974775654782167	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	254	0.12530771925150838	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	245	0.1208676819551951	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	244	0.12037434447782695	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	228	0.11248094483993665	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	220	0.10853424502099152	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	214	0.10557422015678265	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	204	0.10064084538310121	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.933374773681432E-4	0.0	0.0	0.0	0.0
9	4.933374773681432E-4	0.0	0.0	0.0	0.0
10	4.933374773681432E-4	0.0	0.0	0.0	0.0
11	4.933374773681432E-4	0.0	0.0	4.933374773681432E-4	0.0
12	4.933374773681432E-4	0.0	0.0	4.933374773681432E-4	0.0
13	4.933374773681432E-4	0.0	0.0	4.933374773681432E-4	0.0
14	4.933374773681432E-4	0.0	0.0	4.933374773681432E-4	0.0
15	4.933374773681432E-4	0.0	0.0	4.933374773681432E-4	0.0
16	4.933374773681432E-4	0.0	0.0	4.933374773681432E-4	0.0
17	4.933374773681432E-4	0.0	0.0	9.866749547362864E-4	0.0
18	4.933374773681432E-4	0.0	0.0	0.0014800124321044297	0.0
19	4.933374773681432E-4	0.0	0.0	0.001973349909472573	0.0
20	4.933374773681432E-4	0.0	0.0	0.001973349909472573	0.0
21	4.933374773681432E-4	0.0	0.0	0.001973349909472573	0.0
22	4.933374773681432E-4	0.0	0.0	0.0034533623415770025	0.0
23	4.933374773681432E-4	0.0	0.0	0.004933374773681432	0.0
24	4.933374773681432E-4	0.0	0.0	0.006413387205785862	0.0
25	4.933374773681432E-4	0.0	0.0	0.008386737115258435	0.0
26	4.933374773681432E-4	0.0	0.0	0.01085342450209915	0.0
27	4.933374773681432E-4	0.0	0.0	0.043907035485764745	0.0
28	4.933374773681432E-4	0.0	0.0	0.16872141725990497	0.0
29	4.933374773681432E-4	0.0	0.0	0.3537229712729587	0.0
30	4.933374773681432E-4	0.0	0.0	0.6171651841875472	0.0
31	4.933374773681432E-4	0.0	0.0	1.3043842901613707	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTACG	20	0.0018383624	37.0	8
GGTATCA	290	0.0	29.982758	1
GGTGTTC	25	0.0054858015	29.6	8
TAAGGAG	25	0.0054858015	29.6	4
ATACGGC	50	2.702127E-7	29.6	29
AAGTACC	35	8.844112E-4	26.42857	36
CATCTAA	45	1.3179664E-4	24.666668	31
ACATCTA	50	2.691477E-4	22.2	30
TTATACA	130	1.8189894E-12	21.346153	37
GCCGTCT	70	5.0677972E-6	21.142857	36
AACGTCA	125	1.8189894E-11	20.72	28
GTATCAA	420	0.0	20.702381	2
ATACACA	45	0.0038151082	20.555557	37
GGACGTA	55	5.1230355E-4	20.181818	27
TACGGCT	75	9.204186E-6	19.733332	30
ACGTCAA	135	5.4569682E-11	19.185184	29
GTAACGT	145	7.2759576E-12	19.13793	26
ATCTCCG	70	1.21292265E-4	18.5	2
ATCTAAG	60	9.2010864E-4	18.5	32
TGGACGT	60	9.2010864E-4	18.5	26
>>END_MODULE