FastQCFastQC Report
Fri 10 Feb 2017
ERR1632739.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632739.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058941
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA26470.24996671202644907No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21170.199916709240647No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG17560.1658260469657894No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17420.16450397142050407No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC17280.16318189587521875No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC16340.1543051029283029No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG16110.15213312167533413No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG14340.13541830942422667No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA13000.12276415777649557No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC12860.12144208223121024No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12780.12068661049104717No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT12750.12040330858848604No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT12630.11927010097824146No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA12490.11794802543295614No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT12340.1165315159201504No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12300.1161537800500689No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG11730.11077104390140717No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11460.10822132677835686No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA11410.10774915694075496No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11390.10756028900571421No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC11160.10538830775274544No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA11120.1050105718826639No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11080.10463283601258239No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10960.1034996284023378No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC10840.10236642079209322No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC10770.10170538301945056No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA10740.10142208111688941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGAGT303.59929E-430.8333324
GGTATCA10250.030.3219531
ATACGGC1700.028.29411729
CGTTACG400.001931087223.12500216
TAGAACC753.7401878E-722.2000034
TTTAAAC502.7019082E-422.1999993
TCTATAC603.7259313E-521.5833323
GCGGTAA11750.021.41276623
TACGGCT2250.021.37777730
GACCGTT1059.822543E-1021.1428597
GTATCAA14700.021.0170082
CGTGATA450.003825611420.55555737
TAGTCTC450.003825611420.5555575
TAATACG11150.020.24215127
CGTGCCA12450.020.20883410
AACTCCG12500.020.1285
CGGTAAT12550.019.900424
AGCCGCG12700.019.81102419
GGTAATA12800.019.80078125
TCCGTGC13050.019.2796948
Produced by FastQC (version 0.11.5)