FastQCFastQC Report
Fri 10 Feb 2017
ERR1632738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18924
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA740.39103783555273725No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA420.22194039315155356No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT420.22194039315155356TruSeq Adapter, Index 1 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA400.2113718030014796No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT250.13210737687592475No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC240.12682308180088775No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA240.12682308180088775No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC240.12682308180088775No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA230.12153878672585076No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA230.12153878672585076No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC220.11625449165081378No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC220.11625449165081378No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA210.11097019657577678No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC210.11097019657577678No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT200.1056859015007398No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT190.1004016064257028No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA190.1004016064257028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTA1058.411156E-615.85714237
GTCTCTT1602.3205394E-612.7187537
CCTGTCT1358.998784E-512.33333434
TGTCTCT1808.6612035E-611.30555536
CTGTCTC2053.6565863E-59.9268335
Produced by FastQC (version 0.11.5)